Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron

The pot experiments were applied to study the effect of nitrogen (N) deficiency on the gas exchange, chlorophyll fluorescence, photosynthetic pigment contents, chloroplast ultrastructure and the activities of antioxidative enzymes in fingered citron (Citrus medica var. sarcodactylis Swingle). The re...

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Main Authors: GUO Wei-dong, SANG Dan, ZHENG Jian-shu, LIAO Hai-bing, CHEN Wen-rong
Format: Article
Language:English
Published: Zhejiang University Press 2009-05-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.012
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author GUO Wei-dong
SANG Dan
ZHENG Jian-shu
LIAO Hai-bing
CHEN Wen-rong
author_facet GUO Wei-dong
SANG Dan
ZHENG Jian-shu
LIAO Hai-bing
CHEN Wen-rong
author_sort GUO Wei-dong
collection DOAJ
description The pot experiments were applied to study the effect of nitrogen (N) deficiency on the gas exchange, chlorophyll fluorescence, photosynthetic pigment contents, chloroplast ultrastructure and the activities of antioxidative enzymes in fingered citron (Citrus medica var. sarcodactylis Swingle). The results show that N deficiency caused a significant reduction in chlorophyll (Chl), chlorophyll a (Chla) content and the ratio of Chla/chlorophyll b (Chlb), and increased the ratio of carotenoids (Car) / Chl. Under the treatment of N deficiency, the gas exchange rate, the maximum efficiency of PSII photochemistry (Fv/Fm), the effective PS II quantum yield (Φ PS Ⅱ), the photochemical quenching (qP) and the electron transport rate (ETR) were significantly declined. Meanwhile, the minimum fluorescence yield (Fo) and the nonphotochemical quenching (qN) increased with N deficiency. We also found the declines in the activities of superoxide dismutase isoenzyme (SOD), peroxidase (POD) and catalase (CAT), the increase of malondialdehyde (MDA) contents in the stressed leaves and the disordered chloroplast lamellae structure. It was indicated that N deficiency reduced primarily the photochemical pigment contents, decreased the electron transport efficiency, then induced the lipid peroxidation, which resulted in the injuries of chloroplast lamellae structure and the photosynthetic centers, and finally, induced the decline in the net photosynthetic rate.
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spelling doaj-art-72e4ff05d00a42ff879f2b9bbcc2c8b42025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-05-013530731410.3785/j.issn.1008-9209.2009.03.01210089209Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citronGUO Wei-dongSANG DanZHENG Jian-shuLIAO Hai-bingCHEN Wen-rongThe pot experiments were applied to study the effect of nitrogen (N) deficiency on the gas exchange, chlorophyll fluorescence, photosynthetic pigment contents, chloroplast ultrastructure and the activities of antioxidative enzymes in fingered citron (Citrus medica var. sarcodactylis Swingle). The results show that N deficiency caused a significant reduction in chlorophyll (Chl), chlorophyll a (Chla) content and the ratio of Chla/chlorophyll b (Chlb), and increased the ratio of carotenoids (Car) / Chl. Under the treatment of N deficiency, the gas exchange rate, the maximum efficiency of PSII photochemistry (Fv/Fm), the effective PS II quantum yield (Φ PS Ⅱ), the photochemical quenching (qP) and the electron transport rate (ETR) were significantly declined. Meanwhile, the minimum fluorescence yield (Fo) and the nonphotochemical quenching (qN) increased with N deficiency. We also found the declines in the activities of superoxide dismutase isoenzyme (SOD), peroxidase (POD) and catalase (CAT), the increase of malondialdehyde (MDA) contents in the stressed leaves and the disordered chloroplast lamellae structure. It was indicated that N deficiency reduced primarily the photochemical pigment contents, decreased the electron transport efficiency, then induced the lipid peroxidation, which resulted in the injuries of chloroplast lamellae structure and the photosynthetic centers, and finally, induced the decline in the net photosynthetic rate.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.012fingered citronnitrogen deficiencyphotosynthesischlorophyll fluorescencechloroplast ultrastructure
spellingShingle GUO Wei-dong
SANG Dan
ZHENG Jian-shu
LIAO Hai-bing
CHEN Wen-rong
Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
浙江大学学报. 农业与生命科学版
fingered citron
nitrogen deficiency
photosynthesis
chlorophyll fluorescence
chloroplast ultrastructure
title Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
title_full Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
title_fullStr Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
title_full_unstemmed Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
title_short Effects of nitrogen deficiency on the gas exchange, chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
title_sort effects of nitrogen deficiency on the gas exchange chlorophyll fluorescence and chloroplast ultrastructure in fingered citron
topic fingered citron
nitrogen deficiency
photosynthesis
chlorophyll fluorescence
chloroplast ultrastructure
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.012
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