Antibiotic resistance patterns of co-isolated Escherichia coli and Klebsiella spp.: a cross-sectional study on their association with antibiotic usage intensity
Antimicrobial Resistance (AMR) in Escherichia coli (E. coli) and Klebsiella spp. presents a growing public health concern, particularly in low-resource developing countries. However, data on their concurrent resistance patterns within the same host remain limited. This study aims to address this gap...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2025-05-01
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| Series: | Journal of Biological Research |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/jbr/article/view/13803 |
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| Summary: | Antimicrobial Resistance (AMR) in Escherichia coli (E. coli) and Klebsiella spp. presents a growing public health concern, particularly in low-resource developing countries. However, data on their concurrent resistance patterns within the same host remain limited. This study aims to address this gap by evaluating the resistance profiles of co-isolated strains. In the context of this cross-sectional study E. coli and Klebsiella spp. were isolated from stool samples of 138 individuals attending Smart Health Tower, in Iraq. Samples were processed using standard microbiological protocols and identified through Gram staining and biochemical tests. Antibiotic susceptibility was tested using the Kirby-Bauer disk diffusion method. Participants were categorized into low, moderate, and high antibiotic users based on their antibiotic usage patterns. Statistical analysis was performed using SPSS, with significance set at p<0.05. Among E. coli isolates, multidrug resistance was significantly more prevalent in high antibiotic users, with 38/46 (82.6%) exhibiting resistance, compared to 17/46 (37.0%) in moderate antibiotic users and 2/46 (4.3%) in low antibiotic users (p < 0.001). For Klebsiella spp. isolates, multidrug resistance was found in 36/46 (78.3%) of high antibiotic users. Klebsiella spp. showed higher resistance to Ampicillin-Sulbactam (59.9%) compared to E. coli (40.0%). Additionally, resistance to Gentamicin was more pronounced in Klebsiella spp. (80.0%) than in E. coli (20.0%). This study emphasizes the need for stricter antimicrobial stewardship in high antibiotic-use settings. The high multidrug resistance in E. coli and especially Klebsiella spp., necessitates targeted interventions to curb resistance and preserve antimicrobial efficacy.
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| ISSN: | 1826-8838 2284-0230 |