Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease
Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD,...
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/450957 |
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| author | Daniel Kretzschmar Ilonka Rohm Sebastian Schäller Stefan Betge Rudin Pistulli Yevgeniya Atiskova Hans-R. Figulla Atilla Yilmaz |
| author_facet | Daniel Kretzschmar Ilonka Rohm Sebastian Schäller Stefan Betge Rudin Pistulli Yevgeniya Atiskova Hans-R. Figulla Atilla Yilmaz |
| author_sort | Daniel Kretzschmar |
| collection | DOAJ |
| description | Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n=52) compared to controls (n=60). Femoralis plaques (n=12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm2, P=0.02; CD83: 19 versus 5 cells/0.1 mm2, P=0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques. |
| format | Article |
| id | doaj-art-72c3b5826d2e46aa8494e7a1028990dd |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-72c3b5826d2e46aa8494e7a1028990dd2025-02-03T06:44:38ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/450957450957Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery DiseaseDaniel Kretzschmar0Ilonka Rohm1Sebastian Schäller2Stefan Betge3Rudin Pistulli4Yevgeniya Atiskova5Hans-R. Figulla6Atilla Yilmaz7Department of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyDepartment of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, GermanyPeripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n=52) compared to controls (n=60). Femoralis plaques (n=12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm2, P=0.02; CD83: 19 versus 5 cells/0.1 mm2, P=0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.http://dx.doi.org/10.1155/2015/450957 |
| spellingShingle | Daniel Kretzschmar Ilonka Rohm Sebastian Schäller Stefan Betge Rudin Pistulli Yevgeniya Atiskova Hans-R. Figulla Atilla Yilmaz Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease Mediators of Inflammation |
| title | Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease |
| title_full | Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease |
| title_fullStr | Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease |
| title_full_unstemmed | Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease |
| title_short | Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease |
| title_sort | decrease in circulating dendritic cell precursors in patients with peripheral artery disease |
| url | http://dx.doi.org/10.1155/2015/450957 |
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