A Bis(Acridino)-Crown Ether for Recognizing Oligoamines in Spermine Biosynthesis
Oligoamines in cellular metabolism carry extremely diverse biological functions (i.e., regulating Ca<sup>2+</sup>-influx, neuronal nitric oxide synthase, membrane potential, Na<sup>+</sup>, K<sup>+</sup>-ATPase activity in synaptosomes, etc.). Furthermore, they al...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/18/4390 |
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| Summary: | Oligoamines in cellular metabolism carry extremely diverse biological functions (i.e., regulating Ca<sup>2+</sup>-influx, neuronal nitric oxide synthase, membrane potential, Na<sup>+</sup>, K<sup>+</sup>-ATPase activity in synaptosomes, etc.). Furthermore, they also act as longevity agents and have a determinative role in autophagy, cell growth, proliferation, and death, while oligoamines dysregulation is a key in a variety of cancers. However, many of their mechanisms of actions have just begun to be understood. In addition to the numerous biosensing methods, only a very few simple small molecule-based tests are available for their selective but reversible tracking or fluorescent labeling. Motivated by this, we present herein a new fluorescent bis(acridino)-crown ether as a sensor molecule for biogenic oligoamines. The sensor molecule can selectively distinguish oligoamines from aliphatic mono- and diamino-analogues, while showing a reversible 1:2 (host:guest) complexation with a stepwise binding process accompanied by a turn-on fluorescence response. Both computational simulations on molecular docking and regression methods on titration experiments were carried out to reveal the oligoamine-recognition properties of the sensor molecule. The new fluorescent chemosensor molecule has a high potential for molecular-level functional studies on the oligoamine systems in cell processes (cellular uptake, transport, progression in cancers, etc.). |
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| ISSN: | 1420-3049 |