Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway
Abstract Bone regeneration is a critical area of research in the treatment of bone defects within clinical practice. Molybdenum (Mo) is an essential trace element for many organisms, which plays a vital role in various cellular activities, such as maintaining energy metabolism and promoting cell pro...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-07298-7 |
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| author | Jing Xia Jue Yu Wenwen Shi Yan Liu |
| author_facet | Jing Xia Jue Yu Wenwen Shi Yan Liu |
| author_sort | Jing Xia |
| collection | DOAJ |
| description | Abstract Bone regeneration is a critical area of research in the treatment of bone defects within clinical practice. Molybdenum (Mo) is an essential trace element for many organisms, which plays a vital role in various cellular activities, such as maintaining energy metabolism and promoting cell proliferation. However, whether Mo regulates the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and promotes bone tissue regeneration remains unknown. This study confirmed the nourishing effect of Mo on the osteogenic differentiation of PDLSCs, as evidenced by higher expression of osteogenic-related markers (OCN, BSP, OSX and RUNX2), ALP activity and mineralization ability. Moreover, through constructing compound material GelMA + PDLSC, we proved that Mo promoted PDLSC-based bone regeneration quantity and quality in rat calvarial defects. Finally, we revealed the underlying mechanism that the JAK/STAT3 signaling pathway actively participated in the Mo-enhanced osteogenic differentiation of PDLSCs. The inhibition of STAT3 diminished the benefit of Mo. Overall, our study found that Mo facilitated PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway, which may shed new light on clinical bone regeneration therapeutic strategies. |
| format | Article |
| id | doaj-art-7382b98e1a854536b080809127f1f496 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7382b98e1a854536b080809127f1f4962025-08-20T03:37:27ZengNature PortfolioScientific Reports2045-23222025-07-011511810.1038/s41598-025-07298-7Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathwayJing Xia0Jue Yu1Wenwen Shi2Yan Liu3Department of Clinical Microbiology, Shanghai Tenth People’s Hospital, School of Medicine, Tongji UniversityDepartment of Clinical Laboratory, Shanghai Tenth People’s Hospital, School of Medicine, Tongji UniversityDepartment of Clinical Immunology, Shanghai Tenth People’s Hospital, School of Medicine, Tongji UniversityDepartment of Clinical Microbiology, Shanghai Tenth People’s Hospital, School of Medicine, Tongji UniversityAbstract Bone regeneration is a critical area of research in the treatment of bone defects within clinical practice. Molybdenum (Mo) is an essential trace element for many organisms, which plays a vital role in various cellular activities, such as maintaining energy metabolism and promoting cell proliferation. However, whether Mo regulates the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and promotes bone tissue regeneration remains unknown. This study confirmed the nourishing effect of Mo on the osteogenic differentiation of PDLSCs, as evidenced by higher expression of osteogenic-related markers (OCN, BSP, OSX and RUNX2), ALP activity and mineralization ability. Moreover, through constructing compound material GelMA + PDLSC, we proved that Mo promoted PDLSC-based bone regeneration quantity and quality in rat calvarial defects. Finally, we revealed the underlying mechanism that the JAK/STAT3 signaling pathway actively participated in the Mo-enhanced osteogenic differentiation of PDLSCs. The inhibition of STAT3 diminished the benefit of Mo. Overall, our study found that Mo facilitated PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway, which may shed new light on clinical bone regeneration therapeutic strategies.https://doi.org/10.1038/s41598-025-07298-7Bone regenerationPeriodontal ligament stem cellMolybdenumOsteogenic differentiation |
| spellingShingle | Jing Xia Jue Yu Wenwen Shi Yan Liu Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway Scientific Reports Bone regeneration Periodontal ligament stem cell Molybdenum Osteogenic differentiation |
| title | Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway |
| title_full | Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway |
| title_fullStr | Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway |
| title_full_unstemmed | Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway |
| title_short | Molybdenum facilitates PDLSC-based bone regeneration through the JAK/STAT3 signaling pathway |
| title_sort | molybdenum facilitates pdlsc based bone regeneration through the jak stat3 signaling pathway |
| topic | Bone regeneration Periodontal ligament stem cell Molybdenum Osteogenic differentiation |
| url | https://doi.org/10.1038/s41598-025-07298-7 |
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