Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects
The purpose of our study was to determine whether the number of human very small embryonic-like stem cells (huVSELs) would vary depending on the age of humans. HuVSELs frequency was evaluated into the steady-state (SS) peripheral blood (PB) of healthy volunteers using flow cytometry analysis. Their...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7651645 |
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| author | Hanna Sovalat Maurice Scrofani Antoinette Eidenschenk Philippe Hénon |
| author_facet | Hanna Sovalat Maurice Scrofani Antoinette Eidenschenk Philippe Hénon |
| author_sort | Hanna Sovalat |
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| description | The purpose of our study was to determine whether the number of human very small embryonic-like stem cells (huVSELs) would vary depending on the age of humans. HuVSELs frequency was evaluated into the steady-state (SS) peripheral blood (PB) of healthy volunteers using flow cytometry analysis. Their numbers were compared with volunteers’ age. Blood samples were withdrawn from 28 volunteers (age ranging from 20 to 70 years), who were distributed among three groups of age: “young” (mean age, 27.8 years), “middle” (mean age, 49 years), and “older” (mean age, 64.2 years). Comparing the three groups, we did not observe any statistically significant difference in huVSELs numbers between them. The difference in mRNA expression for PSC markers as SSEA-4, Oct-4, Nanog, and Sox2 between the three groups of age was not statistically significant. A similar frequency of huVSELs into the SS-PB of young, middle-aged, and aged subjects may indicate that the VSELs pool persists all along the life as a reserve for tissue repair in case of minor injury and that there is a continuous efflux of these cells from the BM into the PB. |
| format | Article |
| id | doaj-art-73c53d5f2eff4327a636c48df5b8bbcc |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-73c53d5f2eff4327a636c48df5b8bbcc2025-08-20T02:07:05ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/76516457651645Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged SubjectsHanna Sovalat0Maurice Scrofani1Antoinette Eidenschenk2Philippe Hénon3Institut de Recherche en Hématologie et Transplantation, Hôpital du Hasenrain, 87 avenue d’Altkirch, 68100 Mulhouse, FranceInstitut de Recherche en Hématologie et Transplantation, Hôpital du Hasenrain, 87 avenue d’Altkirch, 68100 Mulhouse, FranceInstitut de Recherche en Hématologie et Transplantation, Hôpital du Hasenrain, 87 avenue d’Altkirch, 68100 Mulhouse, FranceInstitut de Recherche en Hématologie et Transplantation, Hôpital du Hasenrain, 87 avenue d’Altkirch, 68100 Mulhouse, FranceThe purpose of our study was to determine whether the number of human very small embryonic-like stem cells (huVSELs) would vary depending on the age of humans. HuVSELs frequency was evaluated into the steady-state (SS) peripheral blood (PB) of healthy volunteers using flow cytometry analysis. Their numbers were compared with volunteers’ age. Blood samples were withdrawn from 28 volunteers (age ranging from 20 to 70 years), who were distributed among three groups of age: “young” (mean age, 27.8 years), “middle” (mean age, 49 years), and “older” (mean age, 64.2 years). Comparing the three groups, we did not observe any statistically significant difference in huVSELs numbers between them. The difference in mRNA expression for PSC markers as SSEA-4, Oct-4, Nanog, and Sox2 between the three groups of age was not statistically significant. A similar frequency of huVSELs into the SS-PB of young, middle-aged, and aged subjects may indicate that the VSELs pool persists all along the life as a reserve for tissue repair in case of minor injury and that there is a continuous efflux of these cells from the BM into the PB.http://dx.doi.org/10.1155/2016/7651645 |
| spellingShingle | Hanna Sovalat Maurice Scrofani Antoinette Eidenschenk Philippe Hénon Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects Stem Cells International |
| title | Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects |
| title_full | Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects |
| title_fullStr | Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects |
| title_full_unstemmed | Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects |
| title_short | Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects |
| title_sort | human very small embryonic like stem cells are present in normal peripheral blood of young middle aged and aged subjects |
| url | http://dx.doi.org/10.1155/2016/7651645 |
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