Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma
Aim. To evaluate the changes in the total cellularity (Nuc) of the rat sciatic nerve regenerative neuroma (RegN) and its representation of CD90, CD44, CD146, and CD133 positive cells, as well as its neurotization under the conditions of dexamethasone (Dex) and granulocyte colony-stimulating factor (...
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Zaporizhzhia State Medical and Pharmaceutical University
2023-12-01
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| Series: | Patologìâ |
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| Online Access: | http://pat.zsmu.edu.ua/article/view/288552/288274 |
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| author | O. M. Grabovyi N. M. Nevmerzhytska O. B. Alyokhin H. B. Kostynskyi H. Yu. Kondaurova |
| author_facet | O. M. Grabovyi N. M. Nevmerzhytska O. B. Alyokhin H. B. Kostynskyi H. Yu. Kondaurova |
| author_sort | O. M. Grabovyi |
| collection | DOAJ |
| description | Aim. To evaluate the changes in the total cellularity (Nuc) of the rat sciatic nerve regenerative neuroma (RegN) and its representation of CD90, CD44, CD146, and CD133 positive cells, as well as its neurotization under the conditions of dexamethasone (Dex) and granulocyte colony-stimulating factor (GCSF).
Materials and methods. After injections of dexamethasone and granulocyte colony-stimulating factor in the regenerative neuroma of 168 male Wistar rats, CD90, CD44, CD146 and CD133 positive cells were detected immunohistochemically in the regenerative neuroma. Its total cellularity and neurotization were determined and statistical processing of the obtained data was performed.
Results. It was found that dexamethasone and granulocyte colony-stimulating factor change the representation of cellular patterns and neurotization of regenerative neuroma.
Conclusions. The amount of neurotization of the regeneration neuroma directly depends on the specific amount of Schwann cells in its composition. Dex and GCSF, despite different mechanisms of action, lead to an increase in the content of neuroleumocytes in RegN. |
| format | Article |
| id | doaj-art-72d0f4d5e4844e90aa12fb0ebd78b06a |
| institution | DOAJ |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Patologìâ |
| spelling | doaj-art-72d0f4d5e4844e90aa12fb0ebd78b06a2025-08-20T03:19:03ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372023-12-0120322223010.14739/2310-1237.2023.3.288552Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuromaO. M. Grabovyi0https://orcid.org/0000-0001-5705-9909N. M. Nevmerzhytska1https://orcid.org/0000-0002-5378-2267O. B. Alyokhin2https://orcid.org/0000-0001-5209-8036H. B. Kostynskyi3https://orcid.org/0000-0002-4984-9764H. Yu. Kondaurova4Bogomolets National Medical University, Kyiv, UkraineBogomolets National Medical University, Kyiv, UkraineMariupol State University, Kyiv, UkrainePrivate Higher Education Institution “International European University”, Kyiv, UkraineBogomolets National Medical University, Kyiv, UkraineAim. To evaluate the changes in the total cellularity (Nuc) of the rat sciatic nerve regenerative neuroma (RegN) and its representation of CD90, CD44, CD146, and CD133 positive cells, as well as its neurotization under the conditions of dexamethasone (Dex) and granulocyte colony-stimulating factor (GCSF). Materials and methods. After injections of dexamethasone and granulocyte colony-stimulating factor in the regenerative neuroma of 168 male Wistar rats, CD90, CD44, CD146 and CD133 positive cells were detected immunohistochemically in the regenerative neuroma. Its total cellularity and neurotization were determined and statistical processing of the obtained data was performed. Results. It was found that dexamethasone and granulocyte colony-stimulating factor change the representation of cellular patterns and neurotization of regenerative neuroma. Conclusions. The amount of neurotization of the regeneration neuroma directly depends on the specific amount of Schwann cells in its composition. Dex and GCSF, despite different mechanisms of action, lead to an increase in the content of neuroleumocytes in RegN.http://pat.zsmu.edu.ua/article/view/288552/288274nerve regenerationdexamethasonegranulocyte colony-stimulating factor |
| spellingShingle | O. M. Grabovyi N. M. Nevmerzhytska O. B. Alyokhin H. B. Kostynskyi H. Yu. Kondaurova Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma Patologìâ nerve regeneration dexamethasone granulocyte colony-stimulating factor |
| title | Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| title_full | Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| title_fullStr | Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| title_full_unstemmed | Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| title_short | Dexamethasone and granulocyte colony-stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| title_sort | dexamethasone and granulocyte colony stimulating factor alter the representation of cellular patterns and neurotisation of regenerative neuroma |
| topic | nerve regeneration dexamethasone granulocyte colony-stimulating factor |
| url | http://pat.zsmu.edu.ua/article/view/288552/288274 |
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