Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance

Abstract Human‐induced climate change, land use changes, and urbanization are predicted to dramatically impact landscape hydrology, which can have devastating impacts on aquatic organisms. For amphibians that rely on aquatic environments to breed and develop, it is essential to understand how the la...

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Main Authors: Laura A. Brannelly, Michel E. B. Ohmer, Lydia Zimmerman, Trina M. Wantman, Phoebe L. Reuben, Jakub Zegar, Samantha S. Fontaine, Molly C. Bletz, Brandon C. LaBumbard, Matthew D. Venesky, Rebecca L. Cramp, Douglas C. Woodhams, Corinne L. Richards‐Zawacki
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Ecosphere
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Online Access:https://doi.org/10.1002/ecs2.70177
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author Laura A. Brannelly
Michel E. B. Ohmer
Lydia Zimmerman
Trina M. Wantman
Phoebe L. Reuben
Jakub Zegar
Samantha S. Fontaine
Molly C. Bletz
Brandon C. LaBumbard
Matthew D. Venesky
Rebecca L. Cramp
Douglas C. Woodhams
Corinne L. Richards‐Zawacki
author_facet Laura A. Brannelly
Michel E. B. Ohmer
Lydia Zimmerman
Trina M. Wantman
Phoebe L. Reuben
Jakub Zegar
Samantha S. Fontaine
Molly C. Bletz
Brandon C. LaBumbard
Matthew D. Venesky
Rebecca L. Cramp
Douglas C. Woodhams
Corinne L. Richards‐Zawacki
author_sort Laura A. Brannelly
collection DOAJ
description Abstract Human‐induced climate change, land use changes, and urbanization are predicted to dramatically impact landscape hydrology, which can have devastating impacts on aquatic organisms. For amphibians that rely on aquatic environments to breed and develop, it is essential to understand how the larval environment impacts development, condition, and performance later in life. Two important predicted impacts of climate change, urbanization, and land use changes are reduced hydroperiod and variable larval density. Here, we explored how larval density and hydroperiod affect development, morphology, physiology, and immune defenses at metamorphosis and 35 days post‐metamorphosis in the frog Rana pipiens. We found that high‐density larval conditions had a large negative impact on development and morphology, which resulted in longer larval periods, reduced likelihood of metamorphosis, smaller size at metamorphosis, shorter femur to body length ratio, and reduced microbiome species evenness compared with animals that developed in low‐density conditions. However, animals from the high‐density treatment experienced compensatory growth post‐metamorphosis, demonstrating accelerated growth in body size and relative femur length compared with animals from the low‐density treatments, despite not “catching‐up” in size. We also observed an increase in relative gut length and relative liver size in animals that had developed in the high‐density treatment than those in the low‐density treatment, as well as higher bacterial killing ability, and greater jump distances relative to their leg length across different temperatures. Finally, metabolic rate was higher overall but especially at higher test temperatures for animals that developed under high‐density conditions, indicating that these animals may expend more energy in response to acute temperature changes. While the effects of climate change have direct negative effects on larval development and metamorphosis, animals can increase growth rate post‐metamorphosis; however, that compensatory growth might come at a cost and reduce their ability to cope with further environmental change such as increased temperatures.
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spelling doaj-art-7305826b41b74d768dcb0da8bc6517732025-08-20T02:03:43ZengWileyEcosphere2150-89252025-02-01162n/an/a10.1002/ecs2.70177Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performanceLaura A. Brannelly0Michel E. B. Ohmer1Lydia Zimmerman2Trina M. Wantman3Phoebe L. Reuben4Jakub Zegar5Samantha S. Fontaine6Molly C. Bletz7Brandon C. LaBumbard8Matthew D. Venesky9Rebecca L. Cramp10Douglas C. Woodhams11Corinne L. Richards‐Zawacki12Melbourne Veterinary School, Faculty of Science University of Melbourne Werribee Victoria AustraliaDepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Biology University of Massachusetts Boston Boston Massachusetts USADepartment of Biology University of Massachusetts Boston Boston Massachusetts USADepartment of Biology Allegheny College Meadville Pennsylvania USASchool of the Environment University of Queensland Brisbane Queensland AustraliaDepartment of Biology University of Massachusetts Boston Boston Massachusetts USADepartment of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USAAbstract Human‐induced climate change, land use changes, and urbanization are predicted to dramatically impact landscape hydrology, which can have devastating impacts on aquatic organisms. For amphibians that rely on aquatic environments to breed and develop, it is essential to understand how the larval environment impacts development, condition, and performance later in life. Two important predicted impacts of climate change, urbanization, and land use changes are reduced hydroperiod and variable larval density. Here, we explored how larval density and hydroperiod affect development, morphology, physiology, and immune defenses at metamorphosis and 35 days post‐metamorphosis in the frog Rana pipiens. We found that high‐density larval conditions had a large negative impact on development and morphology, which resulted in longer larval periods, reduced likelihood of metamorphosis, smaller size at metamorphosis, shorter femur to body length ratio, and reduced microbiome species evenness compared with animals that developed in low‐density conditions. However, animals from the high‐density treatment experienced compensatory growth post‐metamorphosis, demonstrating accelerated growth in body size and relative femur length compared with animals from the low‐density treatments, despite not “catching‐up” in size. We also observed an increase in relative gut length and relative liver size in animals that had developed in the high‐density treatment than those in the low‐density treatment, as well as higher bacterial killing ability, and greater jump distances relative to their leg length across different temperatures. Finally, metabolic rate was higher overall but especially at higher test temperatures for animals that developed under high‐density conditions, indicating that these animals may expend more energy in response to acute temperature changes. While the effects of climate change have direct negative effects on larval development and metamorphosis, animals can increase growth rate post‐metamorphosis; however, that compensatory growth might come at a cost and reduce their ability to cope with further environmental change such as increased temperatures.https://doi.org/10.1002/ecs2.70177amphibiancompensatory growthdensitydevelopmental plasticityhydroperiodimmunity
spellingShingle Laura A. Brannelly
Michel E. B. Ohmer
Lydia Zimmerman
Trina M. Wantman
Phoebe L. Reuben
Jakub Zegar
Samantha S. Fontaine
Molly C. Bletz
Brandon C. LaBumbard
Matthew D. Venesky
Rebecca L. Cramp
Douglas C. Woodhams
Corinne L. Richards‐Zawacki
Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
Ecosphere
amphibian
compensatory growth
density
developmental plasticity
hydroperiod
immunity
title Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
title_full Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
title_fullStr Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
title_full_unstemmed Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
title_short Post‐metamorphic growth partially compensates for the effects of climate‐driven stressors on juvenile frog performance
title_sort post metamorphic growth partially compensates for the effects of climate driven stressors on juvenile frog performance
topic amphibian
compensatory growth
density
developmental plasticity
hydroperiod
immunity
url https://doi.org/10.1002/ecs2.70177
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