A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus

Rhizosphere microorganisms exert a significant influence in counteracting diverse external stresses and facilitating plant nutrient uptake. While certain rhizosphere microorganisms associated with Salix species have been investigated, numerous rhizosphere microorganisms from various Salix species re...

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Main Authors: Tianqing Feng, Juan Li, Xiaoning Mao, Xionglian Jin, Liang Cheng, Huichun Xie, Yonggui Ma
Format: Article
Language:English
Published: PeerJ Inc. 2025-03-01
Series:PeerJ
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Online Access:https://peerj.com/articles/19182.pdf
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author Tianqing Feng
Juan Li
Xiaoning Mao
Xionglian Jin
Liang Cheng
Huichun Xie
Yonggui Ma
author_facet Tianqing Feng
Juan Li
Xiaoning Mao
Xionglian Jin
Liang Cheng
Huichun Xie
Yonggui Ma
author_sort Tianqing Feng
collection DOAJ
description Rhizosphere microorganisms exert a significant influence in counteracting diverse external stresses and facilitating plant nutrient uptake. While certain rhizosphere microorganisms associated with Salix species have been investigated, numerous rhizosphere microorganisms from various Salix species remain underexplored. In this study, we employed high-throughput sequencing to examine the rhizosphere bacterial and fungal communities composition and diversity of three Salix species: Salix zangica (SZ), Salix myrtilllacea (SM), and Salix cheilophila (SC). Furthermore, the BugBase and FUNGuild were utilized to predict the functional roles of bacterial and fungal microorganisms. The findings revealed notable variations in the alpha and beta diversities of bacterial and fungal communities among the three Salix species exhibited significant differences (p < 0.05). The relative abundance of Flavobacterium was highest in the SZ samples, while Microvirga exhibited significant enrichment in the SM samples. Microvirga and Vishniacozyma demonstrate the highest number of nodes within their respective bacterial and fungal community network structures. The functions of bacterial microorganisms, including Gram-positive, potentially pathogenic, Gram-negative, and stress-tolerant types, exhibited significant variation among the three Salix species (p < 0.05). Furthermore, for the function of fungal microbe, the ectomycorrhizal guild had the highest abundance of symbiotic modes. This results demonstrated the critical role of ectomycorrhizal fungi in enhancing nutrient absorption and metabolism during the growth of Salix plants. Additionally, this findings also suggested that S. zangica plant was better well-suited for cultivation in stressful environments. These findings guide future questions about plant-microbe interactions, greatly enhancing our understanding of microbial communities for the healthy development of Salix plants.
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spelling doaj-art-7469aeaa2c564d5ea4e4d284d7ce63de2025-08-20T02:49:06ZengPeerJ Inc.PeerJ2167-83592025-03-0113e1918210.7717/peerj.19182A comparative analysis of the rhizosphere microbial communities among three species of the Salix genusTianqing Feng0Juan Li1Xiaoning Mao2Xionglian Jin3Liang Cheng4Huichun Xie5Yonggui Ma6Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Xining, ChinaQinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Xining, ChinaQinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Xining, ChinaKey Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province, Xining, ChinaRhizosphere microorganisms exert a significant influence in counteracting diverse external stresses and facilitating plant nutrient uptake. While certain rhizosphere microorganisms associated with Salix species have been investigated, numerous rhizosphere microorganisms from various Salix species remain underexplored. In this study, we employed high-throughput sequencing to examine the rhizosphere bacterial and fungal communities composition and diversity of three Salix species: Salix zangica (SZ), Salix myrtilllacea (SM), and Salix cheilophila (SC). Furthermore, the BugBase and FUNGuild were utilized to predict the functional roles of bacterial and fungal microorganisms. The findings revealed notable variations in the alpha and beta diversities of bacterial and fungal communities among the three Salix species exhibited significant differences (p < 0.05). The relative abundance of Flavobacterium was highest in the SZ samples, while Microvirga exhibited significant enrichment in the SM samples. Microvirga and Vishniacozyma demonstrate the highest number of nodes within their respective bacterial and fungal community network structures. The functions of bacterial microorganisms, including Gram-positive, potentially pathogenic, Gram-negative, and stress-tolerant types, exhibited significant variation among the three Salix species (p < 0.05). Furthermore, for the function of fungal microbe, the ectomycorrhizal guild had the highest abundance of symbiotic modes. This results demonstrated the critical role of ectomycorrhizal fungi in enhancing nutrient absorption and metabolism during the growth of Salix plants. Additionally, this findings also suggested that S. zangica plant was better well-suited for cultivation in stressful environments. These findings guide future questions about plant-microbe interactions, greatly enhancing our understanding of microbial communities for the healthy development of Salix plants.https://peerj.com/articles/19182.pdfSalixRhizosphereMicrobial communityDiversityNetworkFunction
spellingShingle Tianqing Feng
Juan Li
Xiaoning Mao
Xionglian Jin
Liang Cheng
Huichun Xie
Yonggui Ma
A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
PeerJ
Salix
Rhizosphere
Microbial community
Diversity
Network
Function
title A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
title_full A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
title_fullStr A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
title_full_unstemmed A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
title_short A comparative analysis of the rhizosphere microbial communities among three species of the Salix genus
title_sort comparative analysis of the rhizosphere microbial communities among three species of the salix genus
topic Salix
Rhizosphere
Microbial community
Diversity
Network
Function
url https://peerj.com/articles/19182.pdf
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