Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)

<i>Spalangia endius</i> Walker, a pupal parasitoid of the alien invasive pest <i>Zeugodacus cucurbitae</i> (Coquillett), causes 33% host mortality. This study assessed whether combining <i>S. endius</i> with insecticides (abamectin, thiamethoxam, nitenpyram, emame...

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Main Authors: Lei Li, Dongyin Han, Jing Zhao, Haiyan Qiu, Fangping Zhang, Zhengpei Ye, Yueguan Fu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/16/7/716
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author Lei Li
Dongyin Han
Jing Zhao
Haiyan Qiu
Fangping Zhang
Zhengpei Ye
Yueguan Fu
author_facet Lei Li
Dongyin Han
Jing Zhao
Haiyan Qiu
Fangping Zhang
Zhengpei Ye
Yueguan Fu
author_sort Lei Li
collection DOAJ
description <i>Spalangia endius</i> Walker, a pupal parasitoid of the alien invasive pest <i>Zeugodacus cucurbitae</i> (Coquillett), causes 33% host mortality. This study assessed whether combining <i>S. endius</i> with insecticides (abamectin, thiamethoxam, nitenpyram, emamectin benzoate, or beta-cypermethrin)—all effective against <i>Z. cucurbitae</i>—could enhance control efficacy. Among these, abamectin was the least toxic to adult <i>S. endius</i>. Surface contact treatments with 12 and 15 mg a.i./kg of abamectin did not significantly increase <i>S. endius</i> mortality. However, mixing 12 mg a.i./kg of abamectin into a honey solution to encourage ingestion decreased the survival, parasitism, and fecundity of <i>S. endius</i>. In olfactometer assays, <i>S. endius</i> adults avoided abamectin-treated host pupae, though prior exposure to abamectin mitigated this avoidance. The timing of abamectin soil application relative to host pupation and <i>S. endius</i> release affects host mortality. The most effective timing is spraying abamectin before host pupation (to expose <i>Z. cucurbitae</i> larvae) and then releasing <i>S. endius</i>. Field trials confirmed that combining abamectin (12 mg a.i./kg) with <i>S. endius</i> increased host mortality more than either treatment alone. In conclusion, abamectin (12 mg a.i./kg) is a suitable insecticide for combination with <i>S. endius</i> to control <i>Z. cucurbitae.</i> The application sequence should be spraying abamectin before hosts pupate and, only after that, releasing the parasitoid.
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spelling doaj-art-73d3ace6d77c45caabeb7a0a4f1615c72025-08-20T03:08:09ZengMDPI AGInsects2075-44502025-07-0116771610.3390/insects16070716Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)Lei Li0Dongyin Han1Jing Zhao2Haiyan Qiu3Fangping Zhang4Zhengpei Ye5Yueguan Fu6Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Sciences, Haikou 571101, China<i>Spalangia endius</i> Walker, a pupal parasitoid of the alien invasive pest <i>Zeugodacus cucurbitae</i> (Coquillett), causes 33% host mortality. This study assessed whether combining <i>S. endius</i> with insecticides (abamectin, thiamethoxam, nitenpyram, emamectin benzoate, or beta-cypermethrin)—all effective against <i>Z. cucurbitae</i>—could enhance control efficacy. Among these, abamectin was the least toxic to adult <i>S. endius</i>. Surface contact treatments with 12 and 15 mg a.i./kg of abamectin did not significantly increase <i>S. endius</i> mortality. However, mixing 12 mg a.i./kg of abamectin into a honey solution to encourage ingestion decreased the survival, parasitism, and fecundity of <i>S. endius</i>. In olfactometer assays, <i>S. endius</i> adults avoided abamectin-treated host pupae, though prior exposure to abamectin mitigated this avoidance. The timing of abamectin soil application relative to host pupation and <i>S. endius</i> release affects host mortality. The most effective timing is spraying abamectin before host pupation (to expose <i>Z. cucurbitae</i> larvae) and then releasing <i>S. endius</i>. Field trials confirmed that combining abamectin (12 mg a.i./kg) with <i>S. endius</i> increased host mortality more than either treatment alone. In conclusion, abamectin (12 mg a.i./kg) is a suitable insecticide for combination with <i>S. endius</i> to control <i>Z. cucurbitae.</i> The application sequence should be spraying abamectin before hosts pupate and, only after that, releasing the parasitoid.https://www.mdpi.com/2075-4450/16/7/716melon flypupal parasitoidabamectintoxicity
spellingShingle Lei Li
Dongyin Han
Jing Zhao
Haiyan Qiu
Fangping Zhang
Zhengpei Ye
Yueguan Fu
Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
Insects
melon fly
pupal parasitoid
abamectin
toxicity
title Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
title_full Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
title_fullStr Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
title_full_unstemmed Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
title_short Combination of the Parasitoid <i>Spalangia endius</i> Walker and Chemical Pesticides for the Control of <i>Zeugodacus cucurbitae</i> (Coquillett)
title_sort combination of the parasitoid i spalangia endius i walker and chemical pesticides for the control of i zeugodacus cucurbitae i coquillett
topic melon fly
pupal parasitoid
abamectin
toxicity
url https://www.mdpi.com/2075-4450/16/7/716
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