Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts
(1) Introduction: Polycaprolactone (PCL) materials have been developed with components that promote bone growth. The main objective of this work was to evaluate the biocompatibility and cytotoxic effects that different combinations of PCL with nanohydroxyapatite and strontium could produce on period...
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MDPI AG
2025-07-01
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| author | Ildefonso Serrano-Belmonte Javier Montero Ángel Román-Maciá Virginia Pérez-Fernández Luis M. Rodríguez-Lorenzo Nieves Cubo-Mateo Angélica Lara-Nuñez Ascensión Martínez-Cánovas |
| author_facet | Ildefonso Serrano-Belmonte Javier Montero Ángel Román-Maciá Virginia Pérez-Fernández Luis M. Rodríguez-Lorenzo Nieves Cubo-Mateo Angélica Lara-Nuñez Ascensión Martínez-Cánovas |
| author_sort | Ildefonso Serrano-Belmonte |
| collection | DOAJ |
| description | (1) Introduction: Polycaprolactone (PCL) materials have been developed with components that promote bone growth. The main objective of this work was to evaluate the biocompatibility and cytotoxic effects that different combinations of PCL with nanohydroxyapatite and strontium could produce on periodontal ligament stem cells (PDLSC). (2) Materials and Methods: PDLSCs were seeded in six 96-well plates. Three plates were used for the MTT test, and three were used for the Hoechst 33342 test. In each of the plates, three samples of different concentrations of PCL were introduced (PCL 100%, PCL 95% combined with nanohydroxyapatite functionalized with strontium, and PCL 90% with nanohydroxyapatite). Apoptosis was analyzed using Hoechst and cell viability combined with MTT at 24, 48, and 72 h. (3) Results: No statistically significant differences (<i>p</i> < 0.05) were found between the different concentrations of PCL or regarding the duration for which the cells were subjected to elution. (4) Conclusions: Pure PCL and both PCL combined with nanohydroxyapatite/strontium and nanohydroxyapatite are biocompatible materials, and there are no significant differences between them after apoptosis and in cell viability assays. |
| format | Article |
| id | doaj-art-74687daec29c4e5ab022ea74408056b7 |
| institution | DOAJ |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-74687daec29c4e5ab022ea74408056b72025-08-20T03:16:42ZengMDPI AGApplied Sciences2076-34172025-07-011513754010.3390/app15137540Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone GraftsIldefonso Serrano-Belmonte0Javier Montero1Ángel Román-Maciá2Virginia Pérez-Fernández3Luis M. Rodríguez-Lorenzo4Nieves Cubo-Mateo5Angélica Lara-Nuñez6Ascensión Martínez-Cánovas7School of Dentistry, Faculty of Medicine, Clínica Odontológica Universitaria, Hospital Morales Meseguer, University of Murcia, Avda. Marqués de los Vélez s/n, 30008 Murcia, SpainDepartment of Surgery, Faculty of Medicine, University of Salamanca, 37008 Salamanca, SpainSchool of Dentistry, Faculty of Medicine, Clínica Odontológica Universitaria, Hospital Morales Meseguer, University of Murcia, Avda. Marqués de los Vélez s/n, 30008 Murcia, SpainDepartamento de Ciencias Sociosanitarias, Institute of Biomedical Research (IMIB-Arrixaca), University of Murcia, 30120 Murcia, SpainICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, SpainGrupo de Investigación ARIES, Universidad Nebrija, Calle de Santa Cruz de Marcenado 27, 28015 Madrid, SpainICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, SpainSchool of Dentistry, Faculty of Medicine, Clínica Odontológica Universitaria, Hospital Morales Meseguer, University of Murcia, Avda. Marqués de los Vélez s/n, 30008 Murcia, Spain(1) Introduction: Polycaprolactone (PCL) materials have been developed with components that promote bone growth. The main objective of this work was to evaluate the biocompatibility and cytotoxic effects that different combinations of PCL with nanohydroxyapatite and strontium could produce on periodontal ligament stem cells (PDLSC). (2) Materials and Methods: PDLSCs were seeded in six 96-well plates. Three plates were used for the MTT test, and three were used for the Hoechst 33342 test. In each of the plates, three samples of different concentrations of PCL were introduced (PCL 100%, PCL 95% combined with nanohydroxyapatite functionalized with strontium, and PCL 90% with nanohydroxyapatite). Apoptosis was analyzed using Hoechst and cell viability combined with MTT at 24, 48, and 72 h. (3) Results: No statistically significant differences (<i>p</i> < 0.05) were found between the different concentrations of PCL or regarding the duration for which the cells were subjected to elution. (4) Conclusions: Pure PCL and both PCL combined with nanohydroxyapatite/strontium and nanohydroxyapatite are biocompatible materials, and there are no significant differences between them after apoptosis and in cell viability assays.https://www.mdpi.com/2076-3417/15/13/7540polycaprolactonebone regenerationperiodontal ligamentbiocompatibilitycytotoxicity |
| spellingShingle | Ildefonso Serrano-Belmonte Javier Montero Ángel Román-Maciá Virginia Pérez-Fernández Luis M. Rodríguez-Lorenzo Nieves Cubo-Mateo Angélica Lara-Nuñez Ascensión Martínez-Cánovas Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts Applied Sciences polycaprolactone bone regeneration periodontal ligament biocompatibility cytotoxicity |
| title | Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts |
| title_full | Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts |
| title_fullStr | Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts |
| title_full_unstemmed | Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts |
| title_short | Preclinical Performance of Customized Polycaprolactone-Based Hybrid Bone Grafts |
| title_sort | preclinical performance of customized polycaprolactone based hybrid bone grafts |
| topic | polycaprolactone bone regeneration periodontal ligament biocompatibility cytotoxicity |
| url | https://www.mdpi.com/2076-3417/15/13/7540 |
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