A screening setup to streamline in vitro engineered living material cultures with the host
Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. Determining the conditions that sustain ELM performance while ensuring compatibility with ELM hosts i...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004988 |
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| author | Krupansh Desai Shrikrishnan Sankaran Aránzazu del Campo Sara Trujillo |
| author_facet | Krupansh Desai Shrikrishnan Sankaran Aránzazu del Campo Sara Trujillo |
| author_sort | Krupansh Desai |
| collection | DOAJ |
| description | Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. Determining the conditions that sustain ELM performance while ensuring compatibility with ELM hosts is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through in vitro investigations. Currently, there are no standards that ensure ELM compatibility with host tissues. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential in vitro. We showed proliferation of three bacterial species encapsulated in hydrogels over time and screened six different cell culture media. We fabricated ELMs in bilayer and monolayer formats and tracked bacterial leakage as a measure of ELM biocontainment. After screening, an appropriate medium was selected that sustained growth of an ELM, and it was used to study cytocompatibility in vitro. ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. Our work illustrates a simple setup to streamline the screening of compatible environmental conditions of ELMs with the host. |
| format | Article |
| id | doaj-art-74489146844340309c87ad0a12ad1e0f |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-74489146844340309c87ad0a12ad1e0f2025-01-17T04:52:13ZengElsevierMaterials Today Bio2590-00642025-02-0130101437A screening setup to streamline in vitro engineered living material cultures with the hostKrupansh Desai0Shrikrishnan Sankaran1Aránzazu del Campo2Sara Trujillo3INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, Germany; Chemistry Department, Saarland University, Saarbrücken, 66123, GermanyINM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, GermanyINM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, Germany; Chemistry Department, Saarland University, Saarbrücken, 66123, GermanyINM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, Germany; Saarland University, Pharma Science Hub (PSH), 66123 Saarbrucken, Germany; Corresponding author. INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, Germany.Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. Determining the conditions that sustain ELM performance while ensuring compatibility with ELM hosts is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through in vitro investigations. Currently, there are no standards that ensure ELM compatibility with host tissues. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential in vitro. We showed proliferation of three bacterial species encapsulated in hydrogels over time and screened six different cell culture media. We fabricated ELMs in bilayer and monolayer formats and tracked bacterial leakage as a measure of ELM biocontainment. After screening, an appropriate medium was selected that sustained growth of an ELM, and it was used to study cytocompatibility in vitro. ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. Our work illustrates a simple setup to streamline the screening of compatible environmental conditions of ELMs with the host.http://www.sciencedirect.com/science/article/pii/S2590006424004988Engineered living materialsLiving therapeuticsBiocompatibilityIn vitro culture |
| spellingShingle | Krupansh Desai Shrikrishnan Sankaran Aránzazu del Campo Sara Trujillo A screening setup to streamline in vitro engineered living material cultures with the host Materials Today Bio Engineered living materials Living therapeutics Biocompatibility In vitro culture |
| title | A screening setup to streamline in vitro engineered living material cultures with the host |
| title_full | A screening setup to streamline in vitro engineered living material cultures with the host |
| title_fullStr | A screening setup to streamline in vitro engineered living material cultures with the host |
| title_full_unstemmed | A screening setup to streamline in vitro engineered living material cultures with the host |
| title_short | A screening setup to streamline in vitro engineered living material cultures with the host |
| title_sort | screening setup to streamline in vitro engineered living material cultures with the host |
| topic | Engineered living materials Living therapeutics Biocompatibility In vitro culture |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004988 |
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