Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions
Bletilla striata is a terrestrial sympodial orchid. Substrates used for outdoor growing, differing in the mixture of added components and nutrients, were chosen for acclimatization of the asymbiotic propagated plants. A total of 651 Bletilla striata orchids were planted in 3 commercial substrates: ″...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2012-05-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/14524 |
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| author | Helena LESAR Barbara HLEBEC Nataša ČERANIČ Damijana KASTELEC Zlata LUTHAR |
| author_facet | Helena LESAR Barbara HLEBEC Nataša ČERANIČ Damijana KASTELEC Zlata LUTHAR |
| author_sort | Helena LESAR |
| collection | DOAJ |
| description | Bletilla striata is a terrestrial sympodial orchid. Substrates used for outdoor growing, differing in the mixture of added components and nutrients, were chosen for acclimatization of the asymbiotic propagated plants. A total of 651 Bletilla striata orchids were planted in 3 commercial substrates: ″Tonsubstrat″ (Ton), ″Baltisches substrat″ (Baltski) and ″Royal-Garden″ (Royal). Prior to acclimatization, the plants were 2.5 cm on average, with at least 2 leaves and 2 - 3 cm long root or roots. Fewest plants (3.1%) died in Ton substrate, 3.2% in Baltski substrate and 5.0% in Royal substrate. There were no statistically significant differences among substrates (p = 0.558) in the percentage of plants that died. Substrates used in combination with the chosen phenophase and established conditions were suitable for acclimatization of Bletilla striata orchids, whereby 95-97% of plants successfully adapted from heterotrophic to autotrophic conditions in a very short period of two months. The basic conditions for success are that plants are large and vital enough prior to acclimatization, that the substrate is appropriate and that appropriate conditions of relative humidity, temperature, light and ventilation without major fluctuations of these factors are ensured during acclimatization. |
| format | Article |
| id | doaj-art-73d2e7fb94a646f5bd8defa38508ec9f |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
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| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-73d2e7fb94a646f5bd8defa38508ec9f2025-08-20T02:57:36ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412012-05-01991697510.14720/aas.2012.99.1.1452420916Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditionsHelena LESAR0Barbara HLEBEC1Nataša ČERANIČ2Damijana KASTELEC3Zlata LUTHAR4University of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaLunačkova 21, 8233 Mirna, SloveniaVrhpolje 5, 6240 Kozina, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaBletilla striata is a terrestrial sympodial orchid. Substrates used for outdoor growing, differing in the mixture of added components and nutrients, were chosen for acclimatization of the asymbiotic propagated plants. A total of 651 Bletilla striata orchids were planted in 3 commercial substrates: ″Tonsubstrat″ (Ton), ″Baltisches substrat″ (Baltski) and ″Royal-Garden″ (Royal). Prior to acclimatization, the plants were 2.5 cm on average, with at least 2 leaves and 2 - 3 cm long root or roots. Fewest plants (3.1%) died in Ton substrate, 3.2% in Baltski substrate and 5.0% in Royal substrate. There were no statistically significant differences among substrates (p = 0.558) in the percentage of plants that died. Substrates used in combination with the chosen phenophase and established conditions were suitable for acclimatization of Bletilla striata orchids, whereby 95-97% of plants successfully adapted from heterotrophic to autotrophic conditions in a very short period of two months. The basic conditions for success are that plants are large and vital enough prior to acclimatization, that the substrate is appropriate and that appropriate conditions of relative humidity, temperature, light and ventilation without major fluctuations of these factors are ensured during acclimatization.https://journals.uni-lj.si/aas/article/view/14524ornamental orchidbletilla striatatissue cultureacclimatizationsubstrategrowthdevelopment |
| spellingShingle | Helena LESAR Barbara HLEBEC Nataša ČERANIČ Damijana KASTELEC Zlata LUTHAR Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions Acta Agriculturae Slovenica ornamental orchid bletilla striata tissue culture acclimatization substrate growth development |
| title | Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions |
| title_full | Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions |
| title_fullStr | Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions |
| title_full_unstemmed | Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions |
| title_short | Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions |
| title_sort | acclimatization of terrestrial orchid bletilla striata rchb f orchidaceae propagated under in vitro conditions |
| topic | ornamental orchid bletilla striata tissue culture acclimatization substrate growth development |
| url | https://journals.uni-lj.si/aas/article/view/14524 |
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