Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells
Objective. To evaluate the relationship between TGFβ signaling and endothelial lncRNA expression. Methods. Human umbilical vein endothelial cell (HUVECs) lncRNAs and mRNAs were profiled with the Arraystar Human lncRNA Expression Microarray V3.0 after 24 hours of exposure to TGFβ1 (10 ng/mL). Results...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2016/2459687 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560739058974720 |
|---|---|
| author | Krishna K. Singh Pratiek N. Matkar Adrian Quan Laura-Eve Mantella Hwee Teoh Mohammed Al-Omran Subodh Verma |
| author_facet | Krishna K. Singh Pratiek N. Matkar Adrian Quan Laura-Eve Mantella Hwee Teoh Mohammed Al-Omran Subodh Verma |
| author_sort | Krishna K. Singh |
| collection | DOAJ |
| description | Objective. To evaluate the relationship between TGFβ signaling and endothelial lncRNA expression. Methods. Human umbilical vein endothelial cell (HUVECs) lncRNAs and mRNAs were profiled with the Arraystar Human lncRNA Expression Microarray V3.0 after 24 hours of exposure to TGFβ1 (10 ng/mL). Results. Of the 30,584 lncRNAs screened, 2,051 were significantly upregulated and 2,393 were appreciably downregulated (P<0.05) in response to TGFβ. In the same HUVEC samples, 2,148 of the 26,106 mRNAs screened were upregulated and 1,290 were downregulated. Of these 2,051 differentially expressed upregulated lncRNAs, MALAT1, which is known to be induced by TGFβ in endothelial cells, was the most (~220-fold) upregulated lncRNA. Bioinformatics analyses indicated that the differentially expressed upregulated mRNAs are primarily enriched in hippo signaling, Wnt signaling, focal adhesion, neuroactive ligand-receptor interaction, and pathways in cancer. The most downregulated are notably involved in olfactory transduction, PI3-Akt signaling, Ras signaling, neuroactive ligand-receptor interaction, and apoptosis. Conclusions. This is the first lncRNA and mRNA transcriptome profile of TGFβ-mediated changes in human endothelial cells. These observations may reveal potential new targets of TGFβ in endothelial cells and novel therapeutic avenues for cardiovascular disease-associated endothelial dysfunction. |
| format | Article |
| id | doaj-art-7396a5f1592b451f8a6b7ed439eb61bf |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-7396a5f1592b451f8a6b7ed439eb61bf2025-02-03T01:26:51ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322016-01-01201610.1155/2016/24596872459687Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial CellsKrishna K. Singh0Pratiek N. Matkar1Adrian Quan2Laura-Eve Mantella3Hwee Teoh4Mohammed Al-Omran5Subodh Verma6Division of Vascular Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaInstitute of Medical Science, University of Toronto, Toronto, ON, M5S 1A8, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaDivision of Vascular Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Toronto, ON, M5B 1W8, CanadaObjective. To evaluate the relationship between TGFβ signaling and endothelial lncRNA expression. Methods. Human umbilical vein endothelial cell (HUVECs) lncRNAs and mRNAs were profiled with the Arraystar Human lncRNA Expression Microarray V3.0 after 24 hours of exposure to TGFβ1 (10 ng/mL). Results. Of the 30,584 lncRNAs screened, 2,051 were significantly upregulated and 2,393 were appreciably downregulated (P<0.05) in response to TGFβ. In the same HUVEC samples, 2,148 of the 26,106 mRNAs screened were upregulated and 1,290 were downregulated. Of these 2,051 differentially expressed upregulated lncRNAs, MALAT1, which is known to be induced by TGFβ in endothelial cells, was the most (~220-fold) upregulated lncRNA. Bioinformatics analyses indicated that the differentially expressed upregulated mRNAs are primarily enriched in hippo signaling, Wnt signaling, focal adhesion, neuroactive ligand-receptor interaction, and pathways in cancer. The most downregulated are notably involved in olfactory transduction, PI3-Akt signaling, Ras signaling, neuroactive ligand-receptor interaction, and apoptosis. Conclusions. This is the first lncRNA and mRNA transcriptome profile of TGFβ-mediated changes in human endothelial cells. These observations may reveal potential new targets of TGFβ in endothelial cells and novel therapeutic avenues for cardiovascular disease-associated endothelial dysfunction.http://dx.doi.org/10.1155/2016/2459687 |
| spellingShingle | Krishna K. Singh Pratiek N. Matkar Adrian Quan Laura-Eve Mantella Hwee Teoh Mohammed Al-Omran Subodh Verma Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells International Journal of Vascular Medicine |
| title | Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells |
| title_full | Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells |
| title_fullStr | Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells |
| title_full_unstemmed | Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells |
| title_short | Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells |
| title_sort | investigation of tgfβ1 induced long noncoding rnas in endothelial cells |
| url | http://dx.doi.org/10.1155/2016/2459687 |
| work_keys_str_mv | AT krishnaksingh investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT pratieknmatkar investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT adrianquan investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT lauraevemantella investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT hweeteoh investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT mohammedalomran investigationoftgfb1inducedlongnoncodingrnasinendothelialcells AT subodhverma investigationoftgfb1inducedlongnoncodingrnasinendothelialcells |