Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma
Universally Primed PCR (UP-PCR) is a PCR fingerprinting method that has demonstrated its applicability in different aspects of mycology. These applications constitute analysis of genome structures, identification of species, analysis of population and species diversity, revealing of genetic relatedn...
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Zhejiang University Press
2004-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.04.0463 |
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| author | Mette Lübeck |
| author_facet | Mette Lübeck |
| author_sort | Mette Lübeck |
| collection | DOAJ |
| description | Universally Primed PCR (UP-PCR) is a PCR fingerprinting method that has demonstrated its applicability in different aspects of mycology. These applications constitute analysis of genome structures, identification of species, analysis of population and species diversity, revealing of genetic relatedness at infra-and inter-species level, and identification of UP-PCR markers at different taxonomic levels (strain, group and/or species). A further development of the UP-PCR technique is an UP-PCR product cross hybridisation assay that facilitates investigation of sequence similarity (homology) of UP-PCR products and grouping of strains into UP-PCR hybridisation groups. This separates the strains into entities with high genetic similarity (DNA homology). UP-PCR has been used as an aid in taxonomy and species delineation, and to monitor biocontrol strains following their release into the environment by fingerprint characterisation of pure cultures and through direct detection in soil by amplification of UP-PCR-derived SCAR markers. The technique has been applied to Trichoderma strains in particularly with the aims of strain recognition and classification. |
| format | Article |
| id | doaj-art-72bfbdb8b71f445c83369463720e70eb |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-72bfbdb8b71f445c83369463720e70eb2025-08-20T02:52:51ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-07-013046346310.3785/1008-9209.2004.04.046310089209Universally Primed PCR (UP-PCR) and its applications for taxonomy in TrichodermaMette LübeckUniversally Primed PCR (UP-PCR) is a PCR fingerprinting method that has demonstrated its applicability in different aspects of mycology. These applications constitute analysis of genome structures, identification of species, analysis of population and species diversity, revealing of genetic relatedness at infra-and inter-species level, and identification of UP-PCR markers at different taxonomic levels (strain, group and/or species). A further development of the UP-PCR technique is an UP-PCR product cross hybridisation assay that facilitates investigation of sequence similarity (homology) of UP-PCR products and grouping of strains into UP-PCR hybridisation groups. This separates the strains into entities with high genetic similarity (DNA homology). UP-PCR has been used as an aid in taxonomy and species delineation, and to monitor biocontrol strains following their release into the environment by fingerprint characterisation of pure cultures and through direct detection in soil by amplification of UP-PCR-derived SCAR markers. The technique has been applied to Trichoderma strains in particularly with the aims of strain recognition and classification.https://www.academax.com/doi/10.3785/1008-9209.2004.04.0463UP-PCRfingerprintingstrain monitoringSCAR markers |
| spellingShingle | Mette Lübeck Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma 浙江大学学报. 农业与生命科学版 UP-PCR fingerprinting strain monitoring SCAR markers |
| title | Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma |
| title_full | Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma |
| title_fullStr | Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma |
| title_full_unstemmed | Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma |
| title_short | Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma |
| title_sort | universally primed pcr up pcr and its applications for taxonomy in trichoderma |
| topic | UP-PCR fingerprinting strain monitoring SCAR markers |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.04.0463 |
| work_keys_str_mv | AT mettelubeck universallyprimedpcruppcranditsapplicationsfortaxonomyintrichoderma |