The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation.
The RNA chaperone Hfq regulates virulence and metabolism in the opportunistic pathogen Pseudomonas aeruginosa. During carbon catabolite repression (CCR) Hfq together with the catabolite repression control protein Crc can act as a translational repressor of catabolic genes. Upon relief of CCR, the le...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180887&type=printable |
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| author | Elisabeth Sonnleitner Konstantin Prindl Udo Bläsi |
| author_facet | Elisabeth Sonnleitner Konstantin Prindl Udo Bläsi |
| author_sort | Elisabeth Sonnleitner |
| collection | DOAJ |
| description | The RNA chaperone Hfq regulates virulence and metabolism in the opportunistic pathogen Pseudomonas aeruginosa. During carbon catabolite repression (CCR) Hfq together with the catabolite repression control protein Crc can act as a translational repressor of catabolic genes. Upon relief of CCR, the level of the Hfq-titrating RNA CrcZ is increasing, which in turn abrogates Hfq-mediated translational repression. As the interdependence of Hfq-mediated and RNA based control mechanisms is poorly understood, we explored the possibility whether the regulatory RNA CrcZ can interfere with riboregulation. We first substantiate that the P. aeruginosa Hfq is proficient and required for riboregulation of the transcriptional activator gene antR by the small RNA PrrF1-2. Our studies further revealed that CrcZ can interfere with PrrF1-2/Hfq-mediated regulation of antR. The competition for Hfq can be rationalized by the higher affinity of Hfq for CrcZ than for antR mRNA. |
| format | Article |
| id | doaj-art-73a896c60fe243e5b834d1dc58de4cb2 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-73a896c60fe243e5b834d1dc58de4cb22025-08-20T02:31:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018088710.1371/journal.pone.0180887The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation.Elisabeth SonnleitnerKonstantin PrindlUdo BläsiThe RNA chaperone Hfq regulates virulence and metabolism in the opportunistic pathogen Pseudomonas aeruginosa. During carbon catabolite repression (CCR) Hfq together with the catabolite repression control protein Crc can act as a translational repressor of catabolic genes. Upon relief of CCR, the level of the Hfq-titrating RNA CrcZ is increasing, which in turn abrogates Hfq-mediated translational repression. As the interdependence of Hfq-mediated and RNA based control mechanisms is poorly understood, we explored the possibility whether the regulatory RNA CrcZ can interfere with riboregulation. We first substantiate that the P. aeruginosa Hfq is proficient and required for riboregulation of the transcriptional activator gene antR by the small RNA PrrF1-2. Our studies further revealed that CrcZ can interfere with PrrF1-2/Hfq-mediated regulation of antR. The competition for Hfq can be rationalized by the higher affinity of Hfq for CrcZ than for antR mRNA.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180887&type=printable |
| spellingShingle | Elisabeth Sonnleitner Konstantin Prindl Udo Bläsi The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. PLoS ONE |
| title | The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. |
| title_full | The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. |
| title_fullStr | The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. |
| title_full_unstemmed | The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. |
| title_short | The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation. |
| title_sort | pseudomonas aeruginosa crcz rna interferes with hfq mediated riboregulation |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180887&type=printable |
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