An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction
Abstract Sensory-selective nerve blockade is highly useful for pain management in clinical practice, but developing such blockers remains challenging. A major handicap is the lack of objective in vivo animal models to evaluate motor and sensory nerve conduction simultaneously. Due to anatomical stru...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-12201-5 |
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| author | Yi Teng Xian Zou Jin Liu Xiangyu Hu Cheng Zhou Mengchan Ou |
| author_facet | Yi Teng Xian Zou Jin Liu Xiangyu Hu Cheng Zhou Mengchan Ou |
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| description | Abstract Sensory-selective nerve blockade is highly useful for pain management in clinical practice, but developing such blockers remains challenging. A major handicap is the lack of objective in vivo animal models to evaluate motor and sensory nerve conduction simultaneously. Due to anatomical structures, motor evoked potentials (MEPs) and/or somatosensory evoked potentials (SSEPs) may be used to assess nerve conduction. MEPs were elicited by stimulating motor cortex and recorded from contralateral hind limb. SSEPs were generated by stimulating sciatic nerve and recorded from contralateral sensory cortex. Amplitude/latency changes were recorded under various physiological conditions (e.g., anesthesia, durations, temperatures, and oxygen) and drug interventions for validation. Compared to sevoflurane, propofol minimally inhibited MEPs at sedative doses and was therefore used during recordings. Both hypothermia (34–36 °C) and hyperthermia (38–40 °C) significantly suppressed MEP and SSEP amplitudes (P < 0.0001). Reduced oxygen saturation (SaO2) decreased MEP amplitudes (P < 0.0001), and the amplitudes were strongly correlated with SaO2 (R2 = 0.8284). For selective blockade validation, lidocaine suppressed both MEP and SSEP amplitudes below 20% of baseline (P < 0.0001), confirming non-selectivity. In contrast, QX-314/capsaicin selectively suppressed SSEPs (P < 0.0001), while MEPs remained stable. This model is stable for evaluating selective nerve blockade in vivo for at least 60 min under controlled physiological conditions. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-72dc553dad08490abda1b1c2dbeb191c2025-08-20T04:02:46ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-12201-5An in vivo rat model for comparing selective blockade between sensory and motor nerve conductionYi Teng0Xian Zou1Jin Liu2Xiangyu Hu3Cheng Zhou4Mengchan Ou5Department of Anesthesiology, West China Hospital of Sichuan UniversityDepartment of Anesthesiology, West China Hospital of Sichuan UniversityDepartment of Anesthesiology, West China Hospital of Sichuan UniversityDepartment of Anesthesiology, West China Hospital of Sichuan UniversityResearch Center of Anesthesiology, West China Hospital, Sichuan UniversityDepartment of Anesthesiology, West China Hospital of Sichuan UniversityAbstract Sensory-selective nerve blockade is highly useful for pain management in clinical practice, but developing such blockers remains challenging. A major handicap is the lack of objective in vivo animal models to evaluate motor and sensory nerve conduction simultaneously. Due to anatomical structures, motor evoked potentials (MEPs) and/or somatosensory evoked potentials (SSEPs) may be used to assess nerve conduction. MEPs were elicited by stimulating motor cortex and recorded from contralateral hind limb. SSEPs were generated by stimulating sciatic nerve and recorded from contralateral sensory cortex. Amplitude/latency changes were recorded under various physiological conditions (e.g., anesthesia, durations, temperatures, and oxygen) and drug interventions for validation. Compared to sevoflurane, propofol minimally inhibited MEPs at sedative doses and was therefore used during recordings. Both hypothermia (34–36 °C) and hyperthermia (38–40 °C) significantly suppressed MEP and SSEP amplitudes (P < 0.0001). Reduced oxygen saturation (SaO2) decreased MEP amplitudes (P < 0.0001), and the amplitudes were strongly correlated with SaO2 (R2 = 0.8284). For selective blockade validation, lidocaine suppressed both MEP and SSEP amplitudes below 20% of baseline (P < 0.0001), confirming non-selectivity. In contrast, QX-314/capsaicin selectively suppressed SSEPs (P < 0.0001), while MEPs remained stable. This model is stable for evaluating selective nerve blockade in vivo for at least 60 min under controlled physiological conditions.https://doi.org/10.1038/s41598-025-12201-5Selective nerve blockadeEvoked potentialsSensory-motor separationLocal anestheticsIn vivo animal model |
| spellingShingle | Yi Teng Xian Zou Jin Liu Xiangyu Hu Cheng Zhou Mengchan Ou An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction Scientific Reports Selective nerve blockade Evoked potentials Sensory-motor separation Local anesthetics In vivo animal model |
| title | An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| title_full | An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| title_fullStr | An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| title_full_unstemmed | An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| title_short | An in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| title_sort | in vivo rat model for comparing selective blockade between sensory and motor nerve conduction |
| topic | Selective nerve blockade Evoked potentials Sensory-motor separation Local anesthetics In vivo animal model |
| url | https://doi.org/10.1038/s41598-025-12201-5 |
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