Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study

Objective: Indonesia commenced the nationwide introduction of pneumococcal conjugate vaccine (PCV) in 2022. Pre-vaccine Streptococcus pneumoniae data from across the country could be critical to enable vaccine impact evaluation in the future. This study evaluates colonization prevalence, factors ass...

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Main Authors: Wa Ode Dwi Daningrat, Wisiva Tofriska Paramaiswari, Hanifah Fajri Maharani Putri, David Aanensen, Dodi Safari
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Journal of Global Antimicrobial Resistance
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213716524001590
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author Wa Ode Dwi Daningrat
Wisiva Tofriska Paramaiswari
Hanifah Fajri Maharani Putri
David Aanensen
Dodi Safari
author_facet Wa Ode Dwi Daningrat
Wisiva Tofriska Paramaiswari
Hanifah Fajri Maharani Putri
David Aanensen
Dodi Safari
author_sort Wa Ode Dwi Daningrat
collection DOAJ
description Objective: Indonesia commenced the nationwide introduction of pneumococcal conjugate vaccine (PCV) in 2022. Pre-vaccine Streptococcus pneumoniae data from across the country could be critical to enable vaccine impact evaluation in the future. This study evaluates colonization prevalence, factors associated with colonization, serotype distribution, and the antimicrobial susceptibility profile of S. pneumoniae. Methods: Children under 5 years of age were enrolled from Bajau tribe settlements in Wakatobi, southeast Sulawesi, Indonesia, from October 2018 to February 2019. Nasopharyngeal swab specimens were analysed by culture, and isolates were serotyped using sequential multiplex polymerase chain reaction. Antibiotic susceptibility was performed by the disk diffusion method. Multivariable logistic regression was performed for risk factor analysis. Results: A total of 499 NP swab specimens were collected; 61.9% were colonized with S. pneumoniae and 48.9% of the isolates were of PCV13-vaccine type. The most common serotypes were 23F, 6B, 19F, and 6A at 13.2%, 9.8%, 8.9%, and 8.0%, respectively. Exposure to cigarette smoke in the household and runny nose were significant risk factors for colonization, with aORs of 1.6 (95% confidence interval: 1.1–2.3) and 2.1 (95% confidence interval: 1.4–3.3), respectively. Conclusions: The findings of this study may contribute to baseline pre-vaccine data in Indonesia that would be critical for the impact evaluation of vaccines.
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spelling doaj-art-730e664f4b46430e9247c6bdff061bd32025-08-20T01:59:47ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-0139939910.1016/j.jgar.2024.08.006Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional studyWa Ode Dwi Daningrat0Wisiva Tofriska Paramaiswari1Hanifah Fajri Maharani Putri2David Aanensen3Dodi Safari4Centre for Genomic Pathogen Surveillance, Nuffield Department of Medicine, University of Oxford, Oxford, UKMaster's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Cluster of Infectious Diseases and Immunology. Indonesian Medical Education and Research Institute (IMERI), IndonesiaCluster of Infectious Diseases and Immunology. Indonesian Medical Education and Research Institute (IMERI), IndonesiaCentre for Genomic Pathogen Surveillance, Nuffield Department of Medicine, University of Oxford, Oxford, UKEijkman Research Centre for Molecular Biology, National Research and Innovation Agency, Cibinong, West Java, Indonesia; Corresponding author. Eijkman Research Centre for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia.Objective: Indonesia commenced the nationwide introduction of pneumococcal conjugate vaccine (PCV) in 2022. Pre-vaccine Streptococcus pneumoniae data from across the country could be critical to enable vaccine impact evaluation in the future. This study evaluates colonization prevalence, factors associated with colonization, serotype distribution, and the antimicrobial susceptibility profile of S. pneumoniae. Methods: Children under 5 years of age were enrolled from Bajau tribe settlements in Wakatobi, southeast Sulawesi, Indonesia, from October 2018 to February 2019. Nasopharyngeal swab specimens were analysed by culture, and isolates were serotyped using sequential multiplex polymerase chain reaction. Antibiotic susceptibility was performed by the disk diffusion method. Multivariable logistic regression was performed for risk factor analysis. Results: A total of 499 NP swab specimens were collected; 61.9% were colonized with S. pneumoniae and 48.9% of the isolates were of PCV13-vaccine type. The most common serotypes were 23F, 6B, 19F, and 6A at 13.2%, 9.8%, 8.9%, and 8.0%, respectively. Exposure to cigarette smoke in the household and runny nose were significant risk factors for colonization, with aORs of 1.6 (95% confidence interval: 1.1–2.3) and 2.1 (95% confidence interval: 1.4–3.3), respectively. Conclusions: The findings of this study may contribute to baseline pre-vaccine data in Indonesia that would be critical for the impact evaluation of vaccines.http://www.sciencedirect.com/science/article/pii/S2213716524001590Streptococcus pneumoniaeNasopharyngeal carriage ratePCV introductionSea NomadsIndonesia
spellingShingle Wa Ode Dwi Daningrat
Wisiva Tofriska Paramaiswari
Hanifah Fajri Maharani Putri
David Aanensen
Dodi Safari
Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
Journal of Global Antimicrobial Resistance
Streptococcus pneumoniae
Nasopharyngeal carriage rate
PCV introduction
Sea Nomads
Indonesia
title Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
title_full Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
title_fullStr Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
title_full_unstemmed Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
title_short Prevalence, serotype distribution, and antimicrobial susceptibility profile of Streptococcus pneumoniae in Sea Nomad children under 5 years of age in Wakatobi, Southeast Sulawesi, Indonesia: A cross-sectional study
title_sort prevalence serotype distribution and antimicrobial susceptibility profile of streptococcus pneumoniae in sea nomad children under 5 years of age in wakatobi southeast sulawesi indonesia a cross sectional study
topic Streptococcus pneumoniae
Nasopharyngeal carriage rate
PCV introduction
Sea Nomads
Indonesia
url http://www.sciencedirect.com/science/article/pii/S2213716524001590
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