Role of Methyl thiobutyrate to Botrytis cinerea on cucumber
IntroductionBotrytis cinerea is a major agricultural pathogen that causes significant economic Q7 losses worldwide, affecting various crops, including cucumbers. Developing environmentally sustainable control strategies for this pathogen is crucial. Methyl thiobutyrate (MTB), a small organic molecul...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1551274/full |
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| author | Nv Chang Rui Liu Cuihua Lu Yuqing Lai Qian Xu Yuhong Yang Yan Li Jian Ling Bingyan Xie Wenchao Zhao Zhenchuan Mao Jianlong Zhao |
| author_facet | Nv Chang Rui Liu Cuihua Lu Yuqing Lai Qian Xu Yuhong Yang Yan Li Jian Ling Bingyan Xie Wenchao Zhao Zhenchuan Mao Jianlong Zhao |
| author_sort | Nv Chang |
| collection | DOAJ |
| description | IntroductionBotrytis cinerea is a major agricultural pathogen that causes significant economic Q7 losses worldwide, affecting various crops, including cucumbers. Developing environmentally sustainable control strategies for this pathogen is crucial. Methyl thiobutyrate (MTB), a small organic molecule identified in the volatile organic compounds (VOCs) of biocontrol bacteria, has demonstrated potential in inhibiting B. cinerea both in vitro and in vivo.MethodsIn this study, the efficacy of MTB against cucumber gray mold disease was examined by assessing the in vitro and in vivo activities of MTB against B. cinerea and analyzing the transcriptomic data from MTB-treated cucumber leaves infected with B. cinerea.Results and discussionThis study shows that a 2 mg/mL solution of MTB inhibits B. cinerea growth by 98.6% in vitro. In vivo, MTB effectively reduces B. cinerea infection in cucumbers, alleviates necrotic damage in leaf tissues, and significantly reduces disease severity. Transcriptomic analysis reveals that MTB activates the plant immune responses by modulating key MAPK cascade signaling genes and upregulating basal defense genes, including chitinase, pectinase, and lignin biosynthesis genes. Furthermore, MTB influences the signaling pathways of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), resulting in the upregulation of genes such as peroxidase (POD), phenylalanine ammonia-lyase (PAL), lipoxygenase (LOX), and ethyleneresponsive transcription factors (ERFs). These results demonstrate the potential of MTB as an effective biocontrol agent against B. cinerea and provide valuable insights into its underlying mechanisms of action. |
| format | Article |
| id | doaj-art-733ca835d13541e39589e4475275f01c |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-733ca835d13541e39589e4475275f01c2025-08-20T02:16:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-04-011610.3389/fpls.2025.15512741551274Role of Methyl thiobutyrate to Botrytis cinerea on cucumberNv Chang0Rui Liu1Cuihua Lu2Yuqing Lai3Qian Xu4Yuhong Yang5Yan Li6Jian Ling7Bingyan Xie8Wenchao Zhao9Zhenchuan Mao10Jianlong Zhao11Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaBeijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaIntroductionBotrytis cinerea is a major agricultural pathogen that causes significant economic Q7 losses worldwide, affecting various crops, including cucumbers. Developing environmentally sustainable control strategies for this pathogen is crucial. Methyl thiobutyrate (MTB), a small organic molecule identified in the volatile organic compounds (VOCs) of biocontrol bacteria, has demonstrated potential in inhibiting B. cinerea both in vitro and in vivo.MethodsIn this study, the efficacy of MTB against cucumber gray mold disease was examined by assessing the in vitro and in vivo activities of MTB against B. cinerea and analyzing the transcriptomic data from MTB-treated cucumber leaves infected with B. cinerea.Results and discussionThis study shows that a 2 mg/mL solution of MTB inhibits B. cinerea growth by 98.6% in vitro. In vivo, MTB effectively reduces B. cinerea infection in cucumbers, alleviates necrotic damage in leaf tissues, and significantly reduces disease severity. Transcriptomic analysis reveals that MTB activates the plant immune responses by modulating key MAPK cascade signaling genes and upregulating basal defense genes, including chitinase, pectinase, and lignin biosynthesis genes. Furthermore, MTB influences the signaling pathways of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), resulting in the upregulation of genes such as peroxidase (POD), phenylalanine ammonia-lyase (PAL), lipoxygenase (LOX), and ethyleneresponsive transcription factors (ERFs). These results demonstrate the potential of MTB as an effective biocontrol agent against B. cinerea and provide valuable insights into its underlying mechanisms of action.https://www.frontiersin.org/articles/10.3389/fpls.2025.1551274/fullBotrytis cinereacucumberMethyl thiobutyrateinduced resistancebiocontrol agent |
| spellingShingle | Nv Chang Rui Liu Cuihua Lu Yuqing Lai Qian Xu Yuhong Yang Yan Li Jian Ling Bingyan Xie Wenchao Zhao Zhenchuan Mao Jianlong Zhao Role of Methyl thiobutyrate to Botrytis cinerea on cucumber Frontiers in Plant Science Botrytis cinerea cucumber Methyl thiobutyrate induced resistance biocontrol agent |
| title | Role of Methyl thiobutyrate to Botrytis cinerea on cucumber |
| title_full | Role of Methyl thiobutyrate to Botrytis cinerea on cucumber |
| title_fullStr | Role of Methyl thiobutyrate to Botrytis cinerea on cucumber |
| title_full_unstemmed | Role of Methyl thiobutyrate to Botrytis cinerea on cucumber |
| title_short | Role of Methyl thiobutyrate to Botrytis cinerea on cucumber |
| title_sort | role of methyl thiobutyrate to botrytis cinerea on cucumber |
| topic | Botrytis cinerea cucumber Methyl thiobutyrate induced resistance biocontrol agent |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1551274/full |
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