Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2.
Senescence of vascular endothelial cells leads to endothelial dysfunction and exacerbates atherosclerosis. In this study, we presented evidence that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-Exos) could delay endothelial cell senescence, promote endothelial cell proli...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0327173 |
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| author | Zhi-Peng Yang Shui-Hong Lu Yan-Hong Pan Zhao-Fu Liao Yi-Tuan Xie Heng Li Yu-Lan Zhou Zhen-Can Shi Yun-Fei Qu Zhu-Guo Wu Chongxiang Xiong Xing-Dong Xiong |
| author_facet | Zhi-Peng Yang Shui-Hong Lu Yan-Hong Pan Zhao-Fu Liao Yi-Tuan Xie Heng Li Yu-Lan Zhou Zhen-Can Shi Yun-Fei Qu Zhu-Guo Wu Chongxiang Xiong Xing-Dong Xiong |
| author_sort | Zhi-Peng Yang |
| collection | DOAJ |
| description | Senescence of vascular endothelial cells leads to endothelial dysfunction and exacerbates atherosclerosis. In this study, we presented evidence that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-Exos) could delay endothelial cell senescence, promote endothelial cell proliferation, and enhance angiogenic activity in vitro. The miRNA profiling analysis revealed a high expression of miR-143-3p in hucMSC-Exos, which was further upregulated in endothelial cells treated with hucMSC-Exos. Silencing miR-143-3p induced endothelial cell senescence, as evidenced by increased senescence-associated β-galactosidase activity, reduced cell proliferation, and inhibited tubular formation; conversely, overexpression of miR-143-3p exhibited opposite effects. Moreover, we found that miR-143-3p directly targeted Cyclooxygenase-2 (COX-2) and suppressed its translation, thus delaying endothelial cell senescence. These results suggested that hucMSC-Exos can delay endothelial cell senescence by transferring miR-143-3p. In summary, our data demonstrated the potential of hucMSC-Exos as an intervention against vascular aging. |
| format | Article |
| id | doaj-art-7300676cdfc848daa1db9ae2b735527b |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7300676cdfc848daa1db9ae2b735527b2025-08-20T03:13:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032717310.1371/journal.pone.0327173Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2.Zhi-Peng YangShui-Hong LuYan-Hong PanZhao-Fu LiaoYi-Tuan XieHeng LiYu-Lan ZhouZhen-Can ShiYun-Fei QuZhu-Guo WuChongxiang XiongXing-Dong XiongSenescence of vascular endothelial cells leads to endothelial dysfunction and exacerbates atherosclerosis. In this study, we presented evidence that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-Exos) could delay endothelial cell senescence, promote endothelial cell proliferation, and enhance angiogenic activity in vitro. The miRNA profiling analysis revealed a high expression of miR-143-3p in hucMSC-Exos, which was further upregulated in endothelial cells treated with hucMSC-Exos. Silencing miR-143-3p induced endothelial cell senescence, as evidenced by increased senescence-associated β-galactosidase activity, reduced cell proliferation, and inhibited tubular formation; conversely, overexpression of miR-143-3p exhibited opposite effects. Moreover, we found that miR-143-3p directly targeted Cyclooxygenase-2 (COX-2) and suppressed its translation, thus delaying endothelial cell senescence. These results suggested that hucMSC-Exos can delay endothelial cell senescence by transferring miR-143-3p. In summary, our data demonstrated the potential of hucMSC-Exos as an intervention against vascular aging.https://doi.org/10.1371/journal.pone.0327173 |
| spellingShingle | Zhi-Peng Yang Shui-Hong Lu Yan-Hong Pan Zhao-Fu Liao Yi-Tuan Xie Heng Li Yu-Lan Zhou Zhen-Can Shi Yun-Fei Qu Zhu-Guo Wu Chongxiang Xiong Xing-Dong Xiong Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. PLoS ONE |
| title | Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. |
| title_full | Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. |
| title_fullStr | Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. |
| title_full_unstemmed | Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. |
| title_short | Umbilical cord mesenchymal stem cell exosomal miR-143-3p delays endothelial cell senescence through targeting COX-2. |
| title_sort | umbilical cord mesenchymal stem cell exosomal mir 143 3p delays endothelial cell senescence through targeting cox 2 |
| url | https://doi.org/10.1371/journal.pone.0327173 |
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