Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection
Nickel modified chitosan nanoparticles are promising catalysts for the determination of bioanalytes such as insulin, glucose, antibiotics, and ascorbic acid. In this study, we synthesized nickel-loaded chitosan nanoparticles to evaluate their potential as surface modifiers for electrochemical sensor...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Biosensors and Bioelectronics: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000512 |
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| author | Frederika Chovancová Marjan Motiei Ivana Šišoláková Michal Urbánek Jana Shepa Haojie Fei Petr Sáha Renáta Oriňaková |
| author_facet | Frederika Chovancová Marjan Motiei Ivana Šišoláková Michal Urbánek Jana Shepa Haojie Fei Petr Sáha Renáta Oriňaková |
| author_sort | Frederika Chovancová |
| collection | DOAJ |
| description | Nickel modified chitosan nanoparticles are promising catalysts for the determination of bioanalytes such as insulin, glucose, antibiotics, and ascorbic acid. In this study, we synthesized nickel-loaded chitosan nanoparticles to evaluate their potential as surface modifiers for electrochemical sensors for insulin detection. The nanoparticles were prepared using the ionic gelation of chitosan with tripolyphosphate anions, followed by adsorption of nickel ions via ion-exchange resins and surface chelation. The physicochemical properties of the nanoparticles were characterized by scanning electron microscopy with EDX analysis, transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The catalytic activity of nickel modified chitosan nanoparticles towards insulin oxidation was investigated through cyclic voltammetry. The resulting screen-printed carbon electrode modified with nickel-chitosan nanoparticles exhibited exceptional analytical performance, including high sensitivity (0.09 mA μM), a low detection limit (0.02 μM), and a wide dynamic range (300 nM–5 μM). Additionally, the modified screen-printed electrode demonstrated excellent selectivity, enabling accurate insulin determination in the presence of interferences and in real blood serum samples. These findings highlight the potential of nickel-modified chitosan nanoparticles as a surface modification strategy to enhance the performance of electrochemical sensors insulin detection and pave the way for their application in various bioanalytes determination platforms. |
| format | Article |
| id | doaj-art-72ecfc0758d84cf991683e0d9993caf4 |
| institution | DOAJ |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-72ecfc0758d84cf991683e0d9993caf42025-08-20T02:57:02ZengElsevierBiosensors and Bioelectronics: X2590-13702025-09-012510062410.1016/j.biosx.2025.100624Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detectionFrederika Chovancová0Marjan Motiei1Ivana Šišoláková2Michal Urbánek3Jana Shepa4Haojie Fei5Petr Sáha6Renáta Oriňaková7Department of Physical Chemistry, Faculty of Science, Pavol Jozef Šafárik University, Moyzesova 11, 040 01, Košice, Slovak RepublicCentre of Polymer Systems, Tomáš Baťa University in Zlín, Třída Tomáše Bati 5678, 7601, Zlín, Czech RepublicDepartment of Physical Chemistry, Faculty of Science, Pavol Jozef Šafárik University, Moyzesova 11, 040 01, Košice, Slovak Republic; Corresponding author.Centre of Polymer Systems, Tomáš Baťa University in Zlín, Třída Tomáše Bati 5678, 7601, Zlín, Czech RepublicDepartment of Physical Chemistry, Faculty of Science, Pavol Jozef Šafárik University, Moyzesova 11, 040 01, Košice, Slovak RepublicCentre of Polymer Systems, Tomáš Baťa University in Zlín, Třída Tomáše Bati 5678, 7601, Zlín, Czech RepublicCentre of Polymer Systems, Tomáš Baťa University in Zlín, Třída Tomáše Bati 5678, 7601, Zlín, Czech RepublicDepartment of Physical Chemistry, Faculty of Science, Pavol Jozef Šafárik University, Moyzesova 11, 040 01, Košice, Slovak Republic; Centre of Polymer Systems, Tomáš Baťa University in Zlín, Třída Tomáše Bati 5678, 7601, Zlín, Czech RepublicNickel modified chitosan nanoparticles are promising catalysts for the determination of bioanalytes such as insulin, glucose, antibiotics, and ascorbic acid. In this study, we synthesized nickel-loaded chitosan nanoparticles to evaluate their potential as surface modifiers for electrochemical sensors for insulin detection. The nanoparticles were prepared using the ionic gelation of chitosan with tripolyphosphate anions, followed by adsorption of nickel ions via ion-exchange resins and surface chelation. The physicochemical properties of the nanoparticles were characterized by scanning electron microscopy with EDX analysis, transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The catalytic activity of nickel modified chitosan nanoparticles towards insulin oxidation was investigated through cyclic voltammetry. The resulting screen-printed carbon electrode modified with nickel-chitosan nanoparticles exhibited exceptional analytical performance, including high sensitivity (0.09 mA μM), a low detection limit (0.02 μM), and a wide dynamic range (300 nM–5 μM). Additionally, the modified screen-printed electrode demonstrated excellent selectivity, enabling accurate insulin determination in the presence of interferences and in real blood serum samples. These findings highlight the potential of nickel-modified chitosan nanoparticles as a surface modification strategy to enhance the performance of electrochemical sensors insulin detection and pave the way for their application in various bioanalytes determination platforms.http://www.sciencedirect.com/science/article/pii/S2590137025000512NickelChitosan nanoparticlesInsulinSensorElectrochemistry |
| spellingShingle | Frederika Chovancová Marjan Motiei Ivana Šišoláková Michal Urbánek Jana Shepa Haojie Fei Petr Sáha Renáta Oriňaková Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection Biosensors and Bioelectronics: X Nickel Chitosan nanoparticles Insulin Sensor Electrochemistry |
| title | Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| title_full | Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| title_fullStr | Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| title_full_unstemmed | Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| title_short | Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| title_sort | synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection |
| topic | Nickel Chitosan nanoparticles Insulin Sensor Electrochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000512 |
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