Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury. Regulation of shifting microglia polarization from M1 (neurotoxic and proinflammatory type) to M2 (neuroprotective...

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Main Authors: Ziwei Fan, Mengxian Jia, Jian Zhou, Zhoule Zhu, Yumin Wu, Xiaowu Lin, Yiming Qian, Jiashu Lian, Xin Hua, Jianhong Dong, Zheyu Fang, Yuqing Liu, Sibing Chen, Xiumin Xue, Juanqing Yue, Minyu Zhu, Ying Wang, Zhihui Huang, Honglin Teng
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-11-01
Series:Neural Regeneration Research
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Online Access:https://journals.lww.com/10.4103/NRR.NRR-D-23-01256
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author Ziwei Fan
Mengxian Jia
Jian Zhou
Zhoule Zhu
Yumin Wu
Xiaowu Lin
Yiming Qian
Jiashu Lian
Xin Hua
Jianhong Dong
Zheyu Fang
Yuqing Liu
Sibing Chen
Xiumin Xue
Juanqing Yue
Minyu Zhu
Ying Wang
Zhihui Huang
Honglin Teng
author_facet Ziwei Fan
Mengxian Jia
Jian Zhou
Zhoule Zhu
Yumin Wu
Xiaowu Lin
Yiming Qian
Jiashu Lian
Xin Hua
Jianhong Dong
Zheyu Fang
Yuqing Liu
Sibing Chen
Xiumin Xue
Juanqing Yue
Minyu Zhu
Ying Wang
Zhihui Huang
Honglin Teng
author_sort Ziwei Fan
collection DOAJ
description The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury. Regulation of shifting microglia polarization from M1 (neurotoxic and proinflammatory type) to M2 (neuroprotective and anti-inflammatory type) after spinal cord injury appears to be crucial. Tryptanthrin possesses an anti-inflammatory biological function. However, its roles and the underlying molecular mechanisms in spinal cord injury remain unknown. In this study, we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in vitro. Tryptanthrin promoted M2 polarization through inactivating the cGAS/STING/NF-κB pathway. Additionally, we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury, inhibited neuronal loss, and promoted tissue repair and functional recovery in a mouse model of spinal cord injury. Finally, using a conditional co-culture system, we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress–related neuronal apoptosis. Taken together, these results suggest that by targeting the cGAS/STING/NF-κB axis, tryptanthrin attenuates microglia–derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.
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institution Kabale University
issn 1673-5374
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language English
publishDate 2025-11-01
publisher Wolters Kluwer Medknow Publications
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spelling doaj-art-73c5a9401ef14632b4faf96d975a21962025-01-07T09:49:28ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53741876-79582025-11-0120113287330110.4103/NRR.NRR-D-23-01256Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injuryZiwei FanMengxian JiaJian ZhouZhoule ZhuYumin WuXiaowu LinYiming QianJiashu LianXin HuaJianhong DongZheyu FangYuqing LiuSibing ChenXiumin XueJuanqing YueMinyu ZhuYing WangZhihui HuangHonglin TengThe M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury. Regulation of shifting microglia polarization from M1 (neurotoxic and proinflammatory type) to M2 (neuroprotective and anti-inflammatory type) after spinal cord injury appears to be crucial. Tryptanthrin possesses an anti-inflammatory biological function. However, its roles and the underlying molecular mechanisms in spinal cord injury remain unknown. In this study, we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in vitro. Tryptanthrin promoted M2 polarization through inactivating the cGAS/STING/NF-κB pathway. Additionally, we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury, inhibited neuronal loss, and promoted tissue repair and functional recovery in a mouse model of spinal cord injury. Finally, using a conditional co-culture system, we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress–related neuronal apoptosis. Taken together, these results suggest that by targeting the cGAS/STING/NF-κB axis, tryptanthrin attenuates microglia–derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.https://journals.lww.com/10.4103/NRR.NRR-D-23-01256cgas/stingfunctional recoverymicroglianeuroinflammationneuroprotectionnuclear factor-κbpolarizationspinal cord injurytryptanthrin
spellingShingle Ziwei Fan
Mengxian Jia
Jian Zhou
Zhoule Zhu
Yumin Wu
Xiaowu Lin
Yiming Qian
Jiashu Lian
Xin Hua
Jianhong Dong
Zheyu Fang
Yuqing Liu
Sibing Chen
Xiumin Xue
Juanqing Yue
Minyu Zhu
Ying Wang
Zhihui Huang
Honglin Teng
Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
Neural Regeneration Research
cgas/sting
functional recovery
microglia
neuroinflammation
neuroprotection
nuclear factor-κb
polarization
spinal cord injury
tryptanthrin
title Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
title_full Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
title_fullStr Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
title_full_unstemmed Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
title_short Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
title_sort pharmacological targeting cgas sting nf κb axis by tryptanthrin induces microglia polarization toward m2 phenotype and promotes functional recovery in a mouse model of spinal cord injury
topic cgas/sting
functional recovery
microglia
neuroinflammation
neuroprotection
nuclear factor-κb
polarization
spinal cord injury
tryptanthrin
url https://journals.lww.com/10.4103/NRR.NRR-D-23-01256
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