Functional Characterization of <i>CpPIP1;1</i> and Genome-Wide Analysis of PIPs in Wintersweet (<i>Chimonanthus praecox</i> (L.) Link)

Functional Characterization of <i>CpPIP1;1</i> and Genome-Wide Analysis of PIPs in Wintersweet (<i>Chimonanthus praecox</i> (L.) Link)

Plant aquaporin proteins (AQPs) are categorized into seven distinct families, among which, plasma membrane intrinsic proteins (PIPs) play pivotal roles in plant growth and physiological processes. In this study, we identified 11 <i>CpPIP</i> genes in wintersweet (<i>Chimonanthus pr...

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Main Authors: Fei Ren, Zhu Feng, Guo Wei, Yimeng Lv, Jia Zhao, Yeyuan Deng, Shunzhao Sui, Jing Ma
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Horticulturae
Subjects:
<i>PIP</i> gene family
<i>Chimonanthus praecox</i>
floral development
low-temperature tolerance
Online Access:https://www.mdpi.com/2311-7524/11/6/581
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Summary:Plant aquaporin proteins (AQPs) are categorized into seven distinct families, among which, plasma membrane intrinsic proteins (PIPs) play pivotal roles in plant growth and physiological processes. In this study, we identified 11 <i>CpPIP</i> genes in wintersweet (<i>Chimonanthus praecox</i> (L.) Link) based on bioinformatic characterization of gene structural organization, chromosomal localization, and phylogenetic relationships. Subsequent phylogenetic reconstruction resolved two evolutionarily distinct CpPIP subclasses. We focused on the isolation and characterization of <i>CpPIP1;1</i>, which showed the highest expression in floral organs. During flowering, a significant increase was observed in the expression of the <i>CpPIP1;1</i> gene in response to a gradual reduction in environmental temperature. Moreover, the overexpression of <i>CpPIP1;1</i> in <i>Arabidopsis thaliana</i> resulted in early flowering and an enhanced tolerance to salt, drought, and cold stress. We subsequently transcriptionally fused the <i>CpPIP1;1</i> promoter containing MYC and MYB low-temperature response elements to the <i>β</i>-glucuronidase (<i>GUS</i>) reporter gene and introduced this construct into <i>Nicotiana tabacum</i>. GUS activity assays of the transgenic plants revealed that the <i>CpPIP1;1</i> promoter was effectively expressed in flowers. Furthermore, the promoter transcriptional activity was enhanced in response to salt, drought, cold, gibberellic acid, and methyl jasmonate treatments. Collectively, our findings in this study revealed that <i>CpPIP1;1</i> plays a key role in the regulation of flowering and stress tolerance in wintersweet plants.
ISSN:2311-7524

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