Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i>
<b>Background/Objectives:</b> This study aimed to develop gel nanoemulsions (NEs) of Brazilian essential oils (EOs) from <i>Eugenia uniflora</i> and <i>Psidium guajava</i>, as well as to perform chemical characterization and investigate the antimicrobial activity...
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MDPI AG
2025-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/1/93 |
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| author | Rebeca Dias dos Santos Breno Noronha Matos Daniel Oliveira Freire Franklyn Santos da Silva Bruno Alcântara do Prado Karolina Oliveira Gomes Marta Oliveira de Araújo Carla Azevedo Bilac Letícia Fernandes Silva Rodrigues Izabel Cristina Rodrigues da Silva Lívia Cristina Lira de Sá Barreto Claudio Augusto Gomes da Camara Marcilio Martins de Moraes Guilherme Martins Gelfuso Daniela Castilho Orsi |
| author_facet | Rebeca Dias dos Santos Breno Noronha Matos Daniel Oliveira Freire Franklyn Santos da Silva Bruno Alcântara do Prado Karolina Oliveira Gomes Marta Oliveira de Araújo Carla Azevedo Bilac Letícia Fernandes Silva Rodrigues Izabel Cristina Rodrigues da Silva Lívia Cristina Lira de Sá Barreto Claudio Augusto Gomes da Camara Marcilio Martins de Moraes Guilherme Martins Gelfuso Daniela Castilho Orsi |
| author_sort | Rebeca Dias dos Santos |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> This study aimed to develop gel nanoemulsions (NEs) of Brazilian essential oils (EOs) from <i>Eugenia uniflora</i> and <i>Psidium guajava</i>, as well as to perform chemical characterization and investigate the antimicrobial activity of the EOs and NEs. <b>Results/Conclusions</b>: The main chemical compounds of <i>E. uniflora</i> EO were curzerene (34.80%) and germacrene B (11.92%), while those of <i>P. guajava</i> EO were β-caryophyllene (25.92%), β-selinene (22.64%), and γ-selinene (19.13%). The NEs of <i>E. uniflora</i> and <i>P. guajava</i> had droplet sizes of 105.30 and 99.50 nm and polydispersity index (PDI) values of 0.32 and 0.43, respectively. The NEs remained stable for 30 days of storage at 25 °C, with droplet sizes of 104.7 and 103.8 nm, PDI values below 0.50, and no phase separation. The NE of <i>E. uniflora</i> exhibited inhibition zones ranging from 8.41 to 15.13 mm against the Gram-positive bacterium <i>Staphylococcus aureus</i> and the Gram-negative bacteria <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Acinetobacter baumannii</i>. Additionally, the NE of <i>E. uniflora</i> showed the largest inhibition zones against <i>Candida albicans</i> (20.97 mm) and <i>Candida krusei</i> (15.20 mm), along with low minimum inhibitory concentration (MIC) values (0.54–1.22 mg/mL) and minimal bactericidal concentration (MBC) values (4.84–11.02 mg/mL) against these pathogenic yeasts. The NE of <i>P. guajava</i> demonstrated low MIC (1.26 mg/mL) and MBC (11.35 mg/mL) values for <i>C. krusei</i>. The time–growth inhibition assay also suggests the effectiveness of the NE against the tested pathogens <i>S. aureus</i> and <i>E. coli</i>, highlighting its potential as a novel alternative therapeutic agent. |
| format | Article |
| id | doaj-art-72eb285b7e05443a96e50893935e9a75 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-72eb285b7e05443a96e50893935e9a752025-01-24T13:18:57ZengMDPI AGAntibiotics2079-63822025-01-011419310.3390/antibiotics14010093Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i>Rebeca Dias dos Santos0Breno Noronha Matos1Daniel Oliveira Freire2Franklyn Santos da Silva3Bruno Alcântara do Prado4Karolina Oliveira Gomes5Marta Oliveira de Araújo6Carla Azevedo Bilac7Letícia Fernandes Silva Rodrigues8Izabel Cristina Rodrigues da Silva9Lívia Cristina Lira de Sá Barreto10Claudio Augusto Gomes da Camara11Marcilio Martins de Moraes12Guilherme Martins Gelfuso13Daniela Castilho Orsi14Laboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Food, Drugs, and Cosmetics, University of Brasília (UNB/FS), Brasília 70910-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, BrazilDepartment of Chemistry, Federal Rural University of Pernambuco, Recife 50740-560, PE, BrazilDepartment of Chemistry, Federal Rural University of Pernambuco, Recife 50740-560, PE, BrazilLaboratory of Food, Drugs, and Cosmetics, University of Brasília (UNB/FS), Brasília 70910-900, DF, BrazilLaboratory of Quality Control, University of Brasília (UNB/FCE), Centro Metropolitano, Conjunto A, Lote 01, Ceilândia, Brasília 72220-900, DF, Brazil<b>Background/Objectives:</b> This study aimed to develop gel nanoemulsions (NEs) of Brazilian essential oils (EOs) from <i>Eugenia uniflora</i> and <i>Psidium guajava</i>, as well as to perform chemical characterization and investigate the antimicrobial activity of the EOs and NEs. <b>Results/Conclusions</b>: The main chemical compounds of <i>E. uniflora</i> EO were curzerene (34.80%) and germacrene B (11.92%), while those of <i>P. guajava</i> EO were β-caryophyllene (25.92%), β-selinene (22.64%), and γ-selinene (19.13%). The NEs of <i>E. uniflora</i> and <i>P. guajava</i> had droplet sizes of 105.30 and 99.50 nm and polydispersity index (PDI) values of 0.32 and 0.43, respectively. The NEs remained stable for 30 days of storage at 25 °C, with droplet sizes of 104.7 and 103.8 nm, PDI values below 0.50, and no phase separation. The NE of <i>E. uniflora</i> exhibited inhibition zones ranging from 8.41 to 15.13 mm against the Gram-positive bacterium <i>Staphylococcus aureus</i> and the Gram-negative bacteria <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Acinetobacter baumannii</i>. Additionally, the NE of <i>E. uniflora</i> showed the largest inhibition zones against <i>Candida albicans</i> (20.97 mm) and <i>Candida krusei</i> (15.20 mm), along with low minimum inhibitory concentration (MIC) values (0.54–1.22 mg/mL) and minimal bactericidal concentration (MBC) values (4.84–11.02 mg/mL) against these pathogenic yeasts. The NE of <i>P. guajava</i> demonstrated low MIC (1.26 mg/mL) and MBC (11.35 mg/mL) values for <i>C. krusei</i>. The time–growth inhibition assay also suggests the effectiveness of the NE against the tested pathogens <i>S. aureus</i> and <i>E. coli</i>, highlighting its potential as a novel alternative therapeutic agent.https://www.mdpi.com/2079-6382/14/1/93pitanga essential oilguajava essential oilnanoemulsionantifungal activityantibacterial activity |
| spellingShingle | Rebeca Dias dos Santos Breno Noronha Matos Daniel Oliveira Freire Franklyn Santos da Silva Bruno Alcântara do Prado Karolina Oliveira Gomes Marta Oliveira de Araújo Carla Azevedo Bilac Letícia Fernandes Silva Rodrigues Izabel Cristina Rodrigues da Silva Lívia Cristina Lira de Sá Barreto Claudio Augusto Gomes da Camara Marcilio Martins de Moraes Guilherme Martins Gelfuso Daniela Castilho Orsi Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> Antibiotics pitanga essential oil guajava essential oil nanoemulsion antifungal activity antibacterial activity |
| title | Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> |
| title_full | Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> |
| title_fullStr | Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> |
| title_full_unstemmed | Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> |
| title_short | Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of <i>Eugenia uniflora</i> and <i>Psidium guajava</i> |
| title_sort | chemical characterization and antimicrobial activity of essential oils and nanoemulsions of i eugenia uniflora i and i psidium guajava i |
| topic | pitanga essential oil guajava essential oil nanoemulsion antifungal activity antibacterial activity |
| url | https://www.mdpi.com/2079-6382/14/1/93 |
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