Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue
Objective: In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. Material and Methods: The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) w...
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2016-07-01
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| Series: | Brazilian Dental Science |
| Online Access: | https://ojs.ict.unesp.br/index.php/cob/article/view/1240 |
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| author | João Luiz de Miranda Dhelfeson Willya Douglas de Oliveira Rafael Menezes-Silva Roseana de Almeida Freitas |
| author_facet | João Luiz de Miranda Dhelfeson Willya Douglas de Oliveira Rafael Menezes-Silva Roseana de Almeida Freitas |
| author_sort | João Luiz de Miranda |
| collection | DOAJ |
| description | Objective: In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. Material and Methods: The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) were histopathologically graduated according to Bryne’s method. The immunohistochemical technique utilized specific antibodies to collagen IV and laminin. Histopathologic and immunohistochemical analysis were carried-out on the tumoral invasive front. Results: Most of lower lip carcinomas (91.7%) was classified in lower score and all tongue carcinomas (100%) in high score malignant grade (p<0.01). Collagen type IV expression was absent in the peritumoral basement membrane in 50% of lower lip carcinomas and in 66.7% of tongue carcinomas (p=0.09). Laminin expression was absent in the peritumoral basement membrane in 66.7% of lower lip carcinomas and in 58.3% of tongue carcinomas (p=0.48). When these two glicoproteins were expressed, they showed a linear, thin and discontinuous pattern and a weak intensity of expression. Conclusion: The high score malignancy grade of the tongue carcinomas associated with the expression pattern of the studied matrix proteins. It suggests that tongue squamous cell carcinomas have more invasive potential and more aggressive biological behavior than the lower lip carcinomas.
Keywords
Collagen type IV; Laminin; Carcinoma; Immunohistochemistry.
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| format | Article |
| id | doaj-art-7362c02635eb4525b3ea5d12abdf5bdc |
| institution | Kabale University |
| issn | 2178-6011 |
| language | English |
| publishDate | 2016-07-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Brazilian Dental Science |
| spelling | doaj-art-7362c02635eb4525b3ea5d12abdf5bdc2025-08-22T18:13:08ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112016-07-0119210.14295/bds.2016.v19i2.1240Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongueJoão Luiz de Miranda0Dhelfeson Willya Douglas de Oliveira1Rafael Menezes-Silva2Roseana de Almeida Freitas3Basic Sciences Department – Federal University of Jequitinhonha and Mucuri Valley – Diamantina – MG – Brazil.Post-Graduate Program in Dentistry – Federal University of Minas Gerais – Belo Horizonte – MG – Brazil.Graduate Program in Applied Dental Sciences – Bauru Dental School – University of São Paulo – Bauru – SP – Brazil.Dentistry Department – Federal University of Rio Grande do Norte – Natal – RN – Brazil.Objective: In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. Material and Methods: The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) were histopathologically graduated according to Bryne’s method. The immunohistochemical technique utilized specific antibodies to collagen IV and laminin. Histopathologic and immunohistochemical analysis were carried-out on the tumoral invasive front. Results: Most of lower lip carcinomas (91.7%) was classified in lower score and all tongue carcinomas (100%) in high score malignant grade (p<0.01). Collagen type IV expression was absent in the peritumoral basement membrane in 50% of lower lip carcinomas and in 66.7% of tongue carcinomas (p=0.09). Laminin expression was absent in the peritumoral basement membrane in 66.7% of lower lip carcinomas and in 58.3% of tongue carcinomas (p=0.48). When these two glicoproteins were expressed, they showed a linear, thin and discontinuous pattern and a weak intensity of expression. Conclusion: The high score malignancy grade of the tongue carcinomas associated with the expression pattern of the studied matrix proteins. It suggests that tongue squamous cell carcinomas have more invasive potential and more aggressive biological behavior than the lower lip carcinomas. Keywords Collagen type IV; Laminin; Carcinoma; Immunohistochemistry. https://ojs.ict.unesp.br/index.php/cob/article/view/1240 |
| spellingShingle | João Luiz de Miranda Dhelfeson Willya Douglas de Oliveira Rafael Menezes-Silva Roseana de Almeida Freitas Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue Brazilian Dental Science |
| title | Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue |
| title_full | Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue |
| title_fullStr | Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue |
| title_full_unstemmed | Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue |
| title_short | Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue |
| title_sort | collagen iv and laminin expression in squamous cell carcinomas of lower lip and tongue |
| url | https://ojs.ict.unesp.br/index.php/cob/article/view/1240 |
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