Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder

Abstract The aim of this study was to advance post-traumatic stress disorder (PTSD) understanding in older adults (48–77 years) by determining if circulating cytokines (IL-1β, IL-2, IL-4, IL-6, IL-12p70, IL17A and TNFα), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (V...

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Main Authors: Bonnie L. Quigley, Nathan Wellington, Jacob M. Levenstein, Megan Dutton, Ana P. Bouças, Grace Forsyth, Cyrana C. Gallay, Maryam Hajishafiee, Ciara Treacy, Jim Lagopoulos, Sophie C. Andrews, Adem T. Can, Daniel F. Hermens
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Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-91840-0
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author Bonnie L. Quigley
Nathan Wellington
Jacob M. Levenstein
Megan Dutton
Ana P. Bouças
Grace Forsyth
Cyrana C. Gallay
Maryam Hajishafiee
Ciara Treacy
Jim Lagopoulos
Sophie C. Andrews
Adem T. Can
Daniel F. Hermens
author_facet Bonnie L. Quigley
Nathan Wellington
Jacob M. Levenstein
Megan Dutton
Ana P. Bouças
Grace Forsyth
Cyrana C. Gallay
Maryam Hajishafiee
Ciara Treacy
Jim Lagopoulos
Sophie C. Andrews
Adem T. Can
Daniel F. Hermens
author_sort Bonnie L. Quigley
collection DOAJ
description Abstract The aim of this study was to advance post-traumatic stress disorder (PTSD) understanding in older adults (48–77 years) by determining if circulating cytokines (IL-1β, IL-2, IL-4, IL-6, IL-12p70, IL17A and TNFα), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF-A) and neuroanatomical brain volumes (grey and white matter, hippocampus, and amygdala) significantly differed in those with versus without PTSD. While none of the tested cytokines showed a significant difference, serum BDNF and VEGF-A levels were found to be significantly higher in the PTSD cohort. The assay used for BDNF quantification was important, with differences in general BDNF detected, but not when pro- and mature BDNF were measured specifically. Additionally, BDNF genotyping revealed a significant difference in Val66Met genotype distribution by PTSD diagnosis, with Val66Met carriers generally having lower circulating levels of BDNF compared to their Val66Val counterparts, regardless of PTSD diagnosis. Neuroanatomically, an all-female subset was examined to find total grey and white matter volumes and left and right hippocampal volumes were significantly smaller in those with PTSD. Collectively, these results show that both novel (VEGF-A) and established targets (BDNF and neuroimaging) may serve as useful biomarkers for older adults with PTSD.
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spelling doaj-art-734d5c65ec834b6084bb75e7e0d5a1102025-08-20T02:16:54ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-025-91840-0Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorderBonnie L. Quigley0Nathan Wellington1Jacob M. Levenstein2Megan Dutton3Ana P. Bouças4Grace Forsyth5Cyrana C. Gallay6Maryam Hajishafiee7Ciara Treacy8Jim Lagopoulos9Sophie C. Andrews10Adem T. Can11Daniel F. Hermens12National PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastThompson Brain and Mind HealthcareNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastNational PTSD Research Centre at the Thompson Institute, University of the Sunshine CoastAbstract The aim of this study was to advance post-traumatic stress disorder (PTSD) understanding in older adults (48–77 years) by determining if circulating cytokines (IL-1β, IL-2, IL-4, IL-6, IL-12p70, IL17A and TNFα), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF-A) and neuroanatomical brain volumes (grey and white matter, hippocampus, and amygdala) significantly differed in those with versus without PTSD. While none of the tested cytokines showed a significant difference, serum BDNF and VEGF-A levels were found to be significantly higher in the PTSD cohort. The assay used for BDNF quantification was important, with differences in general BDNF detected, but not when pro- and mature BDNF were measured specifically. Additionally, BDNF genotyping revealed a significant difference in Val66Met genotype distribution by PTSD diagnosis, with Val66Met carriers generally having lower circulating levels of BDNF compared to their Val66Val counterparts, regardless of PTSD diagnosis. Neuroanatomically, an all-female subset was examined to find total grey and white matter volumes and left and right hippocampal volumes were significantly smaller in those with PTSD. Collectively, these results show that both novel (VEGF-A) and established targets (BDNF and neuroimaging) may serve as useful biomarkers for older adults with PTSD.https://doi.org/10.1038/s41598-025-91840-0PTSDBDNFVal66MetVEGF-ACytokinesHippocampus
spellingShingle Bonnie L. Quigley
Nathan Wellington
Jacob M. Levenstein
Megan Dutton
Ana P. Bouças
Grace Forsyth
Cyrana C. Gallay
Maryam Hajishafiee
Ciara Treacy
Jim Lagopoulos
Sophie C. Andrews
Adem T. Can
Daniel F. Hermens
Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
Scientific Reports
PTSD
BDNF
Val66Met
VEGF-A
Cytokines
Hippocampus
title Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
title_full Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
title_fullStr Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
title_full_unstemmed Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
title_short Circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post-traumatic stress disorder
title_sort circulating biomarkers and neuroanatomical brain structures differ in older adults with and without post traumatic stress disorder
topic PTSD
BDNF
Val66Met
VEGF-A
Cytokines
Hippocampus
url https://doi.org/10.1038/s41598-025-91840-0
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