Molecular identification of Potyvirus infecting Lilium cvs. (Asiatic hybrids) in Lishui, Zhejiang
Two isolates, G and X were gained from Lilium cvs. (Asiatic hybrids) in Lishui of Zhejiang Province. Numerous filamentous virus particles resembling Potyvirus were detected by electron microscopy. Pinwheel inclusions were observed in the cytoplasm of parenchyma cells. Based on a primer specially des...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2005-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.02.0185 |
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| Summary: | Two isolates, G and X were gained from Lilium cvs. (Asiatic hybrids) in Lishui of Zhejiang Province. Numerous filamentous virus particles resembling Potyvirus were detected by electron microscopy. Pinwheel inclusions were observed in the cytoplasm of parenchyma cells. Based on a primer specially designed for the genome sequences by the member of Potyvirus and Lily mottle virus (LMoV), two partial sequences of cDNA were cloned by RT-PCR. They were composed of 1692 nt and 1469 nt respectively, both including NIb, CP, 3′-URT, and poly (A) of 3′ end. Similarity of CP sequences between G and X isolates were compared with ten isolates of Potyvirus infecting lily and tulip plants from other region of the world. The result showed that G and X have high homology with LMoV in CP nucleotide sequence and CP amino acid sequence. They varied from 85.9% to 99.4%, 86.1% to 99.2% and from 87.0% to 96.7%, 89.9% to 97.8% respectively, and the two isolates was thus confirmed as LMoV. The phylogenetic tree topology analysis suggested G and X be divided into two different groups. They have low homology and their evolution were not related with the host and geological origination. |
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| ISSN: | 1008-9209 2097-5155 |