Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology
Objective To analyze the changes of gut microbiota in the progression of alcoholic fatty liver disease(AFLD) using 16S rDNA sequencing technology, and to explore the possible mechanism of AFLD progression in mice. Methods A total of 60 male C57BL/6 mice were randomly divided into control group (n=35...
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Editorial Office of Journal of Guangxi Medical University
2024-08-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.005 |
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| author | DONG Xiaowei WU Changliang WANG Zhenchang QIN Suping HE Jianke HUANG Huiyi |
| author_facet | DONG Xiaowei WU Changliang WANG Zhenchang QIN Suping HE Jianke HUANG Huiyi |
| author_sort | DONG Xiaowei |
| collection | DOAJ |
| description | Objective To analyze the changes of gut microbiota in the progression of alcoholic fatty liver disease(AFLD) using 16S rDNA sequencing technology, and to explore the possible mechanism of AFLD progression in mice. Methods A total of 60 male C57BL/6 mice were randomly divided into control group (n=35) and model group (n=25). After adaptive feeding for 5 days, mice in the control group were fed a control Lieber-DeCarli fluid feeds (TP4030C) daily, and mice in the model group were fed Lieber-DeCarli fluid feeds (TP4030B) containing 4% ethanol daily. After 30 consecutive days, hematoxylin-eosin (HE) staining was used to observe the pathological changes of the liver. 16S rDNA sequencing was used to analyze the composition and structure of gut microbiota. Results Compared with the control group, Alpha diversity results of the gut microbiota of mice in the AFLD model group showed reduced species richness (P < 0.05). Beta diversity results indicated significant differences in the structural composition and abundance of the gut microbiota of the two groups of mice (P < 0.05). Compared with the control group, the relative abundance of Faecalibaculum, Dubosiella, Allobaculum, Ruminococcaceae UCG-013 were higher in the model group, while the relative abundance of Lactobacillus and Lachnospiraceae NK4A136 group were lower in the model group. The activity of gut microbiota in alanyltransferase, glutathione hydrolase and histone acetyltransferase pathways was significantly increased in the model group of mice (P < 0.05). Conclusion AFLD C57BL/6 mouse model is successfully constructed on Lieber-DeCarli fluid feeds containing 4% ethanol, and both the structure and composition of the gut microbiota of AFLD mice are altered. Alcohol may accelerate the progression of AFLD by disrupting gut microbial homeostasis and increasing the activities of alanyltransferase, glutathione hydrolase, and histone acetyltransferase pathways. |
| format | Article |
| id | doaj-art-7403dbea8cfa4c2494eb17f447ec5961 |
| institution | OA Journals |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-08-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
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| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-7403dbea8cfa4c2494eb17f447ec59612025-08-20T02:15:20ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-08-014181134114010.16190/j.cnki.45-1211/r.2024.08.005gxykdxxb-41-8-1134Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technologyDONG Xiaowei0WU Changliang1WANG Zhenchang2QIN Suping3HE Jianke4HUANG Huiyi5Guangxi University of Chinese Medicine, Nanning 530200, ChinaGuangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530201, ChinaGuangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530201, ChinaGuangxi University of Chinese Medicine, Nanning 530200, ChinaGuangxi University of Chinese Medicine, Nanning 530200, ChinaGuangxi University of Chinese Medicine, Nanning 530200, ChinaObjective To analyze the changes of gut microbiota in the progression of alcoholic fatty liver disease(AFLD) using 16S rDNA sequencing technology, and to explore the possible mechanism of AFLD progression in mice. Methods A total of 60 male C57BL/6 mice were randomly divided into control group (n=35) and model group (n=25). After adaptive feeding for 5 days, mice in the control group were fed a control Lieber-DeCarli fluid feeds (TP4030C) daily, and mice in the model group were fed Lieber-DeCarli fluid feeds (TP4030B) containing 4% ethanol daily. After 30 consecutive days, hematoxylin-eosin (HE) staining was used to observe the pathological changes of the liver. 16S rDNA sequencing was used to analyze the composition and structure of gut microbiota. Results Compared with the control group, Alpha diversity results of the gut microbiota of mice in the AFLD model group showed reduced species richness (P < 0.05). Beta diversity results indicated significant differences in the structural composition and abundance of the gut microbiota of the two groups of mice (P < 0.05). Compared with the control group, the relative abundance of Faecalibaculum, Dubosiella, Allobaculum, Ruminococcaceae UCG-013 were higher in the model group, while the relative abundance of Lactobacillus and Lachnospiraceae NK4A136 group were lower in the model group. The activity of gut microbiota in alanyltransferase, glutathione hydrolase and histone acetyltransferase pathways was significantly increased in the model group of mice (P < 0.05). Conclusion AFLD C57BL/6 mouse model is successfully constructed on Lieber-DeCarli fluid feeds containing 4% ethanol, and both the structure and composition of the gut microbiota of AFLD mice are altered. Alcohol may accelerate the progression of AFLD by disrupting gut microbial homeostasis and increasing the activities of alanyltransferase, glutathione hydrolase, and histone acetyltransferase pathways.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.005alcoholic fatty liver disease16s rrna gene sequencinggut microbiotadominant bacteria |
| spellingShingle | DONG Xiaowei WU Changliang WANG Zhenchang QIN Suping HE Jianke HUANG Huiyi Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology Guangxi Yike Daxue xuebao alcoholic fatty liver disease 16s rrna gene sequencing gut microbiota dominant bacteria |
| title | Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology |
| title_full | Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology |
| title_fullStr | Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology |
| title_full_unstemmed | Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology |
| title_short | Analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16S rDNA sequencing technology |
| title_sort | analysis of gut microbiota changes in alcoholic fatty liver disease in progression based on 16s rdna sequencing technology |
| topic | alcoholic fatty liver disease 16s rrna gene sequencing gut microbiota dominant bacteria |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.005 |
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