Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma
Objective. The intrahepatic stem cells, also known as hepatic progenitor cells (HPCs), are able to differentiate into hepatocytes and bile duct epithelia. By exposure of different injuries and different hepatocarcinogenic regimens, the mature hepatocytes can no longer effectively regenerate; stem ce...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/145253 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551956162281472 |
|---|---|
| author | Shu-Qin Jia Jian-Jun Ren Pei-De Dong Xing-Kai Meng |
| author_facet | Shu-Qin Jia Jian-Jun Ren Pei-De Dong Xing-Kai Meng |
| author_sort | Shu-Qin Jia |
| collection | DOAJ |
| description | Objective. The intrahepatic stem cells, also known as hepatic progenitor cells (HPCs), are able to differentiate into hepatocytes and bile duct epithelia. By exposure of different injuries and different hepatocarcinogenic regimens, the mature hepatocytes can no longer effectively regenerate; stem cells are involved in the pathogenesis of hepatocellular carcinoma. Methods. Immunohistochemistry was performed on 107 paraffin-embedded hepatocellular carcinoma specimens with the marker of hepatocyte and hepatocellular carcinoma (HepPar1), biliary differentiation (CK7,CK19), haemopoietic stem cell (HSC) (c-kit/CD117, CD34, and Thy-1/CD90), HPC specific markers (OV-6), and Ki-67, p53 protein. Results. HPCs can be identified in the tumor nodules, around the edge of tumor nodules, and in the portal tracts of the paracirrhosis nodules being positive in HepPar1, CK7, CK19, and OV-6, but they failed to immunostain with CD117, CD34, and CD90. The HPCs positive in Ki-67 are observed in the tumor and paracirrhosis tissues. In 107 specimens, 40.2% (43/107) HCC tissues expressed p53 protein, lower than that of the HPCs around the tumor nodules (46.7%, 50/107) and much higher than that of the HPCs around the paracirrhosis nodules (8.41%, 9/107). Conclusion. Human hepatocellular carcinogenesis may be based on transformation of HPCs, not HSCs, through the formation of the transitional cells (hepatocyte-like cells and bile ductal cells). |
| format | Article |
| id | doaj-art-745f10f269c94296bb59e352a8327e55 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-745f10f269c94296bb59e352a8327e552025-02-03T05:59:57ZengWileyGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/145253145253Probing the Hepatic Progenitor Cell in Human Hepatocellular CarcinomaShu-Qin Jia0Jian-Jun Ren1Pei-De Dong2Xing-Kai Meng3Surgery Laboratory, The Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, ChinaDepartment of Surgery, The Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, ChinaDepartment of Surgery, The Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, ChinaDepartment of Surgery, The Affiliated Hospital, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, ChinaObjective. The intrahepatic stem cells, also known as hepatic progenitor cells (HPCs), are able to differentiate into hepatocytes and bile duct epithelia. By exposure of different injuries and different hepatocarcinogenic regimens, the mature hepatocytes can no longer effectively regenerate; stem cells are involved in the pathogenesis of hepatocellular carcinoma. Methods. Immunohistochemistry was performed on 107 paraffin-embedded hepatocellular carcinoma specimens with the marker of hepatocyte and hepatocellular carcinoma (HepPar1), biliary differentiation (CK7,CK19), haemopoietic stem cell (HSC) (c-kit/CD117, CD34, and Thy-1/CD90), HPC specific markers (OV-6), and Ki-67, p53 protein. Results. HPCs can be identified in the tumor nodules, around the edge of tumor nodules, and in the portal tracts of the paracirrhosis nodules being positive in HepPar1, CK7, CK19, and OV-6, but they failed to immunostain with CD117, CD34, and CD90. The HPCs positive in Ki-67 are observed in the tumor and paracirrhosis tissues. In 107 specimens, 40.2% (43/107) HCC tissues expressed p53 protein, lower than that of the HPCs around the tumor nodules (46.7%, 50/107) and much higher than that of the HPCs around the paracirrhosis nodules (8.41%, 9/107). Conclusion. Human hepatocellular carcinogenesis may be based on transformation of HPCs, not HSCs, through the formation of the transitional cells (hepatocyte-like cells and bile ductal cells).http://dx.doi.org/10.1155/2013/145253 |
| spellingShingle | Shu-Qin Jia Jian-Jun Ren Pei-De Dong Xing-Kai Meng Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma Gastroenterology Research and Practice |
| title | Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma |
| title_full | Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma |
| title_fullStr | Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma |
| title_full_unstemmed | Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma |
| title_short | Probing the Hepatic Progenitor Cell in Human Hepatocellular Carcinoma |
| title_sort | probing the hepatic progenitor cell in human hepatocellular carcinoma |
| url | http://dx.doi.org/10.1155/2013/145253 |
| work_keys_str_mv | AT shuqinjia probingthehepaticprogenitorcellinhumanhepatocellularcarcinoma AT jianjunren probingthehepaticprogenitorcellinhumanhepatocellularcarcinoma AT peidedong probingthehepaticprogenitorcellinhumanhepatocellularcarcinoma AT xingkaimeng probingthehepaticprogenitorcellinhumanhepatocellularcarcinoma |