ApoE receptor 2 regulates synapse and dendritic spine formation.
<h4>Background</h4>Apolipoprotein E receptor 2 (ApoEr2) is a postsynaptic protein involved in long-term potentiation (LTP), learning, and memory through unknown mechanisms. We examined the biological effects of ApoEr2 on synapse and dendritic spine formation-processes critical for learni...
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| Format: | Article |
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Public Library of Science (PLoS)
2011-02-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017203&type=printable |
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| author | Sonya B Dumanis Hyun-Jung Cha Jung Min Song Justin H Trotter Matthew Spitzer Ji-Yun Lee Edwin J Weeber R Scott Turner Daniel T S Pak G William Rebeck Hyang-Sook Hoe |
| author_facet | Sonya B Dumanis Hyun-Jung Cha Jung Min Song Justin H Trotter Matthew Spitzer Ji-Yun Lee Edwin J Weeber R Scott Turner Daniel T S Pak G William Rebeck Hyang-Sook Hoe |
| author_sort | Sonya B Dumanis |
| collection | DOAJ |
| description | <h4>Background</h4>Apolipoprotein E receptor 2 (ApoEr2) is a postsynaptic protein involved in long-term potentiation (LTP), learning, and memory through unknown mechanisms. We examined the biological effects of ApoEr2 on synapse and dendritic spine formation-processes critical for learning and memory.<h4>Methodology/principal findings</h4>In a heterologous co-culture synapse assay, overexpression of ApoEr2 in COS7 cells significantly increased colocalization with synaptophysin in primary hippocampal neurons, suggesting that ApoEr2 promotes interaction with presynaptic structures. In primary neuronal cultures, overexpression of ApoEr2 increased dendritic spine density. Consistent with our in vitro findings, ApoEr2 knockout mice had decreased dendritic spine density in cortical layers II/III at 1 month of age. We also tested whether the interaction between ApoEr2 and its cytoplasmic adaptor proteins, specifically X11α and PSD-95, affected synapse and dendritic spine formation. X11α decreased cell surface levels of ApoEr2 along with synapse and dendritic spine density. In contrast, PSD-95 increased cell surface levels of ApoEr2 as well as synapse and dendritic spine density.<h4>Conclusions/significance</h4>These results suggest that ApoEr2 plays important roles in structure and function of CNS synapses and dendritic spines, and that these roles are modulated by cytoplasmic adaptor proteins X11α and PSD-95. |
| format | Article |
| id | doaj-art-746b792ebb2b4594be3396e2f48cfa3a |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-746b792ebb2b4594be3396e2f48cfa3a2025-08-20T03:45:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-02-0162e1720310.1371/journal.pone.0017203ApoE receptor 2 regulates synapse and dendritic spine formation.Sonya B DumanisHyun-Jung ChaJung Min SongJustin H TrotterMatthew SpitzerJi-Yun LeeEdwin J WeeberR Scott TurnerDaniel T S PakG William RebeckHyang-Sook Hoe<h4>Background</h4>Apolipoprotein E receptor 2 (ApoEr2) is a postsynaptic protein involved in long-term potentiation (LTP), learning, and memory through unknown mechanisms. We examined the biological effects of ApoEr2 on synapse and dendritic spine formation-processes critical for learning and memory.<h4>Methodology/principal findings</h4>In a heterologous co-culture synapse assay, overexpression of ApoEr2 in COS7 cells significantly increased colocalization with synaptophysin in primary hippocampal neurons, suggesting that ApoEr2 promotes interaction with presynaptic structures. In primary neuronal cultures, overexpression of ApoEr2 increased dendritic spine density. Consistent with our in vitro findings, ApoEr2 knockout mice had decreased dendritic spine density in cortical layers II/III at 1 month of age. We also tested whether the interaction between ApoEr2 and its cytoplasmic adaptor proteins, specifically X11α and PSD-95, affected synapse and dendritic spine formation. X11α decreased cell surface levels of ApoEr2 along with synapse and dendritic spine density. In contrast, PSD-95 increased cell surface levels of ApoEr2 as well as synapse and dendritic spine density.<h4>Conclusions/significance</h4>These results suggest that ApoEr2 plays important roles in structure and function of CNS synapses and dendritic spines, and that these roles are modulated by cytoplasmic adaptor proteins X11α and PSD-95.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017203&type=printable |
| spellingShingle | Sonya B Dumanis Hyun-Jung Cha Jung Min Song Justin H Trotter Matthew Spitzer Ji-Yun Lee Edwin J Weeber R Scott Turner Daniel T S Pak G William Rebeck Hyang-Sook Hoe ApoE receptor 2 regulates synapse and dendritic spine formation. PLoS ONE |
| title | ApoE receptor 2 regulates synapse and dendritic spine formation. |
| title_full | ApoE receptor 2 regulates synapse and dendritic spine formation. |
| title_fullStr | ApoE receptor 2 regulates synapse and dendritic spine formation. |
| title_full_unstemmed | ApoE receptor 2 regulates synapse and dendritic spine formation. |
| title_short | ApoE receptor 2 regulates synapse and dendritic spine formation. |
| title_sort | apoe receptor 2 regulates synapse and dendritic spine formation |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017203&type=printable |
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