OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice
BackgroundRice blast disease, caused by the fungal pathogen Magnaporthe oryzae, stands as the most destructive diseaset of rice, negatively impacting yield and quality. Identification of blast resistance genes is crucial for breeding disease resistant varieties. In this study, we conducted a genome-...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1629283/full |
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| author | Tianze Ma Mengge Wang Shupeng Xie Shupeng Xie Chengxin Li Jingguo Wang Hualong Liu Luomiao Yang Longnan Men Zhonghua Sun Tianpeng Zong Detang Zou Hongliang Zheng Wei Xin Wei Xin |
| author_facet | Tianze Ma Mengge Wang Shupeng Xie Shupeng Xie Chengxin Li Jingguo Wang Hualong Liu Luomiao Yang Longnan Men Zhonghua Sun Tianpeng Zong Detang Zou Hongliang Zheng Wei Xin Wei Xin |
| author_sort | Tianze Ma |
| collection | DOAJ |
| description | BackgroundRice blast disease, caused by the fungal pathogen Magnaporthe oryzae, stands as the most destructive diseaset of rice, negatively impacting yield and quality. Identification of blast resistance genes is crucial for breeding disease resistant varieties. In this study, we conducted a genome-wide association analysis on rice blast with 295 Japonica rice varieties and the rice blast dominant physiological race ZD5 from Heilongjiang.ResultsA total of 11 Quantitative Trait Loci (QTLs) encompassing 233 genes were mapped. Notably, 40 genes showed significant phenotypic variations among different haplotypes. By combining GWAS and RNA sequencing analysis, we identified five candidate genes related to rice seedling resistance to rice blast. Through the assessment of relative gene expression levels in 10 susceptible and 10 resistant varieties, OsLB2.2 (LOC_Os02g57470) emerged as a key gene displaying significant expression differences in resistant varieties under uninoculated and inoculated conditions. To elucidate gene functionality, we generated oslb2.2 knockout mutants. The results showed that oslb2.2 knockout mutants were significantly enhanced rice blast resistance compared to the wild type. Furthermore, we identified the dominant haplotype of OsLB2.2 and developed Kompetitive Allele - Specific PCR (KASP) molecular markers for molecular improvement of rice blast resistance.ConclusionThe phenotypic validation indicated that OsLB2.2 negatively regulated the rice blast resistance at the seedling stage. Moreover, KASP molecular markers were developed, providing a theoretical basis for the potential application of OsLB2.2 in molecular breeding strategies. |
| format | Article |
| id | doaj-art-735606a492504f2baee30c3604d7e4af |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-735606a492504f2baee30c3604d7e4af2025-08-20T03:40:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.16292831629283OsLB2.2 negatively regulates rice disease resistance at seedling stage in riceTianze Ma0Mengge Wang1Shupeng Xie2Shupeng Xie3Chengxin Li4Jingguo Wang5Hualong Liu6Luomiao Yang7Longnan Men8Zhonghua Sun9Tianpeng Zong10Detang Zou11Hongliang Zheng12Wei Xin13Wei Xin14Key Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaHeilongjiang Academy of Agricultural Sciences, Suihua, ChinaHarbin Academy of Agricultural Sciences, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaHeilongjiang Academy of Agricultural Sciences, Suihua, ChinaHeilongjiang Academy of Agricultural Sciences, Suihua, ChinaHeilongjiang Academy of Agricultural Sciences, Suihua, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, ChinaTianjin Tianlong Science & Technology Company Limited, Tianjin, ChinaBackgroundRice blast disease, caused by the fungal pathogen Magnaporthe oryzae, stands as the most destructive diseaset of rice, negatively impacting yield and quality. Identification of blast resistance genes is crucial for breeding disease resistant varieties. In this study, we conducted a genome-wide association analysis on rice blast with 295 Japonica rice varieties and the rice blast dominant physiological race ZD5 from Heilongjiang.ResultsA total of 11 Quantitative Trait Loci (QTLs) encompassing 233 genes were mapped. Notably, 40 genes showed significant phenotypic variations among different haplotypes. By combining GWAS and RNA sequencing analysis, we identified five candidate genes related to rice seedling resistance to rice blast. Through the assessment of relative gene expression levels in 10 susceptible and 10 resistant varieties, OsLB2.2 (LOC_Os02g57470) emerged as a key gene displaying significant expression differences in resistant varieties under uninoculated and inoculated conditions. To elucidate gene functionality, we generated oslb2.2 knockout mutants. The results showed that oslb2.2 knockout mutants were significantly enhanced rice blast resistance compared to the wild type. Furthermore, we identified the dominant haplotype of OsLB2.2 and developed Kompetitive Allele - Specific PCR (KASP) molecular markers for molecular improvement of rice blast resistance.ConclusionThe phenotypic validation indicated that OsLB2.2 negatively regulated the rice blast resistance at the seedling stage. Moreover, KASP molecular markers were developed, providing a theoretical basis for the potential application of OsLB2.2 in molecular breeding strategies.https://www.frontiersin.org/articles/10.3389/fpls.2025.1629283/fullriceblastgenome-wide association studyRNA-seqresistance gene |
| spellingShingle | Tianze Ma Mengge Wang Shupeng Xie Shupeng Xie Chengxin Li Jingguo Wang Hualong Liu Luomiao Yang Longnan Men Zhonghua Sun Tianpeng Zong Detang Zou Hongliang Zheng Wei Xin Wei Xin OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice Frontiers in Plant Science rice blast genome-wide association study RNA-seq resistance gene |
| title | OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice |
| title_full | OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice |
| title_fullStr | OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice |
| title_full_unstemmed | OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice |
| title_short | OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice |
| title_sort | oslb2 2 negatively regulates rice disease resistance at seedling stage in rice |
| topic | rice blast genome-wide association study RNA-seq resistance gene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1629283/full |
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