MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic
Cholera remains a topical infection in the world, which determines the high risk of importation of the pathogen on the territory of the Russian Federation. MLVA-typing of 135 strains at 5 tandem repeats loci was carried outin order to study the clonal-population structure of the isolated from 1973 t...
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| Format: | Article |
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Numikom LLC
2017-06-01
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| Series: | Эпидемиология и вакцинопрофилактика |
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| Online Access: | https://www.epidemvac.ru/jour/article/view/276 |
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| author | A. S. Ponomareva L. V. Mironova Zh. Yu. Khunkheeva A. B. Moshkin S. V. Balakhonov |
| author_facet | A. S. Ponomareva L. V. Mironova Zh. Yu. Khunkheeva A. B. Moshkin S. V. Balakhonov |
| author_sort | A. S. Ponomareva |
| collection | DOAJ |
| description | Cholera remains a topical infection in the world, which determines the high risk of importation of the pathogen on the territory of the Russian Federation. MLVA-typing of 135 strains at 5 tandem repeats loci was carried outin order to study the clonal-population structure of the isolated from 1973 to 2016 on the Transbaikal territory V. cholerae. Analysis of the distribution patterns showed that the primary emergence of strains with new atypical for the territory genotypes in 1970s, 1980s, 1990s, occurred in sewage waters with the subsequent detection of the given genotypes generally in Ingoda river and Kenon lake. The strains firstly isolated from Borzya river and Haranor lake and found out at various times in Ingoda river and Kenon lake, Chita, Argun rivers, Haranor lake and Borzya river have been dominating since 2005 in Transbaikal territory. Finding groups of isolates with identical or similar MLVA-profile appeared in cluster complexes evidence of their long-term preservation in specific ecological niches, strains’ allelic profiles undergo minor transformation on the most variable loci in the process of adaptation. Identification of V. cholerae El Tor that variable tandem repeat loci structure materially differs from those of the dominant clones may serve as proof of their invasive origin. |
| format | Article |
| id | doaj-art-72da494f3187419eafedd9e9a5d8367a |
| institution | Kabale University |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| publishDate | 2017-06-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj-art-72da494f3187419eafedd9e9a5d8367a2025-08-20T03:43:46ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942017-06-01163505710.31631/2073-3046-2017-16-3-50-57275MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh PandemicA. S. Ponomareva0L. V. Mironova1Zh. Yu. Khunkheeva2A. B. Moshkin3S. V. Balakhonov4Federal Budgetary Healthcare Facility «Irkutsk Antiplague Research Institute» of Federal Service of Surveillance on Consumer, Rights Protection and Human WellbeingFederal Budgetary Healthcare Facility «Irkutsk Antiplague Research Institute» of Federal Service of Surveillance on Consumer, Rights Protection and Human WellbeingFederal Budgetary Healthcare Facility «Irkutsk Antiplague Research Institute» of Federal Service of Surveillance on Consumer, Rights Protection and Human WellbeingFederal Budgetary Healthcare Facility «Chita Antiplague Station» of Federal Service of Surveillance on Consumer, Rights Protection and Human WellbeingFederal Budgetary Healthcare Facility «Irkutsk Antiplague Research Institute» of Federal Service of Surveillance on Consumer, Rights Protection and Human WellbeingCholera remains a topical infection in the world, which determines the high risk of importation of the pathogen on the territory of the Russian Federation. MLVA-typing of 135 strains at 5 tandem repeats loci was carried outin order to study the clonal-population structure of the isolated from 1973 to 2016 on the Transbaikal territory V. cholerae. Analysis of the distribution patterns showed that the primary emergence of strains with new atypical for the territory genotypes in 1970s, 1980s, 1990s, occurred in sewage waters with the subsequent detection of the given genotypes generally in Ingoda river and Kenon lake. The strains firstly isolated from Borzya river and Haranor lake and found out at various times in Ingoda river and Kenon lake, Chita, Argun rivers, Haranor lake and Borzya river have been dominating since 2005 in Transbaikal territory. Finding groups of isolates with identical or similar MLVA-profile appeared in cluster complexes evidence of their long-term preservation in specific ecological niches, strains’ allelic profiles undergo minor transformation on the most variable loci in the process of adaptation. Identification of V. cholerae El Tor that variable tandem repeat loci structure materially differs from those of the dominant clones may serve as proof of their invasive origin.https://www.epidemvac.ru/jour/article/view/276vibrio choleraeэпидемиологическое благополучиеаллельный профильпопуляционная структураmlva-типированиеvibrio choleraeepidemiological well-beingallelic profilepopulation structuremlva-typing |
| spellingShingle | A. S. Ponomareva L. V. Mironova Zh. Yu. Khunkheeva A. B. Moshkin S. V. Balakhonov MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic Эпидемиология и вакцинопрофилактика vibrio cholerae эпидемиологическое благополучие аллельный профиль популяционная структура mlva-типирование vibrio cholerae epidemiological well-being allelic profile population structure mlva-typing |
| title | MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic |
| title_full | MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic |
| title_fullStr | MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic |
| title_full_unstemmed | MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic |
| title_short | MLVA-typing of Vibrio choleraе El Tor Strains Isolated in Transbaikal Territory during the Seventh Pandemic |
| title_sort | mlva typing of vibrio choleraе el tor strains isolated in transbaikal territory during the seventh pandemic |
| topic | vibrio cholerae эпидемиологическое благополучие аллельный профиль популяционная структура mlva-типирование vibrio cholerae epidemiological well-being allelic profile population structure mlva-typing |
| url | https://www.epidemvac.ru/jour/article/view/276 |
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