Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia
This study presents the genetic structure of two incompatibility (Inc) groups found in Providencia: the newly discovered IncFIIpPROV114-NR and the newly designated IncpCHS4.1–3. An extensive genomic comparison was performed on all 14 plasmids (three IncFIIpPROV114-NR plasmids and 11 IncpCHS4.1–3 pla...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2512034 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849689325421199360 |
|---|---|
| author | Xiuhui Lu Jiaqi He Yali Zheng Fangzhou Chen Jing Luo Kejiao Ma Fan Yang Peng Wang Dongsheng Zhou Bo Gao Zhe Yin |
| author_facet | Xiuhui Lu Jiaqi He Yali Zheng Fangzhou Chen Jing Luo Kejiao Ma Fan Yang Peng Wang Dongsheng Zhou Bo Gao Zhe Yin |
| author_sort | Xiuhui Lu |
| collection | DOAJ |
| description | This study presents the genetic structure of two incompatibility (Inc) groups found in Providencia: the newly discovered IncFIIpPROV114-NR and the newly designated IncpCHS4.1–3. An extensive genomic comparison was performed on all 14 plasmids (three IncFIIpPROV114-NR plasmids and 11 IncpCHS4.1–3 plasmids) from Providencia, including 12 newly sequenced in this study and two from GenBank. Three IncFIIpPROV114-NR plasmids had similar conserved backbones but differed in accessory modules. The 11 IncpCHS4.1–3 plasmids fell into two groups according to differences in the conserved genes of the plasmid backbone. The accessory modules of 11 IncpCHS4.1–3 plasmids showed significant diversity, indicating numerous gene gains and losses, including in the Tn1696-related region, in Tn7504, in a 17.3-kb sul2 region, and a 63.6-kb blaNDM-1 region. A minimum of 45 obtained antimicrobial resistance genes (ARGs) were identified in 13 of the 14 plasmids, covering resistance to 14 classes of antimicrobials and heavy metals. Five new mobile genetic elements (MGEs) were identified, including In2168, In1790, Tn7500, Tn7501, and Tn7502. Additionally, three MGEs, Tn7499, Tn7503, and Tn7504 were newly designated. These two Inc group plasmids integrate abundant accessory modules that allow them to accumulate and distribute ARGs and improve the survivability of Providencia under the pressure of drug selection. |
| format | Article |
| id | doaj-art-7316bdf11f704d6691b18e3fd1c6d53e |
| institution | DOAJ |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-7316bdf11f704d6691b18e3fd1c6d53e2025-08-20T03:21:42ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2512034Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from ProvidenciaXiuhui Lu0Jiaqi He1Yali Zheng2Fangzhou Chen3Jing Luo4Kejiao Ma5Fan Yang6Peng Wang7Dongsheng Zhou8Bo Gao9Zhe Yin10State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, ChinaThis study presents the genetic structure of two incompatibility (Inc) groups found in Providencia: the newly discovered IncFIIpPROV114-NR and the newly designated IncpCHS4.1–3. An extensive genomic comparison was performed on all 14 plasmids (three IncFIIpPROV114-NR plasmids and 11 IncpCHS4.1–3 plasmids) from Providencia, including 12 newly sequenced in this study and two from GenBank. Three IncFIIpPROV114-NR plasmids had similar conserved backbones but differed in accessory modules. The 11 IncpCHS4.1–3 plasmids fell into two groups according to differences in the conserved genes of the plasmid backbone. The accessory modules of 11 IncpCHS4.1–3 plasmids showed significant diversity, indicating numerous gene gains and losses, including in the Tn1696-related region, in Tn7504, in a 17.3-kb sul2 region, and a 63.6-kb blaNDM-1 region. A minimum of 45 obtained antimicrobial resistance genes (ARGs) were identified in 13 of the 14 plasmids, covering resistance to 14 classes of antimicrobials and heavy metals. Five new mobile genetic elements (MGEs) were identified, including In2168, In1790, Tn7500, Tn7501, and Tn7502. Additionally, three MGEs, Tn7499, Tn7503, and Tn7504 were newly designated. These two Inc group plasmids integrate abundant accessory modules that allow them to accumulate and distribute ARGs and improve the survivability of Providencia under the pressure of drug selection.https://www.tandfonline.com/doi/10.1080/21505594.2025.2512034ProvidenciaIncFIIpPROV114-NR plasmidsIncpCHS4.1-3 plasmidsunit transposonsantimicrobial resistance genes |
| spellingShingle | Xiuhui Lu Jiaqi He Yali Zheng Fangzhou Chen Jing Luo Kejiao Ma Fan Yang Peng Wang Dongsheng Zhou Bo Gao Zhe Yin Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia Virulence Providencia IncFIIpPROV114-NR plasmids IncpCHS4.1-3 plasmids unit transposons antimicrobial resistance genes |
| title | Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia |
| title_full | Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia |
| title_fullStr | Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia |
| title_full_unstemmed | Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia |
| title_short | Plasmids of two novel incompatibility groups IncFIIpPROV114-NR and IncpCHS4.1-3 from Providencia |
| title_sort | plasmids of two novel incompatibility groups incfiipprov114 nr and incpchs4 1 3 from providencia |
| topic | Providencia IncFIIpPROV114-NR plasmids IncpCHS4.1-3 plasmids unit transposons antimicrobial resistance genes |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2512034 |
| work_keys_str_mv | AT xiuhuilu plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT jiaqihe plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT yalizheng plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT fangzhouchen plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT jingluo plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT kejiaoma plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT fanyang plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT pengwang plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT dongshengzhou plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT bogao plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia AT zheyin plasmidsoftwonovelincompatibilitygroupsincfiipprov114nrandincpchs413fromprovidencia |