Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2
Background. One of the main problems of antibiotic therapy is the development of resistance in microorganisms. In this regard, the search for new natural antibiotics, including those of plant origin, is relevant. The aim of the study was the determination of antibacterial action mechanisms of aqueou...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2023-12-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/1058 |
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| author | A. Кh. Baymiev A. К. Chuvatkina A. A. Vladimirova R. Т. Matniyazov А. R. Mavzyutov А. Kh. Baymiev |
| author_facet | A. Кh. Baymiev A. К. Chuvatkina A. A. Vladimirova R. Т. Matniyazov А. R. Mavzyutov А. Kh. Baymiev |
| author_sort | A. Кh. Baymiev |
| collection | DOAJ |
| description | Background. One of the main problems of antibiotic therapy is the development of resistance in microorganisms. In this regard, the search for new natural antibiotics, including those of plant origin, is relevant. The aim of the study was the determination of antibacterial action mechanisms of aqueous and alcoholic extracts of medicinal plants collected on the territory of the Republic of Bashkortostan. Methods. The bark, roots, stems, leaves, and inflorescences of medicinal plants were selected as objects of the study. The mechanisms of antibacterial action of plant preparations were determined via high throughput screening using the Dualrep2 double reporter system. Results. The mechanisms of the antibacterial action of certain plant extracts obtained from fresh and dried raw materials were established. It has been shown that the products of alcohol extraction of yarrow inflorescences inhibit protein synthesis, while the products of celandine roots and stems cause the activation of the SOS DNA repair system in Escherichia coli bacteria. Conclusion. The results obtained allow us to consider the studied extracts of medicinal plants as a basis for obtaining new antibacterial agents with a specific mechanism of action. |
| format | Article |
| id | doaj-art-7416431c76214cabb7ca249d293cf63e |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2023-12-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-7416431c76214cabb7ca249d293cf63e2025-08-20T03:59:08ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902023-12-01687-8111610.37489/0235-2990-2023-68-7-8-11-16974Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2A. Кh. Baymiev0A. К. Chuvatkina1A. A. Vladimirova2R. Т. Matniyazov3А. R. Mavzyutov4А. Kh. Baymiev5Institute of Biochemistry and Genetics; Bashkir State Medical University of the Ministry of Health of the Russian FederationBashkir State Medical University of the Ministry of Health of the Russian FederationInstitute of Biochemistry and Genetics, Ufa Federal Research Centre of the Russian Academy of SciencesInstitute of Biochemistry and Genetics, Ufa Federal Research Centre of the Russian Academy of SciencesBashkir State Medical University of the Ministry of Health of the Russian FederationInstitute of Biochemistry and Genetics, Ufa Federal Research Centre of the Russian Academy of Sciences; Bashkir State Medical University of the Ministry of Health of the Russian FederationBackground. One of the main problems of antibiotic therapy is the development of resistance in microorganisms. In this regard, the search for new natural antibiotics, including those of plant origin, is relevant. The aim of the study was the determination of antibacterial action mechanisms of aqueous and alcoholic extracts of medicinal plants collected on the territory of the Republic of Bashkortostan. Methods. The bark, roots, stems, leaves, and inflorescences of medicinal plants were selected as objects of the study. The mechanisms of antibacterial action of plant preparations were determined via high throughput screening using the Dualrep2 double reporter system. Results. The mechanisms of the antibacterial action of certain plant extracts obtained from fresh and dried raw materials were established. It has been shown that the products of alcohol extraction of yarrow inflorescences inhibit protein synthesis, while the products of celandine roots and stems cause the activation of the SOS DNA repair system in Escherichia coli bacteria. Conclusion. The results obtained allow us to consider the studied extracts of medicinal plants as a basis for obtaining new antibacterial agents with a specific mechanism of action.https://www.antibiotics-chemotherapy.ru/jour/article/view/1058antibacterial mechanismmedicinal plantsreporters |
| spellingShingle | A. Кh. Baymiev A. К. Chuvatkina A. A. Vladimirova R. Т. Matniyazov А. R. Mavzyutov А. Kh. Baymiev Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 Антибиотики и Химиотерапия antibacterial mechanism medicinal plants reporters |
| title | Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 |
| title_full | Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 |
| title_fullStr | Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 |
| title_full_unstemmed | Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 |
| title_short | Analysis of Antibacterial Action Mechanisms of Medicinal Plant Extracts Using Dual Reporter System Dualrep2 |
| title_sort | analysis of antibacterial action mechanisms of medicinal plant extracts using dual reporter system dualrep2 |
| topic | antibacterial mechanism medicinal plants reporters |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/1058 |
| work_keys_str_mv | AT akhbaymiev analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 AT akchuvatkina analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 AT aavladimirova analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 AT rtmatniyazov analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 AT armavzyutov analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 AT akhbaymiev analysisofantibacterialactionmechanismsofmedicinalplantextractsusingdualreportersystemdualrep2 |