The Root Development Genes (RDGs) Network in <i>Brassica napus</i> and the Role of <i>BnaSHR-6</i> in Response to Low Nitrogen

The Root Development Genes (RDGs) Network in <i>Brassica napus</i> and the Role of <i>BnaSHR-6</i> in Response to Low Nitrogen

The root system is vital for <i>Brassica napus</i> water/nutrient uptake and anchorage, highlighting the importance of identifying root development genes (RDGs). In this study, we identified 218 RDGs in <i>B. napus</i> through homology-based retrieval. Phylogenetic analysis o...

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Main Authors: Xingying Chen, Sining Zhou, Shuang Ye, Zhuo Chen, Zexuan Wu, Shiying Liu, Liping Hu, Xiwen Yang, Xiaoya Yang, Peiji He, Xingzhi Qian, Huafang Wan, Ti Zhang, Nengwen Ying, Huiyan Zhao, Jiana Li, Cunmin Qu, Hai Du
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Plants
Subjects:
<i>Brassica napus</i> L.
root
gene network
evolution
<i>BnaSHR-6</i>
Online Access:https://www.mdpi.com/2223-7747/14/12/1842
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Summary:The root system is vital for <i>Brassica napus</i> water/nutrient uptake and anchorage, highlighting the importance of identifying root development genes (RDGs). In this study, we identified 218 RDGs in <i>B. napus</i> through homology-based retrieval. Phylogenetic analysis of 22 representative species revealed that the RDGs are widely present in plants ranging from aquatic algae to angiosperms. RDGs in <i>B. napus</i> expanded through whole-genome duplication (WGD) events between <i>Brassica rapa</i> and <i>Brassica oleracea</i> ancestors and smaller duplications specific to <i>B. napus</i>. Promoter analysis identified 115 <i>cis</i>-elements, mainly abiotic stress-related and light-responsive. Transcription factor networks showed regulation by BBR-BPC, MIKC_MADS, AP2, and GRAS families. Transcriptome analysis under multiple stresses revealed that low nitrogen (LN) induced the most pronounced changes, with >50% (109/218) of RDGs differentially expressed in roots. Furthermore, we screened the <i>BnaSHR-6</i> gene, which is co-localized in both primary roots (PR) and lateral roots (LR), and responds strongly to LN. Phenotypic analysis revealed that the <i>BnaSHR-6</i> gene regulates the growth and development of both PR and LR under LN conditions, and confers a degree of resistance. These findings advance our understanding of RDGs in <i>B. napus</i> and provide valuable gene resources for subsequent molecular breeding.
ISSN:2223-7747

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