Research on Reflective High-Speed Multi-Point Confocal Microscopy System
Reflective confocal imaging systems often face challenges in achieving high resolution, imaging speed, and uniformity, particularly for reflective sample imaging. This study introduces a reflective multi-point confocal imaging system designed to improve imaging speed, expand the field of view, and o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/12/1/22 |
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| author | Huiqin Hu Ning He Kuangwei Du Yukun Zhu Dakai Zhou Yuan Mi Yunbo Chen Junteng Ma Youhua Chen Cuifang Kuang |
| author_facet | Huiqin Hu Ning He Kuangwei Du Yukun Zhu Dakai Zhou Yuan Mi Yunbo Chen Junteng Ma Youhua Chen Cuifang Kuang |
| author_sort | Huiqin Hu |
| collection | DOAJ |
| description | Reflective confocal imaging systems often face challenges in achieving high resolution, imaging speed, and uniformity, particularly for reflective sample imaging. This study introduces a reflective multi-point confocal imaging system designed to improve imaging speed, expand the field of view, and optimize performance for reflective sample imaging using incoherent, telecentric light-emitting diode (LED) illumination. By integrating uniform illumination with microlens arrays and optimizing instantaneous structured illumination microscopy optical components, the system achieves a resolution of 1116 lp/mm (20×, 0.42), 100 fps imaging, and a 500 µm × 500 µm field of view while improving contrast. A comparative analysis of laser- and LED-based scanning demonstrates that incoherent illumination provides superior uniformity and reduces intensity variance in reflective imaging. Validation through photon-reassignment experiments, resolution target imaging, and 100 nm metal particle visualization confirmed the system’s enhanced resolution. Furthermore, ex vivo corneal imaging revealed cellular-level details, suggesting its potential for noncontact corneal diagnostics. |
| format | Article |
| id | doaj-art-7353b56e9ff34f3fb34a0e983777243f |
| institution | Kabale University |
| issn | 2304-6732 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj-art-7353b56e9ff34f3fb34a0e983777243f2025-01-24T13:46:13ZengMDPI AGPhotonics2304-67322024-12-011212210.3390/photonics12010022Research on Reflective High-Speed Multi-Point Confocal Microscopy SystemHuiqin Hu0Ning He1Kuangwei Du2Yukun Zhu3Dakai Zhou4Yuan Mi5Yunbo Chen6Junteng Ma7Youhua Chen8Cuifang Kuang9Ningbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaNingbo Innovation Center, Zhejiang University, Ningbo 315100, ChinaReflective confocal imaging systems often face challenges in achieving high resolution, imaging speed, and uniformity, particularly for reflective sample imaging. This study introduces a reflective multi-point confocal imaging system designed to improve imaging speed, expand the field of view, and optimize performance for reflective sample imaging using incoherent, telecentric light-emitting diode (LED) illumination. By integrating uniform illumination with microlens arrays and optimizing instantaneous structured illumination microscopy optical components, the system achieves a resolution of 1116 lp/mm (20×, 0.42), 100 fps imaging, and a 500 µm × 500 µm field of view while improving contrast. A comparative analysis of laser- and LED-based scanning demonstrates that incoherent illumination provides superior uniformity and reduces intensity variance in reflective imaging. Validation through photon-reassignment experiments, resolution target imaging, and 100 nm metal particle visualization confirmed the system’s enhanced resolution. Furthermore, ex vivo corneal imaging revealed cellular-level details, suggesting its potential for noncontact corneal diagnostics.https://www.mdpi.com/2304-6732/12/1/22reflective confocal microscopeincoherent illuminationphoton reassignment |
| spellingShingle | Huiqin Hu Ning He Kuangwei Du Yukun Zhu Dakai Zhou Yuan Mi Yunbo Chen Junteng Ma Youhua Chen Cuifang Kuang Research on Reflective High-Speed Multi-Point Confocal Microscopy System Photonics reflective confocal microscope incoherent illumination photon reassignment |
| title | Research on Reflective High-Speed Multi-Point Confocal Microscopy System |
| title_full | Research on Reflective High-Speed Multi-Point Confocal Microscopy System |
| title_fullStr | Research on Reflective High-Speed Multi-Point Confocal Microscopy System |
| title_full_unstemmed | Research on Reflective High-Speed Multi-Point Confocal Microscopy System |
| title_short | Research on Reflective High-Speed Multi-Point Confocal Microscopy System |
| title_sort | research on reflective high speed multi point confocal microscopy system |
| topic | reflective confocal microscope incoherent illumination photon reassignment |
| url | https://www.mdpi.com/2304-6732/12/1/22 |
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