A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis
Introduction: Neonatal sepsis is a clinical syndrome described as any systemic bacterial infection in neonates documented by positive blood culture. However, blood culture is positive in only 5.0%–10.0% of suspected cases. Serum concentration of many acute-phase reactants rises in response to infect...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-04-01
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| Series: | Pediatric Respirology and Critical Care Medicine |
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| Online Access: | https://journals.lww.com/10.4103/prcm.prcm_11_22 |
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| author | Fauzia Talat Kiran Alam Kafil Akhtar S. Monazir Ali |
| author_facet | Fauzia Talat Kiran Alam Kafil Akhtar S. Monazir Ali |
| author_sort | Fauzia Talat |
| collection | DOAJ |
| description | Introduction:
Neonatal sepsis is a clinical syndrome described as any systemic bacterial infection in neonates documented by positive blood culture. However, blood culture is positive in only 5.0%–10.0% of suspected cases. Serum concentration of many acute-phase reactants rises in response to infection, which can be used as a non-specific indicator of bacterial sepsis.
Aim and Objectives:
The aim of this study was to correlate the levels of serum markers C-reactive protein (CRP), serum ferritin, and thrombocytopenia with neonatal sepsis.
Materials and Methods:
This was a prospective cross-sectional study conducted in the Neonatal Intensive Care Unit, Department of Paediatrics and Pathology, Jawaharlal Nehru Medical College (JNMC), Aligarh from 2019 to 2021 on 172 babies (cases =142; controls = 30). Neonates with sepsis who presented with clinical signs or symptoms of sepsis were taken as case group and healthy neonates served as control.
Result:
Blood culture was positive in 58 (40.8%) neonates in the case group and Klebsiella was present in maximum number of cases. Blood culture was positive in only 8 (13.8%) cases out of 31 cases of mild thrombocytopenia. The total culture-positive organism was 58 (40.8%), with 09 (15.5%) gram-positive, 46 (79.3%) gram-negative organisms, and 03(5.2%) fungus. Positive CRP was seen in 88 (61.9%) neonates in the case group, out of which, positive culture was noted in 38 (65.5%) neonates and negative in 50 (59.5%) neonates. Serum ferritin values >400 µgm/L was seen in 97 (68.3%) neonates in the case group and 6 (20.0%) neonates in control group. The mean serum ferritin in culture positive neonates was 1024 ± 309 µgm/L and in culture-negative neonates was 999 ± 301 µgm/L.
Conclusions:
The signs and symptoms of neonatal sepsis are non-specific, leading to difficulty in diagnosis and treatment. Biomarkers such as hematological indices, blood culture, and acute-phase reactants could be more reliable in rapid evaluation and early diagnosis of sepsis and may provide a new diagnostic strategy for the neonates with sepsis. |
| format | Article |
| id | doaj-art-7380a0e3b7ec44fb8b50ae97d96750ee |
| institution | OA Journals |
| issn | 2543-0343 2543-0351 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Pediatric Respirology and Critical Care Medicine |
| spelling | doaj-art-7380a0e3b7ec44fb8b50ae97d96750ee2025-08-20T02:18:58ZengWolters Kluwer Medknow PublicationsPediatric Respirology and Critical Care Medicine2543-03432543-03512022-04-0162273010.4103/prcm.prcm_11_22A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal SepsisFauzia TalatKiran AlamKafil AkhtarS. Monazir AliIntroduction: Neonatal sepsis is a clinical syndrome described as any systemic bacterial infection in neonates documented by positive blood culture. However, blood culture is positive in only 5.0%–10.0% of suspected cases. Serum concentration of many acute-phase reactants rises in response to infection, which can be used as a non-specific indicator of bacterial sepsis. Aim and Objectives: The aim of this study was to correlate the levels of serum markers C-reactive protein (CRP), serum ferritin, and thrombocytopenia with neonatal sepsis. Materials and Methods: This was a prospective cross-sectional study conducted in the Neonatal Intensive Care Unit, Department of Paediatrics and Pathology, Jawaharlal Nehru Medical College (JNMC), Aligarh from 2019 to 2021 on 172 babies (cases =142; controls = 30). Neonates with sepsis who presented with clinical signs or symptoms of sepsis were taken as case group and healthy neonates served as control. Result: Blood culture was positive in 58 (40.8%) neonates in the case group and Klebsiella was present in maximum number of cases. Blood culture was positive in only 8 (13.8%) cases out of 31 cases of mild thrombocytopenia. The total culture-positive organism was 58 (40.8%), with 09 (15.5%) gram-positive, 46 (79.3%) gram-negative organisms, and 03(5.2%) fungus. Positive CRP was seen in 88 (61.9%) neonates in the case group, out of which, positive culture was noted in 38 (65.5%) neonates and negative in 50 (59.5%) neonates. Serum ferritin values >400 µgm/L was seen in 97 (68.3%) neonates in the case group and 6 (20.0%) neonates in control group. The mean serum ferritin in culture positive neonates was 1024 ± 309 µgm/L and in culture-negative neonates was 999 ± 301 µgm/L. Conclusions: The signs and symptoms of neonatal sepsis are non-specific, leading to difficulty in diagnosis and treatment. Biomarkers such as hematological indices, blood culture, and acute-phase reactants could be more reliable in rapid evaluation and early diagnosis of sepsis and may provide a new diagnostic strategy for the neonates with sepsis.https://journals.lww.com/10.4103/prcm.prcm_11_22c-reactive proteinferritinneonatal sepsisserumthrombocytopenia |
| spellingShingle | Fauzia Talat Kiran Alam Kafil Akhtar S. Monazir Ali A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis Pediatric Respirology and Critical Care Medicine c-reactive protein ferritin neonatal sepsis serum thrombocytopenia |
| title | A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis |
| title_full | A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis |
| title_fullStr | A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis |
| title_full_unstemmed | A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis |
| title_short | A Clinicopathological Study of Thrombocytopenia, Acute-Phase Reactants, and Blood Culture in Neonatal Sepsis |
| title_sort | clinicopathological study of thrombocytopenia acute phase reactants and blood culture in neonatal sepsis |
| topic | c-reactive protein ferritin neonatal sepsis serum thrombocytopenia |
| url | https://journals.lww.com/10.4103/prcm.prcm_11_22 |
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