Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection
The study aimed to evaluate the effect of a trypsin inhibitor isolated from tamarind seeds (TTI) on Caenorhabditis elegans as an anti-infective agent. No statistical difference was found in the toxicity test to TTI for locomotion, oviposition, and progeny parameters. Regarding body size, the tested...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14756366.2025.2484400 |
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| author | Lídia Leonize Rodrigues Matias Riva de Paula Oliveira Rafael Oliveira de Araújo Costa Wendjilla Fortunato de Medeiros Ana Francisca Teixeira Gomes Hugo Alexandre Oliveira Rocha Karla Suzane Florentino da Silva Chaves Damasceno Thaís Souza Passos Ana Heloneida de Araujo Morais |
| author_facet | Lídia Leonize Rodrigues Matias Riva de Paula Oliveira Rafael Oliveira de Araújo Costa Wendjilla Fortunato de Medeiros Ana Francisca Teixeira Gomes Hugo Alexandre Oliveira Rocha Karla Suzane Florentino da Silva Chaves Damasceno Thaís Souza Passos Ana Heloneida de Araujo Morais |
| author_sort | Lídia Leonize Rodrigues Matias |
| collection | DOAJ |
| description | The study aimed to evaluate the effect of a trypsin inhibitor isolated from tamarind seeds (TTI) on Caenorhabditis elegans as an anti-infective agent. No statistical difference was found in the toxicity test to TTI for locomotion, oviposition, and progeny parameters. Regarding body size, the tested concentrations of TTI showed a statistical difference and resulted in a greater length of C. elegans than the control. For bacterial infection analyses, 0.1 mg/mL and 1.0 mg/mL of TTI promoted a survival rate of 16.10% and 30.32%, respectively, higher than the control (8.08%). For the oxidative stress test, all concentrations of TTI presented greater survival than the control at 12h and 18h (p < 0.05). Regarding the bacterial growth curve, no inhibitory activity of TTI was observed in E. coli (OP50), nor was antibacterial and antioxidant activity in vitro. Therefore, given all the tests, TTI protected C. elegans under adverse conditions, increasing their survival. |
| format | Article |
| id | doaj-art-72df082ec2594dbbba6091f475090d2e |
| institution | OA Journals |
| issn | 1475-6366 1475-6374 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj-art-72df082ec2594dbbba6091f475090d2e2025-08-20T02:16:23ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742025-12-0140110.1080/14756366.2025.2484400Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infectionLídia Leonize Rodrigues Matias0Riva de Paula Oliveira1Rafael Oliveira de Araújo Costa2Wendjilla Fortunato de Medeiros3Ana Francisca Teixeira Gomes4Hugo Alexandre Oliveira Rocha5Karla Suzane Florentino da Silva Chaves Damasceno6Thaís Souza Passos7Ana Heloneida de Araujo Morais8Postgraduate Program in Biochemistry and Molecular Biology, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, BrazilDepartment of Cell Biology and Genetics Center of Biosciences, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Biochemistry and Molecular Biology, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Biochemistry and Molecular Biology, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, BrazilPostgraduate Program in Biochemistry and Molecular Biology, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, BrazilThe study aimed to evaluate the effect of a trypsin inhibitor isolated from tamarind seeds (TTI) on Caenorhabditis elegans as an anti-infective agent. No statistical difference was found in the toxicity test to TTI for locomotion, oviposition, and progeny parameters. Regarding body size, the tested concentrations of TTI showed a statistical difference and resulted in a greater length of C. elegans than the control. For bacterial infection analyses, 0.1 mg/mL and 1.0 mg/mL of TTI promoted a survival rate of 16.10% and 30.32%, respectively, higher than the control (8.08%). For the oxidative stress test, all concentrations of TTI presented greater survival than the control at 12h and 18h (p < 0.05). Regarding the bacterial growth curve, no inhibitory activity of TTI was observed in E. coli (OP50), nor was antibacterial and antioxidant activity in vitro. Therefore, given all the tests, TTI protected C. elegans under adverse conditions, increasing their survival.https://www.tandfonline.com/doi/10.1080/14756366.2025.2484400Agents antibacterialsinfectionsTamarindus indica L |
| spellingShingle | Lídia Leonize Rodrigues Matias Riva de Paula Oliveira Rafael Oliveira de Araújo Costa Wendjilla Fortunato de Medeiros Ana Francisca Teixeira Gomes Hugo Alexandre Oliveira Rocha Karla Suzane Florentino da Silva Chaves Damasceno Thaís Souza Passos Ana Heloneida de Araujo Morais Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection Journal of Enzyme Inhibition and Medicinal Chemistry Agents antibacterials infections Tamarindus indica L |
| title | Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection |
| title_full | Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection |
| title_fullStr | Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection |
| title_full_unstemmed | Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection |
| title_short | Effect of trypsin inhibitor isolated from tamarind seed (TTI) on caenorhabditis elegans survival under bacterial infection |
| title_sort | effect of trypsin inhibitor isolated from tamarind seed tti on caenorhabditis elegans survival under bacterial infection |
| topic | Agents antibacterials infections Tamarindus indica L |
| url | https://www.tandfonline.com/doi/10.1080/14756366.2025.2484400 |
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