Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber
The host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>),...
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2025-02-01
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| author | Ernest Nailevich Komissarov Roderic Gilles Claret Diabankana Inna Abdeeva Daniel Mawuena Afordoanyi Sergey Vladimirovich Gudkov Ekaterina Mikhailovna Dvorianinova Sergey Alexandrovich Bruskin Alexey Alexandrovich Dmitriev Shamil Zavdatovich Validov |
| author_facet | Ernest Nailevich Komissarov Roderic Gilles Claret Diabankana Inna Abdeeva Daniel Mawuena Afordoanyi Sergey Vladimirovich Gudkov Ekaterina Mikhailovna Dvorianinova Sergey Alexandrovich Bruskin Alexey Alexandrovich Dmitriev Shamil Zavdatovich Validov |
| author_sort | Ernest Nailevich Komissarov |
| collection | DOAJ |
| description | The host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>), normally distributed on the accessory chromosomes. The pattern of <i>mimp</i> associated with effector genes has been used to predict candidate effector profiles which characterize <i>Fox</i> formae speciales. In this study, we demonstrate the pathogenicity of strains <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407 and <i>Fusarium oxysporum</i> f.sp. <i>radicis-cucumerinum</i> (<i>Forc</i>) V03-2g in a common host plant (cucumber) and compare their genomes. The <i>Forl</i> ZUM2407 genome lacks <i>SIX</i> genes and their homologs, in contrast to <i>Forc</i> V03-2g. We predicted the total number of <i>mimp</i> elements in the genome of <i>Forl</i> ZUM2407 to be three-fold less than that of <i>Forc</i> V03-2g (10 and 36 copies, respectively). The <i>mimp</i> distribution pattern in <i>Forl</i> ZUM2407 was completely different from that present in <i>Forc</i> V03-2g. Candidate effector profile analysis did not predict that <i>Forl</i> ZUM2407 was able to infect cucumber plants like <i>Forc</i> V03-2g. Therefore, we assume that <i>Forl</i> ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the <i>mimp</i>-based approach. |
| format | Article |
| id | doaj-art-73a64dfe937744f088b02b67d82ae05b |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-02-01 |
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| series | Journal of Fungi |
| spelling | doaj-art-73a64dfe937744f088b02b67d82ae05b2025-08-20T02:45:00ZengMDPI AGJournal of Fungi2309-608X2025-02-0111214010.3390/jof11020140Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in CucumberErnest Nailevich Komissarov0Roderic Gilles Claret Diabankana1Inna Abdeeva2Daniel Mawuena Afordoanyi3Sergey Vladimirovich Gudkov4Ekaterina Mikhailovna Dvorianinova5Sergey Alexandrovich Bruskin6Alexey Alexandrovich Dmitriev7Shamil Zavdatovich Validov8Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaVavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaProkhorov General Physics Institute of Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaVavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of the Russian Academy of Sciences, 420111 Kazan, RussiaThe host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>), normally distributed on the accessory chromosomes. The pattern of <i>mimp</i> associated with effector genes has been used to predict candidate effector profiles which characterize <i>Fox</i> formae speciales. In this study, we demonstrate the pathogenicity of strains <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407 and <i>Fusarium oxysporum</i> f.sp. <i>radicis-cucumerinum</i> (<i>Forc</i>) V03-2g in a common host plant (cucumber) and compare their genomes. The <i>Forl</i> ZUM2407 genome lacks <i>SIX</i> genes and their homologs, in contrast to <i>Forc</i> V03-2g. We predicted the total number of <i>mimp</i> elements in the genome of <i>Forl</i> ZUM2407 to be three-fold less than that of <i>Forc</i> V03-2g (10 and 36 copies, respectively). The <i>mimp</i> distribution pattern in <i>Forl</i> ZUM2407 was completely different from that present in <i>Forc</i> V03-2g. Candidate effector profile analysis did not predict that <i>Forl</i> ZUM2407 was able to infect cucumber plants like <i>Forc</i> V03-2g. Therefore, we assume that <i>Forl</i> ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the <i>mimp</i>-based approach.https://www.mdpi.com/2309-608X/11/2/140<i>Fusarium oxysporum</i><i>radicis-lycopersici</i><i>radicis-cucumerinum</i>root rot<i>SIX</i> genes<i>mimp</i> |
| spellingShingle | Ernest Nailevich Komissarov Roderic Gilles Claret Diabankana Inna Abdeeva Daniel Mawuena Afordoanyi Sergey Vladimirovich Gudkov Ekaterina Mikhailovna Dvorianinova Sergey Alexandrovich Bruskin Alexey Alexandrovich Dmitriev Shamil Zavdatovich Validov Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber Journal of Fungi <i>Fusarium oxysporum</i> <i>radicis-lycopersici</i> <i>radicis-cucumerinum</i> root rot <i>SIX</i> genes <i>mimp</i> |
| title | Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber |
| title_full | Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber |
| title_fullStr | Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber |
| title_full_unstemmed | Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber |
| title_short | Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber |
| title_sort | genomic differences between two i fusarium oxysporum i formae speciales causing root rot in cucumber |
| topic | <i>Fusarium oxysporum</i> <i>radicis-lycopersici</i> <i>radicis-cucumerinum</i> root rot <i>SIX</i> genes <i>mimp</i> |
| url | https://www.mdpi.com/2309-608X/11/2/140 |
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