Matcha alleviates obesity by modulating gut microbiota and its metabolites

Matcha shows promise for diabetes, obesity, and gut microbiota disorders. Studies suggest a significant link between gut microbiota, metabolites, and obesity. Thus, matcha may have a positive impact on obesity by modulating gut microbiota and metabolites. This study used 16S rDNA sequencing and unta...

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Main Authors: Yadan Luo, Yuhang Wen, Jingrong Huang, Baoting Chen, Shuya Lv, Hao Qiu, Shuaibing Li, Songwei Liu, Qian Yang, Lvqin He, Zehui Yu, Mingde Zhao, Manli He, Dong Li, Congwei Gu
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927124001497
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author Yadan Luo
Yuhang Wen
Jingrong Huang
Baoting Chen
Shuya Lv
Hao Qiu
Shuaibing Li
Songwei Liu
Qian Yang
Lvqin He
Zehui Yu
Mingde Zhao
Manli He
Dong Li
Congwei Gu
author_facet Yadan Luo
Yuhang Wen
Jingrong Huang
Baoting Chen
Shuya Lv
Hao Qiu
Shuaibing Li
Songwei Liu
Qian Yang
Lvqin He
Zehui Yu
Mingde Zhao
Manli He
Dong Li
Congwei Gu
author_sort Yadan Luo
collection DOAJ
description Matcha shows promise for diabetes, obesity, and gut microbiota disorders. Studies suggest a significant link between gut microbiota, metabolites, and obesity. Thus, matcha may have a positive impact on obesity by modulating gut microbiota and metabolites. This study used 16S rDNA sequencing and untargeted metabolomics to examine the cecal contents in mice. By correlation analysis, we explored the potential mechanisms responsible for the positive effects of matcha on obesity. The results indicated that matcha had a mitigating effect on the detrimental impacts of a high-fat diet (HFD) on multiple physiological indicators in mice, including body weight, adipose tissue weight, serum total cholesterol (TC), and low-density lipoprotein (LDL) levels, as well as glucose tolerance. Moreover, it was observed that matcha had an impact on the structural composition of gut microbiota and gut metabolites. Specifically, matcha was able to reverse the alterations in the abundance of certain obesity-improving bacteria, such as Alloprevotella, Ileibacterium, and Rikenella, as well as the abundance of obesity-promoting bacteria Romboutsia, induced by a HFD. Furthermore, matcha can influence the levels of metabolites, including formononetin, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate, within the gastrointestinal tract. Additionally, matcha enhances caffeine metabolism and the HIF-1 signaling pathway in the KEGG pathway. The results of the correlation analysis suggest that formononetin, theobromine, 1,3,7-trimethyluric acid, and Vitamin C displayed negative correlation with both the obesity phenotype and microbiota known to exacerbate obesity, while demonstrating positive correlations with microbiota that alleviated obesity. However, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate had the opposite effect. In conclusion, the impact of matcha on gut metabolites may be attributed to its modulation of the abundance of Alloprevotella, Ileibacterium, Rikenella, and Romboutsia within the gastrointestinal tract, thereby potentially contributing to the amelioration of obesity.
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spelling doaj-art-72bb7e5ce68a4e77836c15db287a8f582025-08-20T02:49:52ZengElsevierCurrent Research in Food Science2665-92712024-01-01910082310.1016/j.crfs.2024.100823Matcha alleviates obesity by modulating gut microbiota and its metabolitesYadan Luo0Yuhang Wen1Jingrong Huang2Baoting Chen3Shuya Lv4Hao Qiu5Shuaibing Li6Songwei Liu7Qian Yang8Lvqin He9Zehui Yu10Mingde Zhao11Manli He12Dong Li13Congwei Gu14Laboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang, Liaoning, 110016, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, China; Corresponding author. Laboratory Animal Centre, Southwest Medical University, Luzhou, China.College of Bioengineering, Sichuan University of Science and Engineering, Yibin, 643002, China; Corresponding author.Laboratory Animal Centre, Southwest Medical University, Luzhou, China; Model Animal and Human Disease Research of Luzhou Key Laboratory, Southwest Medical University, Luzhou, China; Department of Nutrition and Food Hygiene, School of Public Health, Southwest Medical University, Luzhou, China; Corresponding author. Laboratory Animal Centre, Southwest Medical University, Luzhou, China.Matcha shows promise for diabetes, obesity, and gut microbiota disorders. Studies suggest a significant link between gut microbiota, metabolites, and obesity. Thus, matcha may have a positive impact on obesity by modulating gut microbiota and metabolites. This study used 16S rDNA sequencing and untargeted metabolomics to examine the cecal contents in mice. By correlation analysis, we explored the potential mechanisms responsible for the positive effects of matcha on obesity. The results indicated that matcha had a mitigating effect on the detrimental impacts of a high-fat diet (HFD) on multiple physiological indicators in mice, including body weight, adipose tissue weight, serum total cholesterol (TC), and low-density lipoprotein (LDL) levels, as well as glucose tolerance. Moreover, it was observed that matcha had an impact on the structural composition of gut microbiota and gut metabolites. Specifically, matcha was able to reverse the alterations in the abundance of certain obesity-improving bacteria, such as Alloprevotella, Ileibacterium, and Rikenella, as well as the abundance of obesity-promoting bacteria Romboutsia, induced by a HFD. Furthermore, matcha can influence the levels of metabolites, including formononetin, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate, within the gastrointestinal tract. Additionally, matcha enhances caffeine metabolism and the HIF-1 signaling pathway in the KEGG pathway. The results of the correlation analysis suggest that formononetin, theobromine, 1,3,7-trimethyluric acid, and Vitamin C displayed negative correlation with both the obesity phenotype and microbiota known to exacerbate obesity, while demonstrating positive correlations with microbiota that alleviated obesity. However, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate had the opposite effect. In conclusion, the impact of matcha on gut metabolites may be attributed to its modulation of the abundance of Alloprevotella, Ileibacterium, Rikenella, and Romboutsia within the gastrointestinal tract, thereby potentially contributing to the amelioration of obesity.http://www.sciencedirect.com/science/article/pii/S2665927124001497MatchaObesityHigh-fat dietGut microbiotaUntargeted metabolomicsCorrelation analysis
spellingShingle Yadan Luo
Yuhang Wen
Jingrong Huang
Baoting Chen
Shuya Lv
Hao Qiu
Shuaibing Li
Songwei Liu
Qian Yang
Lvqin He
Zehui Yu
Mingde Zhao
Manli He
Dong Li
Congwei Gu
Matcha alleviates obesity by modulating gut microbiota and its metabolites
Current Research in Food Science
Matcha
Obesity
High-fat diet
Gut microbiota
Untargeted metabolomics
Correlation analysis
title Matcha alleviates obesity by modulating gut microbiota and its metabolites
title_full Matcha alleviates obesity by modulating gut microbiota and its metabolites
title_fullStr Matcha alleviates obesity by modulating gut microbiota and its metabolites
title_full_unstemmed Matcha alleviates obesity by modulating gut microbiota and its metabolites
title_short Matcha alleviates obesity by modulating gut microbiota and its metabolites
title_sort matcha alleviates obesity by modulating gut microbiota and its metabolites
topic Matcha
Obesity
High-fat diet
Gut microbiota
Untargeted metabolomics
Correlation analysis
url http://www.sciencedirect.com/science/article/pii/S2665927124001497
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