Out of Africa: The genomic footprints of Vietnamese Robusta coffee.

Vietnam is the main producer of Robusta (Coffea canephora) coffee, but faces several future agronomic challenges. These may be addressed through breeding for improved cultivars and more sustainable cropping systems. For such efforts to be successful and efficient, locally available genetic resources...

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Main Authors: Tram Vi, Thi Nhu Le, Philippe Cubry, Viet Ha Phan, Thi Tieu Oanh Dinh, Thi Bich Ngoc Tran, Van Toan Nguyen, Claude Patrick Millet, Jean-Léon Kambale, Gba Kossia Manzan Karine, Pascal Musoli, Ucu Sumirat, Jose Cassule Mahinga, Piet Stoffelen, Dapeng Zhang, Pierre Marraccini, Yves Vigouroux, Ngan Giang Khong, Valerie Poncet
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0324988
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author Tram Vi
Thi Nhu Le
Philippe Cubry
Viet Ha Phan
Thi Tieu Oanh Dinh
Thi Bich Ngoc Tran
Van Toan Nguyen
Claude Patrick Millet
Jean-Léon Kambale
Gba Kossia Manzan Karine
Pascal Musoli
Ucu Sumirat
Jose Cassule Mahinga
Piet Stoffelen
Dapeng Zhang
Pierre Marraccini
Yves Vigouroux
Ngan Giang Khong
Valerie Poncet
author_facet Tram Vi
Thi Nhu Le
Philippe Cubry
Viet Ha Phan
Thi Tieu Oanh Dinh
Thi Bich Ngoc Tran
Van Toan Nguyen
Claude Patrick Millet
Jean-Léon Kambale
Gba Kossia Manzan Karine
Pascal Musoli
Ucu Sumirat
Jose Cassule Mahinga
Piet Stoffelen
Dapeng Zhang
Pierre Marraccini
Yves Vigouroux
Ngan Giang Khong
Valerie Poncet
author_sort Tram Vi
collection DOAJ
description Vietnam is the main producer of Robusta (Coffea canephora) coffee, but faces several future agronomic challenges. These may be addressed through breeding for improved cultivars and more sustainable cropping systems. For such efforts to be successful and efficient, locally available genetic resources must be understood. Indeed, while C. canephora exhibits high genetic diversity in its native tropical African forests, only a part of it contributed to the worldwide diffusion of Robusta. Here we traced the African origins of Robusta accessions cultivated in the Central Highlands of Vietnam. A total of 126 Robusta accessions from the Vietnam coffee germplasm collection were characterized, including historical, elite and local cultivated clones. Their genetic diversity and origins were inferred through comparisons with wild reference samples using a new set of 261 genome-wide SNPs. A core set of 45 accessions that maximize the genetic distance and allelic richness were identified for conservation and breeding priorities. Full genome sequencing of these individuals helped to closely trace the origins of chromosomal segments back to different, geographically-structured wild African genetic groups. All Vietnamese Robusta accessions displayed Congo Basin (ER group) origins, albeit to various extents. However, we also uncovered contribution from several other genetic groups, variously from the Guinean region (D), the central African Atlantic coast (AG), and Eastern CAR/Uganda (OB), in 31 hybrid individuals. These source groups have been widely used in crossbreeding to develop elite clones. In addition, using whole-genome sequencing data, we also identified various admixture patterns at the chromosome level among the hybrids, which might provide valuable information for selecting breeding materials.
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spelling doaj-art-730e3a4e6c4443bc99fe08bb7e5455942025-08-20T02:33:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01205e032498810.1371/journal.pone.0324988Out of Africa: The genomic footprints of Vietnamese Robusta coffee.Tram ViThi Nhu LePhilippe CubryViet Ha PhanThi Tieu Oanh DinhThi Bich Ngoc TranVan Toan NguyenClaude Patrick MilletJean-Léon KambaleGba Kossia Manzan KarinePascal MusoliUcu SumiratJose Cassule MahingaPiet StoffelenDapeng ZhangPierre MarracciniYves VigourouxNgan Giang KhongValerie PoncetVietnam is the main producer of Robusta (Coffea canephora) coffee, but faces several future agronomic challenges. These may be addressed through breeding for improved cultivars and more sustainable cropping systems. For such efforts to be successful and efficient, locally available genetic resources must be understood. Indeed, while C. canephora exhibits high genetic diversity in its native tropical African forests, only a part of it contributed to the worldwide diffusion of Robusta. Here we traced the African origins of Robusta accessions cultivated in the Central Highlands of Vietnam. A total of 126 Robusta accessions from the Vietnam coffee germplasm collection were characterized, including historical, elite and local cultivated clones. Their genetic diversity and origins were inferred through comparisons with wild reference samples using a new set of 261 genome-wide SNPs. A core set of 45 accessions that maximize the genetic distance and allelic richness were identified for conservation and breeding priorities. Full genome sequencing of these individuals helped to closely trace the origins of chromosomal segments back to different, geographically-structured wild African genetic groups. All Vietnamese Robusta accessions displayed Congo Basin (ER group) origins, albeit to various extents. However, we also uncovered contribution from several other genetic groups, variously from the Guinean region (D), the central African Atlantic coast (AG), and Eastern CAR/Uganda (OB), in 31 hybrid individuals. These source groups have been widely used in crossbreeding to develop elite clones. In addition, using whole-genome sequencing data, we also identified various admixture patterns at the chromosome level among the hybrids, which might provide valuable information for selecting breeding materials.https://doi.org/10.1371/journal.pone.0324988
spellingShingle Tram Vi
Thi Nhu Le
Philippe Cubry
Viet Ha Phan
Thi Tieu Oanh Dinh
Thi Bich Ngoc Tran
Van Toan Nguyen
Claude Patrick Millet
Jean-Léon Kambale
Gba Kossia Manzan Karine
Pascal Musoli
Ucu Sumirat
Jose Cassule Mahinga
Piet Stoffelen
Dapeng Zhang
Pierre Marraccini
Yves Vigouroux
Ngan Giang Khong
Valerie Poncet
Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
PLoS ONE
title Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
title_full Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
title_fullStr Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
title_full_unstemmed Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
title_short Out of Africa: The genomic footprints of Vietnamese Robusta coffee.
title_sort out of africa the genomic footprints of vietnamese robusta coffee
url https://doi.org/10.1371/journal.pone.0324988
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