Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation
Placental‐derived stromal‐like cells (PLX‐PAD) have been shown to facilitate muscle tissue recovery after injury and stimulate angiogenesis. This work assesses the impact of PLX‐PAD cells on the vascularization and maturation of engineered skeletal muscle tissue. Specifically, their effects in direc...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2024-11-01
|
| Series: | Small Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/smsc.202400228 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850193752093622272 |
|---|---|
| author | Anna Tsukerman Majd Machour Margarita Shuhmaher Eliana O. Fischer Hagit Shoyhet Orit Bar‐Am Gali Guterman Ram Lior Debbi Dina Safina Shulamit Levenberg |
| author_facet | Anna Tsukerman Majd Machour Margarita Shuhmaher Eliana O. Fischer Hagit Shoyhet Orit Bar‐Am Gali Guterman Ram Lior Debbi Dina Safina Shulamit Levenberg |
| author_sort | Anna Tsukerman |
| collection | DOAJ |
| description | Placental‐derived stromal‐like cells (PLX‐PAD) have been shown to facilitate muscle tissue recovery after injury and stimulate angiogenesis. This work assesses the impact of PLX‐PAD cells on the vascularization and maturation of engineered skeletal muscle tissue. Specifically, their effects in direct co‐culture with endothelial cells, pericytes, and myoblasts seeded within microporous 3D scaffolds are characterized. Additionally, the impact of hypoxic PLX‐PAD cell‐conditioned medium (CM) on vascularization and muscle differentiation of engineered tissue is monitored. Co‐culture of PLX‐PAD with myocytes stimulated myocyte differentiation while PLX‐PAD CM promoted the formation of vascular networks. Implantation of a multi‐culture system of vascularized human skeletal muscle tissue and PLX‐PAD into a rectus abdominal defect in nude mice promoted myocyte differentiation, host vessel penetration, and tissue integration. These findings indicate the ability of placenta‐derived cells to induce the formation of vascularized engineered muscle constructs with potential therapeutic applications. |
| format | Article |
| id | doaj-art-734de8b78e49449f9b0afbfad6684639 |
| institution | OA Journals |
| issn | 2688-4046 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-734de8b78e49449f9b0afbfad66846392025-08-20T02:14:10ZengWiley-VCHSmall Science2688-40462024-11-01411n/an/a10.1002/smsc.202400228Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network MaturationAnna Tsukerman0Majd Machour1Margarita Shuhmaher2Eliana O. Fischer3Hagit Shoyhet4Orit Bar‐Am5Gali Guterman Ram6Lior Debbi7Dina Safina8Shulamit Levenberg9Faculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelFaculty of Biomedical Engineering Technion‐Israel Institute of Technology Haifa 3200003 IsraelPlacental‐derived stromal‐like cells (PLX‐PAD) have been shown to facilitate muscle tissue recovery after injury and stimulate angiogenesis. This work assesses the impact of PLX‐PAD cells on the vascularization and maturation of engineered skeletal muscle tissue. Specifically, their effects in direct co‐culture with endothelial cells, pericytes, and myoblasts seeded within microporous 3D scaffolds are characterized. Additionally, the impact of hypoxic PLX‐PAD cell‐conditioned medium (CM) on vascularization and muscle differentiation of engineered tissue is monitored. Co‐culture of PLX‐PAD with myocytes stimulated myocyte differentiation while PLX‐PAD CM promoted the formation of vascular networks. Implantation of a multi‐culture system of vascularized human skeletal muscle tissue and PLX‐PAD into a rectus abdominal defect in nude mice promoted myocyte differentiation, host vessel penetration, and tissue integration. These findings indicate the ability of placenta‐derived cells to induce the formation of vascularized engineered muscle constructs with potential therapeutic applications.https://doi.org/10.1002/smsc.202400228muscle implantationplacenta‐derived mesenchymal stromal cellsregenerative medicineskeletal muscletissue engineeringvascularization |
| spellingShingle | Anna Tsukerman Majd Machour Margarita Shuhmaher Eliana O. Fischer Hagit Shoyhet Orit Bar‐Am Gali Guterman Ram Lior Debbi Dina Safina Shulamit Levenberg Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation Small Science muscle implantation placenta‐derived mesenchymal stromal cells regenerative medicine skeletal muscle tissue engineering vascularization |
| title | Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation |
| title_full | Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation |
| title_fullStr | Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation |
| title_full_unstemmed | Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation |
| title_short | Placenta‐Derived Mesenchymal Stromal‐Like Cells Promote 3D‐Engineered Muscle Tissue Differentiation and Vessel Network Maturation |
| title_sort | placenta derived mesenchymal stromal like cells promote 3d engineered muscle tissue differentiation and vessel network maturation |
| topic | muscle implantation placenta‐derived mesenchymal stromal cells regenerative medicine skeletal muscle tissue engineering vascularization |
| url | https://doi.org/10.1002/smsc.202400228 |
| work_keys_str_mv | AT annatsukerman placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT majdmachour placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT margaritashuhmaher placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT elianaofischer placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT hagitshoyhet placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT oritbaram placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT galigutermanram placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT liordebbi placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT dinasafina placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation AT shulamitlevenberg placentaderivedmesenchymalstromallikecellspromote3dengineeredmuscletissuedifferentiationandvesselnetworkmaturation |