Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum
Background: The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide. Objectives: Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcu...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524001723 |
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| author | Ning Xie Tengfei Ma Yuan Gao Jiani Fu Chune Tan Zheng Wang Zhuo Wang Henrike Krüger-Haker Andrea T. Feßler Congming Wu Jianzhong Shen Stefan Schwarz Yang Wang |
| author_facet | Ning Xie Tengfei Ma Yuan Gao Jiani Fu Chune Tan Zheng Wang Zhuo Wang Henrike Krüger-Haker Andrea T. Feßler Congming Wu Jianzhong Shen Stefan Schwarz Yang Wang |
| author_sort | Ning Xie |
| collection | DOAJ |
| description | Background: The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide. Objectives: Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcus equorum isolate harbouring two distinct cfr-carrying plasmids. Methods: Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. De novo hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools. Results: Staphylococcus equorum isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two cfr-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported cfr-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene lsa(B). In addition, plasmid pSN42–51 K carried two erm(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene ant(6)-Ia as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene hmtp. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the cfr-carrying plasmids. Conclusion: This is the first report of S. equorum carrying two distinct cfr-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health. |
| format | Article |
| id | doaj-art-742e00fab280460389e1e070e327a171 |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-742e00fab280460389e1e070e327a1712025-08-20T01:59:47ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-013917017410.1016/j.jgar.2024.09.004Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorumNing Xie0Tengfei Ma1Yuan Gao2Jiani Fu3Chune Tan4Zheng Wang5Zhuo Wang6Henrike Krüger-Haker7Andrea T. Feßler8Congming Wu9Jianzhong Shen10Stefan Schwarz11Yang Wang12Technology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaVito Diagnostic Technology Company Limited, Hangzhou, Zhejiang, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaCollege of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaCenter for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Institute of Microbiology and Epizootics, Berlin, Germany; Veterinary Centre for Resistance Research, School of Veterinary Medicine, Freie Universität Berlin, Berlin, GermanyCenter for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Institute of Microbiology and Epizootics, Berlin, Germany; Veterinary Centre for Resistance Research, School of Veterinary Medicine, Freie Universität Berlin, Berlin, GermanyTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China; College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei, China; Center for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Institute of Microbiology and Epizootics, Berlin, GermanyTechnology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya, Hainan, China; National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China; Corresponding author.Background: The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide. Objectives: Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcus equorum isolate harbouring two distinct cfr-carrying plasmids. Methods: Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. De novo hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools. Results: Staphylococcus equorum isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two cfr-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported cfr-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene lsa(B). In addition, plasmid pSN42–51 K carried two erm(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene ant(6)-Ia as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene hmtp. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the cfr-carrying plasmids. Conclusion: This is the first report of S. equorum carrying two distinct cfr-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.http://www.sciencedirect.com/science/article/pii/S2213716524001723Staphylococcus equorumTwo distinct cfr-carrying plasmidsHeavy metal resistance genes |
| spellingShingle | Ning Xie Tengfei Ma Yuan Gao Jiani Fu Chune Tan Zheng Wang Zhuo Wang Henrike Krüger-Haker Andrea T. Feßler Congming Wu Jianzhong Shen Stefan Schwarz Yang Wang Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum Journal of Global Antimicrobial Resistance Staphylococcus equorum Two distinct cfr-carrying plasmids Heavy metal resistance genes |
| title | Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum |
| title_full | Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum |
| title_fullStr | Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum |
| title_full_unstemmed | Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum |
| title_short | Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum |
| title_sort | two novel plasmids harbouring the multiresistance gene cfr in porcine staphylococcus equorum |
| topic | Staphylococcus equorum Two distinct cfr-carrying plasmids Heavy metal resistance genes |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524001723 |
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