A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow
By utilizing polydimethylsiloxane (PDMS), collagen hydrogel, and a cell line for human cerebral microvascular endothelial cells, we produced a 3D microchannel blood–brain barrier (BBB) model under physiological flow. This 3D BBB has a circular-shaped cross-section and a diameter of ~100 μm, which ca...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/14/6/456 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850203980793118720 |
|---|---|
| author | Yunfei Li Bingmei M. Fu |
| author_facet | Yunfei Li Bingmei M. Fu |
| author_sort | Yunfei Li |
| collection | DOAJ |
| description | By utilizing polydimethylsiloxane (PDMS), collagen hydrogel, and a cell line for human cerebral microvascular endothelial cells, we produced a 3D microchannel blood–brain barrier (BBB) model under physiological flow. This 3D BBB has a circular-shaped cross-section and a diameter of ~100 μm, which can properly mimic the cerebral microvessel responsible for material exchange between the circulating blood and brain tissue. The permeability of the 3D microchannel BBB to a small molecule (sodium fluorescein with a molecular weight of 376) and that to a large molecule (Dex-70k) are the same as those of rat cerebral microvessels. This 3D BBB model can replicate the effects of a plasma protein, orosomucoid, a cytokine, vascular endothelial growth factor (VEGF), and an enzyme, heparinase III, on either rat cerebral or mesenteric microvessesels in terms of permeability and the modulation of glycocalyx (heparan sulfate). It can also replicate the adhesion of a breast cancer cell, MDA-MB-231, in rat mesenteric microvessels under no treatment or treatments with VEGF, orosomucoid, and heparinase III. Because of difficulties in accessing human cerebral microvessels, this inexpensive and easy to assemble 3D human BBB model can be applied to investigate BBB-modulating mechanisms in health and in disease and to develop therapeutic interventions targeting tumor metastasis to the brain. |
| format | Article |
| id | doaj-art-738bddd7bfed4b4c8c4d4b3cd5b625f1 |
| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-738bddd7bfed4b4c8c4d4b3cd5b625f12025-08-20T02:11:22ZengMDPI AGCells2073-44092025-03-0114645610.3390/cells14060456A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under FlowYunfei Li0Bingmei M. Fu1Department of Biomedical Engineering, The City College of the City University of New York, New York, NY 10031, USADepartment of Biomedical Engineering, The City College of the City University of New York, New York, NY 10031, USABy utilizing polydimethylsiloxane (PDMS), collagen hydrogel, and a cell line for human cerebral microvascular endothelial cells, we produced a 3D microchannel blood–brain barrier (BBB) model under physiological flow. This 3D BBB has a circular-shaped cross-section and a diameter of ~100 μm, which can properly mimic the cerebral microvessel responsible for material exchange between the circulating blood and brain tissue. The permeability of the 3D microchannel BBB to a small molecule (sodium fluorescein with a molecular weight of 376) and that to a large molecule (Dex-70k) are the same as those of rat cerebral microvessels. This 3D BBB model can replicate the effects of a plasma protein, orosomucoid, a cytokine, vascular endothelial growth factor (VEGF), and an enzyme, heparinase III, on either rat cerebral or mesenteric microvessesels in terms of permeability and the modulation of glycocalyx (heparan sulfate). It can also replicate the adhesion of a breast cancer cell, MDA-MB-231, in rat mesenteric microvessels under no treatment or treatments with VEGF, orosomucoid, and heparinase III. Because of difficulties in accessing human cerebral microvessels, this inexpensive and easy to assemble 3D human BBB model can be applied to investigate BBB-modulating mechanisms in health and in disease and to develop therapeutic interventions targeting tumor metastasis to the brain.https://www.mdpi.com/2073-4409/14/6/456engineered microvesselmicrofluidic devicesolute permeabilitysodium fluoresceindextran-70kglycocalyx |
| spellingShingle | Yunfei Li Bingmei M. Fu A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow Cells engineered microvessel microfluidic device solute permeability sodium fluorescein dextran-70k glycocalyx |
| title | A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow |
| title_full | A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow |
| title_fullStr | A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow |
| title_full_unstemmed | A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow |
| title_short | A Cost-Effective and Easy to Assemble 3D Human Microchannel Blood–Brain Barrier Model and Its Application in Tumor Cell Adhesion Under Flow |
| title_sort | cost effective and easy to assemble 3d human microchannel blood brain barrier model and its application in tumor cell adhesion under flow |
| topic | engineered microvessel microfluidic device solute permeability sodium fluorescein dextran-70k glycocalyx |
| url | https://www.mdpi.com/2073-4409/14/6/456 |
| work_keys_str_mv | AT yunfeili acosteffectiveandeasytoassemble3dhumanmicrochannelbloodbrainbarriermodelanditsapplicationintumorcelladhesionunderflow AT bingmeimfu acosteffectiveandeasytoassemble3dhumanmicrochannelbloodbrainbarriermodelanditsapplicationintumorcelladhesionunderflow AT yunfeili costeffectiveandeasytoassemble3dhumanmicrochannelbloodbrainbarriermodelanditsapplicationintumorcelladhesionunderflow AT bingmeimfu costeffectiveandeasytoassemble3dhumanmicrochannelbloodbrainbarriermodelanditsapplicationintumorcelladhesionunderflow |