Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity
Abstract Viruses are responsible for numerous serious outbreaks and pandemics worldwide. In this context, lectins, which are carbohydrate-binding proteins, have garnered attention due to their antiviral properties against RNA and DNA viruses. The antiviral potential of the purified and well-characte...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-09967-z |
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| author | Yousra A. El-Maradny Marwa M. Abu-Serie Mona H. Hashish Heba S. Selim Abdulrahman M. Saleh Esmail M. El-Fakharany |
| author_facet | Yousra A. El-Maradny Marwa M. Abu-Serie Mona H. Hashish Heba S. Selim Abdulrahman M. Saleh Esmail M. El-Fakharany |
| author_sort | Yousra A. El-Maradny |
| collection | DOAJ |
| description | Abstract Viruses are responsible for numerous serious outbreaks and pandemics worldwide. In this context, lectins, which are carbohydrate-binding proteins, have garnered attention due to their antiviral properties against RNA and DNA viruses. The antiviral potential of the purified and well-characterized lectin from Pleurotus ostreatus (POL) was assessed against various viruses. POL showed potent antiviral activity against HCV with IC50 values of 68.75 nM and 52.13 nM for determining the blocking and neutralizing infectivity, respectively. POL exhibited IC50 values of 42.75 and 14.88 nM against HBV through treatment and blocking mechanisms, respectively. Notably, POL demonstrated a 58.80% binding capacity to cell-surface CD81, while the IC50 values for targeting the scavenger receptor class B-type I (SR-B1), HCV-NS3/4A protease inhibition, and anti-HBV polymerase activity were established at 10.08, 10.98, and 4.22 nM, respectively. The outcomes of this investigation offer crucial insights into the mechanisms through which POL effectively inhibits infection caused by different viruses. These findings have the potential to inform the development of antiviral strategies and therapeutic interventions. |
| format | Article |
| id | doaj-art-73eb9a4960644c07b82f4a2ebc544ece |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-73eb9a4960644c07b82f4a2ebc544ece2025-08-20T04:02:46ZengNature PortfolioScientific Reports2045-23222025-07-0115111610.1038/s41598-025-09967-zBiochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activityYousra A. El-Maradny0Marwa M. Abu-Serie1Mona H. Hashish2Heba S. Selim3Abdulrahman M. Saleh4Esmail M. El-Fakharany5Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg AL-ArabDepartment of Medical Biotechnology, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg AL-ArabMicrobiology Department, High Institute of Public Health, Alexandria UniversityMicrobiology Department, High Institute of Public Health, Alexandria UniversityDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini StreetProtein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg AL-ArabAbstract Viruses are responsible for numerous serious outbreaks and pandemics worldwide. In this context, lectins, which are carbohydrate-binding proteins, have garnered attention due to their antiviral properties against RNA and DNA viruses. The antiviral potential of the purified and well-characterized lectin from Pleurotus ostreatus (POL) was assessed against various viruses. POL showed potent antiviral activity against HCV with IC50 values of 68.75 nM and 52.13 nM for determining the blocking and neutralizing infectivity, respectively. POL exhibited IC50 values of 42.75 and 14.88 nM against HBV through treatment and blocking mechanisms, respectively. Notably, POL demonstrated a 58.80% binding capacity to cell-surface CD81, while the IC50 values for targeting the scavenger receptor class B-type I (SR-B1), HCV-NS3/4A protease inhibition, and anti-HBV polymerase activity were established at 10.08, 10.98, and 4.22 nM, respectively. The outcomes of this investigation offer crucial insights into the mechanisms through which POL effectively inhibits infection caused by different viruses. These findings have the potential to inform the development of antiviral strategies and therapeutic interventions.https://doi.org/10.1038/s41598-025-09967-zMushroomsLectinsAntiviralHCVHBV |
| spellingShingle | Yousra A. El-Maradny Marwa M. Abu-Serie Mona H. Hashish Heba S. Selim Abdulrahman M. Saleh Esmail M. El-Fakharany Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity Scientific Reports Mushrooms Lectins Antiviral HCV HBV |
| title | Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity |
| title_full | Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity |
| title_fullStr | Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity |
| title_full_unstemmed | Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity |
| title_short | Biochemical characterization of a novel purified lectin extracted from Pleurotus ostreatus mushroom for its antiviral activity |
| title_sort | biochemical characterization of a novel purified lectin extracted from pleurotus ostreatus mushroom for its antiviral activity |
| topic | Mushrooms Lectins Antiviral HCV HBV |
| url | https://doi.org/10.1038/s41598-025-09967-z |
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