Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform
Abstract Background Plasma biomarkers for Alzheimer's disease (AD) are a promising tool for accessible and accurate biological diagnostics. However, data in clinical practice are needed to better understand their diagnostic and prognostic ability in memory unit patients. Methods We analyzed pla...
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BMC
2025-03-01
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| Series: | Alzheimer’s Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13195-025-01719-5 |
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| author | Francisco Martínez-Dubarbie Armando Guerra-Ruiz Sara López-García Carmen Lage Marta Fernández-Matarrubia Álvaro Nevado-Cáceres María Rivera-Sánchez Andrea Valera-Barrero Ana Pozueta-Cantudo María García-Martínez Andrea Corrales-Pardo María Bravo Marcos López-Hoyos Juan Irure-Ventura Enrique Marco de Lucas Marta Drake-Pérez Nancy Heidy Cahuana-Santamaría María Teresa García-Unzueta Pascual Sánchez-Juan Eloy Rodríguez-Rodríguez |
| author_facet | Francisco Martínez-Dubarbie Armando Guerra-Ruiz Sara López-García Carmen Lage Marta Fernández-Matarrubia Álvaro Nevado-Cáceres María Rivera-Sánchez Andrea Valera-Barrero Ana Pozueta-Cantudo María García-Martínez Andrea Corrales-Pardo María Bravo Marcos López-Hoyos Juan Irure-Ventura Enrique Marco de Lucas Marta Drake-Pérez Nancy Heidy Cahuana-Santamaría María Teresa García-Unzueta Pascual Sánchez-Juan Eloy Rodríguez-Rodríguez |
| author_sort | Francisco Martínez-Dubarbie |
| collection | DOAJ |
| description | Abstract Background Plasma biomarkers for Alzheimer's disease (AD) are a promising tool for accessible and accurate biological diagnostics. However, data in clinical practice are needed to better understand their diagnostic and prognostic ability in memory unit patients. Methods We analyzed plasma phosphorylated tau at threonine 217 (p-tau217) and neuroflament light chain (NfL) levels and AD cerebrospinal fluid (CSF) biomarkers in a group of 493 subjects using the Lumipulse G600II platform. The sample includes 340 patients from our memory unit (142 dementia, 186 mild cognitive impairment, and 12 with subjective complaints) and 153 cognitively unimpaired volunteers. We have correlated plasma and CSF biomarkers; we have analyzed plasma biomarker levels as a function of clinical diagnosis, cognitive status and amyloid status. We have also studied the ability of p-tau217 to discriminate between amyloid-positive and -negative subjects according to CSF using receiver operating characteristic curves. Results Plasma p-tau217 correlated significantly with CSF Aβ42/Aβ40 (Rho = -0.75; p-value < 0.001), p-tau181 (r = 0.66; p-value < 0.001), and t-tau (r = 0.59; p-value < 0.001). Plasma NfL correlated with CSF NfL (r = 0.48; p-value < 0.001). By clinical diagnosis, plasma p-tau217 levels showed to be higher in AD patients than in healthy controls (difference = 0.63 pg/ml; p-value < 0.001), FTD (difference = 0.60 pg/ml; p-value < 0.001), and nondegenerative dementias (difference = 0.61 pg/ml; p-value < 0.001). Plasma p-tau217 showed an area under the curve of 0.95 to discriminate between A + and A- subjects (95%CI 0.93–0.97). Conclusion Plasma p-tau217 shows excellent results for detecting amyloid pathology at brain level in a clinical setting with an AUC of 0.95. It is a highly specific marker of AD and increases progressively along the disease continuum. Using plasma p-tau217 as an initial diagnostic tool with cut-offs at sensitivities and specificities of 95 or 97.5% could save between 57.4–84.8% of LP/PETs with diagnostic accuracies of 95–97%. Plasma NfL increases progressively at different cognitive stages. |
| format | Article |
| id | doaj-art-7319eafcafeb4be8ada276bbf391624c |
| institution | OA Journals |
| issn | 1758-9193 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Alzheimer’s Research & Therapy |
| spelling | doaj-art-7319eafcafeb4be8ada276bbf391624c2025-08-20T02:10:12ZengBMCAlzheimer’s Research & Therapy1758-91932025-03-0117111610.1186/s13195-025-01719-5Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platformFrancisco Martínez-Dubarbie0Armando Guerra-Ruiz1Sara López-García2Carmen Lage3Marta Fernández-Matarrubia4Álvaro Nevado-Cáceres5María Rivera-Sánchez6Andrea Valera-Barrero7Ana Pozueta-Cantudo8María García-Martínez9Andrea Corrales-Pardo10María Bravo11Marcos López-Hoyos12Juan Irure-Ventura13Enrique Marco de Lucas14Marta Drake-Pérez15Nancy Heidy Cahuana-Santamaría16María Teresa García-Unzueta17Pascual Sánchez-Juan18Eloy Rodríguez-Rodríguez19Neurology Service, Marqués de Valdecilla University HospitalBiochemistry and Clinical Analysis Department, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalNeurology Service, Marqués de Valdecilla University HospitalInstitute for Research Marqués de Valdecilla (IDIVAL)Institute for Research Marqués de Valdecilla (IDIVAL)Institute for Research Marqués de Valdecilla (IDIVAL)Institute for Research Marqués de Valdecilla (IDIVAL)Biochemistry and Clinical Analysis Department, Marqués de Valdecilla University HospitalInstitute for Research Marqués de Valdecilla (IDIVAL)CIBERNED, Network Center for Biomedical Research in Neurodegenerative Diseases, National Institute of Health Carlos IIINeurology Service, Marqués de Valdecilla University HospitalAbstract Background Plasma biomarkers for Alzheimer's disease (AD) are a promising tool for accessible and accurate biological diagnostics. However, data in clinical practice are needed to better understand their diagnostic and prognostic ability in memory unit patients. Methods We analyzed plasma phosphorylated tau at threonine 217 (p-tau217) and neuroflament light chain (NfL) levels and AD cerebrospinal fluid (CSF) biomarkers in a group of 493 subjects using the Lumipulse G600II platform. The sample includes 340 patients from our memory unit (142 dementia, 186 mild cognitive impairment, and 12 with subjective complaints) and 153 cognitively unimpaired volunteers. We have correlated plasma and CSF biomarkers; we have analyzed plasma biomarker levels as a function of clinical diagnosis, cognitive status and amyloid status. We have also studied the ability of p-tau217 to discriminate between amyloid-positive and -negative subjects according to CSF using receiver operating characteristic curves. Results Plasma p-tau217 correlated significantly with CSF Aβ42/Aβ40 (Rho = -0.75; p-value < 0.001), p-tau181 (r = 0.66; p-value < 0.001), and t-tau (r = 0.59; p-value < 0.001). Plasma NfL correlated with CSF NfL (r = 0.48; p-value < 0.001). By clinical diagnosis, plasma p-tau217 levels showed to be higher in AD patients than in healthy controls (difference = 0.63 pg/ml; p-value < 0.001), FTD (difference = 0.60 pg/ml; p-value < 0.001), and nondegenerative dementias (difference = 0.61 pg/ml; p-value < 0.001). Plasma p-tau217 showed an area under the curve of 0.95 to discriminate between A + and A- subjects (95%CI 0.93–0.97). Conclusion Plasma p-tau217 shows excellent results for detecting amyloid pathology at brain level in a clinical setting with an AUC of 0.95. It is a highly specific marker of AD and increases progressively along the disease continuum. Using plasma p-tau217 as an initial diagnostic tool with cut-offs at sensitivities and specificities of 95 or 97.5% could save between 57.4–84.8% of LP/PETs with diagnostic accuracies of 95–97%. Plasma NfL increases progressively at different cognitive stages.https://doi.org/10.1186/s13195-025-01719-5Plasma p-tau217Alzheimer’s diseaseEarly diagnosisCross-sectionalHealthy controlsBiomarkers |
| spellingShingle | Francisco Martínez-Dubarbie Armando Guerra-Ruiz Sara López-García Carmen Lage Marta Fernández-Matarrubia Álvaro Nevado-Cáceres María Rivera-Sánchez Andrea Valera-Barrero Ana Pozueta-Cantudo María García-Martínez Andrea Corrales-Pardo María Bravo Marcos López-Hoyos Juan Irure-Ventura Enrique Marco de Lucas Marta Drake-Pérez Nancy Heidy Cahuana-Santamaría María Teresa García-Unzueta Pascual Sánchez-Juan Eloy Rodríguez-Rodríguez Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform Alzheimer’s Research & Therapy Plasma p-tau217 Alzheimer’s disease Early diagnosis Cross-sectional Healthy controls Biomarkers |
| title | Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform |
| title_full | Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform |
| title_fullStr | Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform |
| title_full_unstemmed | Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform |
| title_short | Diagnostic performance of plasma p-tau217 in a memory clinic cohort using the Lumipulse automated platform |
| title_sort | diagnostic performance of plasma p tau217 in a memory clinic cohort using the lumipulse automated platform |
| topic | Plasma p-tau217 Alzheimer’s disease Early diagnosis Cross-sectional Healthy controls Biomarkers |
| url | https://doi.org/10.1186/s13195-025-01719-5 |
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