Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site

Since ancient times, the community of Xochimilco in the south of Mexico City has provided vegetables for the entire city. Today, Lake Xochimilco is listed as a UNESCO World Heritage Site because it is the last remaining bastion of Aztec culture and preserves the extraordinary ecological landscape of...

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Main Authors: Alvaro de Obeso Fernández del Valle, Jorge Membrillo-Hernández
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/4/835
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author Alvaro de Obeso Fernández del Valle
Jorge Membrillo-Hernández
author_facet Alvaro de Obeso Fernández del Valle
Jorge Membrillo-Hernández
author_sort Alvaro de Obeso Fernández del Valle
collection DOAJ
description Since ancient times, the community of Xochimilco in the south of Mexico City has provided vegetables for the entire city. Today, Lake Xochimilco is listed as a UNESCO World Heritage Site because it is the last remaining bastion of Aztec culture and preserves the extraordinary ecological landscape of chinampas, a system of arable islands that has endured for over 1000 years. Here, we report on the microbiological communities currently existing in the lake. This is relevant since the water irrigates crops on the islands, known as chinampas. To achieve this, samples from the lake were collected at two different sites, and metagenomics analysis of the <i>16S</i> gene was conducted. The results indicate the presence of five dominant bacterial phyla: <i>Actinobacteria</i> (44.5%), <i>Proteobacteria</i> (22.5%), <i>Firmicutes</i> (13%), <i>Bacteroidota</i> (6%), and <i>Chloroflexi</i> (4.6%). The most abundant families were <i>Micrococcaceae</i>, <i>Intrasporangiaceae</i>, and <i>Rhodobacteraceae</i>. The results correlate with current anthropogenic activity, indicating a moderate problem associated with contamination. Our findings suggest that immediate actions and increased awareness are necessary to preserve this cultural and natural heritage site and to take steps to comply with Sustainable Development Goal 11 (Sustainable Cities and Communities). Furthermore, this is the first report to characterize microbial communities in the water of Lake Xochimilco using <i>16S</i> rRNA gene sequencing.
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spelling doaj-art-731523bcfbe04b35a67ad1c66b800dbf2025-08-20T03:13:58ZengMDPI AGMicroorganisms2076-26072025-04-0113483510.3390/microorganisms13040835Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage SiteAlvaro de Obeso Fernández del Valle0Jorge Membrillo-Hernández1Bioengineering Department, School of Engineering and Sciences, Tecnologico de Monterrey, Mexico City Campus, Calle del Puente 222, Col. Ejidos de Huipulco, Tlalpan, Mexico City 14380, MexicoBioengineering Department, School of Engineering and Sciences, Tecnologico de Monterrey, Mexico City Campus, Calle del Puente 222, Col. Ejidos de Huipulco, Tlalpan, Mexico City 14380, MexicoSince ancient times, the community of Xochimilco in the south of Mexico City has provided vegetables for the entire city. Today, Lake Xochimilco is listed as a UNESCO World Heritage Site because it is the last remaining bastion of Aztec culture and preserves the extraordinary ecological landscape of chinampas, a system of arable islands that has endured for over 1000 years. Here, we report on the microbiological communities currently existing in the lake. This is relevant since the water irrigates crops on the islands, known as chinampas. To achieve this, samples from the lake were collected at two different sites, and metagenomics analysis of the <i>16S</i> gene was conducted. The results indicate the presence of five dominant bacterial phyla: <i>Actinobacteria</i> (44.5%), <i>Proteobacteria</i> (22.5%), <i>Firmicutes</i> (13%), <i>Bacteroidota</i> (6%), and <i>Chloroflexi</i> (4.6%). The most abundant families were <i>Micrococcaceae</i>, <i>Intrasporangiaceae</i>, and <i>Rhodobacteraceae</i>. The results correlate with current anthropogenic activity, indicating a moderate problem associated with contamination. Our findings suggest that immediate actions and increased awareness are necessary to preserve this cultural and natural heritage site and to take steps to comply with Sustainable Development Goal 11 (Sustainable Cities and Communities). Furthermore, this is the first report to characterize microbial communities in the water of Lake Xochimilco using <i>16S</i> rRNA gene sequencing.https://www.mdpi.com/2076-2607/13/4/835metagenomics<i>16S</i> RNAphylumchinampaSDG11cultural heritage
spellingShingle Alvaro de Obeso Fernández del Valle
Jorge Membrillo-Hernández
Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
Microorganisms
metagenomics
<i>16S</i> RNA
phylum
chinampa
SDG11
cultural heritage
title Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
title_full Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
title_fullStr Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
title_full_unstemmed Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
title_short Metagenomics Analysis of the Microbial Consortium in Samples from Lake Xochimilco, a World Cultural Heritage Site
title_sort metagenomics analysis of the microbial consortium in samples from lake xochimilco a world cultural heritage site
topic metagenomics
<i>16S</i> RNA
phylum
chinampa
SDG11
cultural heritage
url https://www.mdpi.com/2076-2607/13/4/835
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