Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms
Thoracic aortic aneurysm develops as a result of complex series of events that alter the cellular structure and the composition of the extracellular matrix of the aortic wall. The purpose of the present work was to study the cellular functions of endothelial and smooth muscle cells from the patients...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2016/3107879 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547644420915200 |
|---|---|
| author | Anna Malashicheva Daria Kostina Aleksandra Kostina Olga Irtyuga Irina Voronkina Larisa Smagina Elena Ignatieva Natalia Gavriliuk Vladimir Uspensky Olga Moiseeva Jarle Vaage Anna Kostareva |
| author_facet | Anna Malashicheva Daria Kostina Aleksandra Kostina Olga Irtyuga Irina Voronkina Larisa Smagina Elena Ignatieva Natalia Gavriliuk Vladimir Uspensky Olga Moiseeva Jarle Vaage Anna Kostareva |
| author_sort | Anna Malashicheva |
| collection | DOAJ |
| description | Thoracic aortic aneurysm develops as a result of complex series of events that alter the cellular structure and the composition of the extracellular matrix of the aortic wall. The purpose of the present work was to study the cellular functions of endothelial and smooth muscle cells from the patients with aneurysms of the thoracic aorta. We studied endothelial and smooth muscle cells from aneurysms in patients with bicuspid aortic valve and with tricuspid aortic valve. The expression of key markers of endothelial (CD31, vWF, and VE-cadherin) and smooth muscle (SMA, SM22α, calponin, and vimentin) cells as well extracellular matrix and MMP activity was studied as well as and apoptosis and cell proliferation. Expression of functional markers of endothelial and smooth muscle cells was reduced in patient cells. Cellular proliferation, migration, and synthesis of extracellular matrix proteins are attenuated in the cells of the patients. We show for the first time that aortic endothelial cell phenotype is changed in the thoracic aortic aneurysms compared to normal aortic wall. In conclusion both endothelial and smooth muscle cells from aneurysms of the ascending aorta have downregulated specific cellular markers and altered functional properties, such as growth rate, apoptosis induction, and extracellular matrix synthesis. |
| format | Article |
| id | doaj-art-73ba1b26bb3c4f3cb9c7e8a2b578d08d |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-73ba1b26bb3c4f3cb9c7e8a2b578d08d2025-02-03T06:43:54ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322016-01-01201610.1155/2016/31078793107879Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic AneurysmsAnna Malashicheva0Daria Kostina1Aleksandra Kostina2Olga Irtyuga3Irina Voronkina4Larisa Smagina5Elena Ignatieva6Natalia Gavriliuk7Vladimir Uspensky8Olga Moiseeva9Jarle Vaage10Anna Kostareva11Almazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Avenue 4, Saint Petersburg 194064, RussiaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Avenue 4, Saint Petersburg 194064, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaInstitute of Clinical Medicine, University of Oslo and Oslo University Hospital, Postboks 1171, Blindern, 0318 Oslo, NorwayAlmazov Federal Medical Research Centre, Akkuratova 2, Saint Petersburg 197341, RussiaThoracic aortic aneurysm develops as a result of complex series of events that alter the cellular structure and the composition of the extracellular matrix of the aortic wall. The purpose of the present work was to study the cellular functions of endothelial and smooth muscle cells from the patients with aneurysms of the thoracic aorta. We studied endothelial and smooth muscle cells from aneurysms in patients with bicuspid aortic valve and with tricuspid aortic valve. The expression of key markers of endothelial (CD31, vWF, and VE-cadherin) and smooth muscle (SMA, SM22α, calponin, and vimentin) cells as well extracellular matrix and MMP activity was studied as well as and apoptosis and cell proliferation. Expression of functional markers of endothelial and smooth muscle cells was reduced in patient cells. Cellular proliferation, migration, and synthesis of extracellular matrix proteins are attenuated in the cells of the patients. We show for the first time that aortic endothelial cell phenotype is changed in the thoracic aortic aneurysms compared to normal aortic wall. In conclusion both endothelial and smooth muscle cells from aneurysms of the ascending aorta have downregulated specific cellular markers and altered functional properties, such as growth rate, apoptosis induction, and extracellular matrix synthesis.http://dx.doi.org/10.1155/2016/3107879 |
| spellingShingle | Anna Malashicheva Daria Kostina Aleksandra Kostina Olga Irtyuga Irina Voronkina Larisa Smagina Elena Ignatieva Natalia Gavriliuk Vladimir Uspensky Olga Moiseeva Jarle Vaage Anna Kostareva Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms International Journal of Vascular Medicine |
| title | Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms |
| title_full | Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms |
| title_fullStr | Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms |
| title_full_unstemmed | Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms |
| title_short | Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms |
| title_sort | phenotypic and functional changes of endothelial and smooth muscle cells in thoracic aortic aneurysms |
| url | http://dx.doi.org/10.1155/2016/3107879 |
| work_keys_str_mv | AT annamalashicheva phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT dariakostina phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT aleksandrakostina phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT olgairtyuga phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT irinavoronkina phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT larisasmagina phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT elenaignatieva phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT nataliagavriliuk phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT vladimiruspensky phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT olgamoiseeva phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT jarlevaage phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms AT annakostareva phenotypicandfunctionalchangesofendothelialandsmoothmusclecellsinthoracicaorticaneurysms |