Cloning and analysis of eight tomato ACE full-length cDNA sequences
Previously we have cloned 278 cDNA fragments of ACE genes that exhibit differential expression before and after the Avr/Cf-dependent hypersensitive response occurs. To further analysis the function of these ACE genes in disease resistance, full-length cDNA sequences of 8 ACE fragments, including ACE...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.003 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Previously we have cloned 278 cDNA fragments of ACE genes that exhibit differential expression before and after the Avr/Cf-dependent hypersensitive response occurs. To further analysis the function of these ACE genes in disease resistance, full-length cDNA sequences of 8 ACE fragments, including ACE13, ACE14, ACE19, ACE25, ACE29, ACE36, ACE39 and ACE112, were cloned by employing RACE technique. ACE14, ACE25, ACE29, ACE36 and ACE39 were involved in signal transduction, defence response, protein synthesis and metabolism, while the function of the remaining three was still unknown. Alignment analysis between these ACE sequences and the corresponding sequences deposited in databases revealed that ACE13, ACE25, ACE29, ACE39 and ACE112 were almost identical to their corresponding sequences deposited in databases, while ACE14, ACE19 and ACE36 were significantly different from their mostly matched sequences. ACE14 had an extra extension sequence of 322 bp at 3 prime, and ACE19 showed different 3 sequences of 200 bp, while ACE36 owned an insertion of 132 bp at 5 prime. These results demonstrate that when the EST resources are exploited, care should be taken on the possibility of incorrectness of EST sequences and formation of various products by alternative splicing of one sequence. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |