Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers
Background. DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected Fragaria species from northern Pakistan using DNA barcode markers. Methodology. Initially,...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/9249561 |
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| author | Awais Qarni Khushi Muhammad Abdul Wahab Amir Ali Chandni Khizar Izhar Ullah Abeer Kazmi Tahira Sultana Asma Hameed Muhammad Younas Mehdi Rahimi |
| author_facet | Awais Qarni Khushi Muhammad Abdul Wahab Amir Ali Chandni Khizar Izhar Ullah Abeer Kazmi Tahira Sultana Asma Hameed Muhammad Younas Mehdi Rahimi |
| author_sort | Awais Qarni |
| collection | DOAJ |
| description | Background. DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected Fragaria species from northern Pakistan using DNA barcode markers. Methodology. Initially, the efficacy of eight DNA barcode markers was analyzed based on the amplification and sequencing of the genome of selected Fragaria species. The resultant sequences were analyzed using BLAST, MEGA 7.0, and Bio Edit software. The phylogenetic tree was constructed by using Fragaria current species sequences and reference sequences through the neighbor-joining method or maximum likelihood method. Results. Among eight DNA barcode markers, only two (ITS2 and rbclC) were amplified, and sequences were obtained. ITS2 sequence was BLAST in NCBI for related reference species which ranged from 89.79% to 90.05% along with Fragaria vesca (AF163517.1) which have 99.05% identity. Similarly, the rbclC sequence of Fragaria species was ranged from 96% to 99.58% along with Fragaria × ananassa (KY358226.1) which had 99.58% identity. Conclusion. It is recommended that DNA barcode markers are a useful tool to identify the genetic diversity of a species. Moreover, this study could be helpful for the identification of the Fragaria species cultivated in other regions of the world. |
| format | Article |
| id | doaj-art-73c00198c76b4f8d93a0f122fa7141bd |
| institution | Kabale University |
| issn | 1469-5073 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-73c00198c76b4f8d93a0f122fa7141bd2025-02-03T06:11:52ZengWileyGenetics Research1469-50732022-01-01202210.1155/2022/9249561Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode MarkersAwais Qarni0Khushi Muhammad1Abdul Wahab2Amir Ali3Chandni Khizar4Izhar Ullah5Abeer Kazmi6Tahira Sultana7Asma Hameed8Muhammad Younas9Mehdi Rahimi10Department of GeneticsDepartment of GeneticsShanghai Center for Plant Stress BiologyDepartment of BotanyInstitute of Molecular Biology and BiotechnologyDepartment of BotanyInstitute of HydrobiologyDepartment of BotanyDepartment of BotanyDepartment of BotanyDepartment of BiotechnologyBackground. DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected Fragaria species from northern Pakistan using DNA barcode markers. Methodology. Initially, the efficacy of eight DNA barcode markers was analyzed based on the amplification and sequencing of the genome of selected Fragaria species. The resultant sequences were analyzed using BLAST, MEGA 7.0, and Bio Edit software. The phylogenetic tree was constructed by using Fragaria current species sequences and reference sequences through the neighbor-joining method or maximum likelihood method. Results. Among eight DNA barcode markers, only two (ITS2 and rbclC) were amplified, and sequences were obtained. ITS2 sequence was BLAST in NCBI for related reference species which ranged from 89.79% to 90.05% along with Fragaria vesca (AF163517.1) which have 99.05% identity. Similarly, the rbclC sequence of Fragaria species was ranged from 96% to 99.58% along with Fragaria × ananassa (KY358226.1) which had 99.58% identity. Conclusion. It is recommended that DNA barcode markers are a useful tool to identify the genetic diversity of a species. Moreover, this study could be helpful for the identification of the Fragaria species cultivated in other regions of the world.http://dx.doi.org/10.1155/2022/9249561 |
| spellingShingle | Awais Qarni Khushi Muhammad Abdul Wahab Amir Ali Chandni Khizar Izhar Ullah Abeer Kazmi Tahira Sultana Asma Hameed Muhammad Younas Mehdi Rahimi Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers Genetics Research |
| title | Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers |
| title_full | Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers |
| title_fullStr | Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers |
| title_full_unstemmed | Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers |
| title_short | Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers |
| title_sort | molecular characterization of wild and cultivated strawberry fragaria ananassa through dna barcode markers |
| url | http://dx.doi.org/10.1155/2022/9249561 |
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